Performance of the SD Bioline TB Ag MPT64 Rapid Test for Quick Confirmation of Mycobacterium Bovis Isolates from Animals

Overview

Journal J Vet Sci

Specialty Veterinary Medicine

Date 2014 Oct 2

PMID 25269718

Citations 3

Authors

Hyeon-Seop Byeon

Mi Jung Ji

Shin-Seok Kang

Sang Woo Kim

Seung-Cheol Kim

Song-Yong Park

Geehyuk Kim

Jiro Kim

Jang-Eun Cho

Bok Kyung Ku

Jae-Myung Kim

Bo-Young Jeon

Affiliations

Soon will be listed here.

Abstract

Mycobacterium (M.) bovis, a bacterium in the M. tuberculosis complex, is a causative agent of bovine tuberculosis, a contagious disease of animals. Mycobacterial culture is the gold standard for diagnosing bovine tuberculosis, but this technique is laborious and time-consuming. In the present study, performance of the SD Bioline TB Ag MPT4 Rapid test, an immunochromatographic assay, was evaluated using reference bacterial strains and M. bovis field isolates collected from animals. The SD MPT64 Rapid test produced positive results for 95.5% (63/66) of the M. bovis isolates from cattle and 97.9% (46/47) of the isolates from deer. Additionally, the test had a sensitivity of 96.5% (95% CI, 91.2-99.0), specificity of 100% (95% CI, 96.7-100.0), positive predictive value of 100% (95% CI, 96.7-100.0), and negative predictive value of 92.9% (95% CI, 82.7-98.0) for M. bovis isolates. In conclusion, the SD MPT64 Rapid test is simple to use and may be useful for quickly confirming the presence of M. bovis in animals.

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