The Vesicle Protein SAM-4 Regulates the Processivity of Synaptic Vesicle Transport

Overview

Journal PLoS Genet

Specialty Genetics

Date 2014 Oct 21

PMID 25329901

Citations 30

Authors

Qun Zheng

Shikha Ahlawat

Anneliese Schaefer

Tim Mahoney

Sandhya P Koushika

Michael L Nonet

Affiliations

Soon will be listed here.

Abstract

Axonal transport of synaptic vesicles (SVs) is a KIF1A/UNC-104 mediated process critical for synapse development and maintenance yet little is known of how SV transport is regulated. Using C. elegans as an in vivo model, we identified SAM-4 as a novel conserved vesicular component regulating SV transport. Processivity, but not velocity, of SV transport was reduced in sam-4 mutants. sam-4 displayed strong genetic interactions with mutations in the cargo binding but not the motor domain of unc-104. Gain-of-function mutations in the unc-104 motor domain, identified in this study, suppress the sam-4 defects by increasing processivity of the SV transport. Genetic analyses suggest that SAM-4, SYD-2/liprin-α and the KIF1A/UNC-104 motor function in the same pathway to regulate SV transport. Our data support a model in which the SV protein SAM-4 regulates the processivity of SV transport.

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