Digital Image Analysis for DETCHIP Code Determination

Overview

Journal Signal Image Process

Date 2014 Oct 1

PMID 25267940

Citations 5

Authors

Marcus Lyon

Mark V Wilson

Kerry A Rouhier

David J Symonsbergen

Kiran Bastola

Ishwor Thapa

Andrea E Holmes

Sharmin M Sikich

Abby Jackson

Affiliations

Soon will be listed here.

Abstract

DETECHIP is a molecular sensing array used for identification of a large variety of substances. Previous methodology for the analysis of DETECHIP used human vision to distinguish color changes induced by the presence of the analyte of interest. This paper describes several analysis techniques using digital images of DETECHIP. Both a digital camera and flatbed desktop photo scanner were used to obtain Jpeg images. Color information within these digital images was obtained through the measurement of red-green-blue (RGB) values using software such as GIMP, Photoshop and ImageJ. Several different techniques were used to evaluate these color changes. It was determined that the flatbed scanner produced in the clearest and more reproducible images. Furthermore, codes obtained using a macro written for use within ImageJ showed improved consistency versus pervious methods.

Citing Articles

An Improved Comparison of Chemometric Analyses for the Identification of Acids and Bases With Colorimetric Sensor Arrays.

Kangas M, Wilson C, Burks R, Atwater J, Lukowicz R, Garver B Int J Chem. 2019; 10(2):36-55.

PMID: 31745401 PMC: 6863514. DOI: 10.5539/ijc.v10n2p36.

Comparative Chemometric Analysis for Classification of Acids and Bases via a Colorimetric Sensor Array.

Kangas M, Burks R, Atwater J, Lukowicz R, Garver B, Holmes A J Chemom. 2018; 32(2).

PMID: 29795964 PMC: 5962272. DOI: 10.1002/cem.2961.

Colorimetric Sensor Arrays for the Detection and Identification of Chemical Weapons and Explosives.

Kangas M, Burks R, Atwater J, Lukowicz R, Williams P, Holmes A Crit Rev Anal Chem. 2016; 47(2):138-153.

PMID: 27636675 PMC: 5351797. DOI: 10.1080/10408347.2016.1233805.

Reactive Arrays of Colorimetric Sensors for Metabolite and Steroid Identification.

Batres G, Jones T, Johnke H, Wilson M, Holmes A, Sikich S J Sens Technol. 2014; 4(1).

PMID: 25019034 PMC: 4091918. DOI: 10.4236/jst.2014.41001.

Detecting Concentration of Analytes with DETECHIP: A Molecular Sensing Array.

Johnke H, Batres G, Wilson M, Holmes A, Sikich S J Sens Technol. 2014; 3(3).

PMID: 24409399 PMC: 3883435. DOI: 10.4236/jst.2013.33015.

References

ONeal C, CROUCH D, Fatah A . Validation of twelve chemical spot tests for the detection of drugs of abuse. Forensic Sci Int. 2000; 109(3):189-201. DOI: 10.1016/s0379-0738(99)00235-2. View

Lin H, Suslick K . A colorimetric sensor array for detection of triacetone triperoxide vapor. J Am Chem Soc. 2010; 132(44):15519-21. DOI: 10.1021/ja107419t. View

Burks R, Pacquette S, Guericke M, Wilson M, Symonsbergen D, Lucas K . DETECHIP: a sensor for drugs of abuse. J Forensic Sci. 2010; 55(3):723-7. DOI: 10.1111/j.1556-4029.2010.01323.x. View

Kollroser M, Schober C . Simultaneous analysis of flunitrazepam and its major metabolites in human plasma by high performance liquid chromatography tandem mass spectrometry. J Pharm Biomed Anal. 2002; 28(6):1173-82. DOI: 10.1016/s0731-7085(02)00058-4. View

Morris J . Modified cobalt thiocyanate presumptive color test for ketamine hydrochloride. J Forensic Sci. 2007; 52(1):84-7. DOI: 10.1111/j.1556-4029.2006.00331.x. View

Steiner M, Meier R, Duerkop A, Wolfbeis O . Chromogenic sensing of biogenic amines using a chameleon probe and the red-green-blue readout of digital camera images. Anal Chem. 2011; 82(20):8402-5. DOI: 10.1021/ac102029j. View

Huang Q, He X, Ma C, Liu R, Yu S, Dayer C . Pharmacophore/receptor models for GABA(A)/BzR subtypes (alpha1beta3gamma2, alpha5beta3gamma2, and alpha6beta3gamma2) via a comprehensive ligand-mapping approach. J Med Chem. 2000; 43(1):71-95. DOI: 10.1021/jm990341r. View

Negrusz A, Moore C, Deitermann D, Lewis D, Kaleciak K, Kronstrand R . Highly sensitive micro-plate enzyme immunoassay screening and NCI-GC-MS confirmation of flunitrazepam and its major metabolite 7-aminoflunitrazepam in hair. J Anal Toxicol. 1999; 23(6):429-35. DOI: 10.1093/jat/23.6.429. View

Lim S, Musto C, Park E, Zhong W, Suslick K . A colorimetric sensor array for detection and identification of sugars. Org Lett. 2008; 10(20):4405-8. PMC: 2630291. DOI: 10.1021/ol801459k. View

10.

Feng L, Musto C, Kemling J, Lim S, Suslick K . A colorimetric sensor array for identification of toxic gases below permissible exposure limits. Chem Commun (Camb). 2010; 46(12):2037-9. PMC: 2976522. DOI: 10.1039/b926848k. View

11.

Rakow N, Suslick K . A colorimetric sensor array for odour visualization. Nature. 2000; 406(6797):710-3. DOI: 10.1038/35021028. View

12.

Feng L, Musto C, Suslick K . A simple and highly sensitive colorimetric detection method for gaseous formaldehyde. J Am Chem Soc. 2010; 132(12):4046-7. PMC: 2854577. DOI: 10.1021/ja910366p. View

13.

ElSohly M, Salamone S . Prevalence of drugs used in cases of alleged sexual assault. J Anal Toxicol. 1999; 23(3):141-6. DOI: 10.1093/jat/23.3.141. View

14.

Janzen M, Ponder J, Bailey D, Ingison C, Suslick K . Colorimetric sensor arrays for volatile organic compounds. Anal Chem. 2006; 78(11):3591-600. DOI: 10.1021/ac052111s. View