Viral Protein R of Human Immunodeficiency Virus Types 1 and 2 is Dispensable for Replication and Cytopathogenicity in Lymphoid Cells

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1989 Jul 1

PMID 2524599

Citations 46

Authors

D Dedera

W Hu

N Vander Heyden

L Ratner

Affiliations

Soon will be listed here.

Abstract

Viral protein R (VPR) is conserved in human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2). To assess its function, we have constructed mutations within the vpr coding regions of HIV-1 and HIV-2 predicted to express truncated VPR products. Infectious virus was produced by each proviral clone and showed similar replication kinetics and cytopathogenicity when compared with the corresponding parental proviral clone.

Citing Articles

HIV-1 Vpr combats the PU.1-driven antiviral response in primary human macrophages.

Virgilio M, Ramnani B, Chen T, Disbennett W, Lubow J, Welch J Nat Commun. 2024; 15(1):5514.

PMID: 38951492 PMC: 11217462. DOI: 10.1038/s41467-024-49635-w.

The Interplay Among HIV, LINE-1, and the Interferon Signaling System.

Zhao X, Zhao Y, Du J, Gao P, Zhao K Front Immunol. 2021; 12:732775.

PMID: 34566998 PMC: 8459832. DOI: 10.3389/fimmu.2021.732775.

HIV-1 Vpr antagonizes innate immune activation by targeting karyopherin-mediated NF-κB/IRF3 nuclear transport.

Khan H, Sumner R, Rasaiyaah J, Tan C, Rodriguez-Plata M, van Tulleken C Elife. 2020; 9.

PMID: 33300875 PMC: 7759385. DOI: 10.7554/eLife.60821.

Protein Arginine -methyltransferases 5 and 7 Promote HIV-1 Production.

Murakami H, Suzuki T, Tsuchiya K, Gatanaga H, Taura M, Kudo E Viruses. 2020; 12(3).

PMID: 32210193 PMC: 7150949. DOI: 10.3390/v12030355.

Vpr Enhances HIV-1 Env Processing and Virion Infectivity in Macrophages by Modulating TET2-Dependent IFITM3 Expression.

Wang Q, Su L mBio. 2019; 10(4).

PMID: 31431548 PMC: 6703422. DOI: 10.1128/mBio.01344-19.

References

Poiesz B, Ruscetti F, Gazdar A, Bunn P, Minna J, Gallo R . Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc Natl Acad Sci U S A. 1980; 77(12):7415-9. PMC: 350514. DOI: 10.1073/pnas.77.12.7415. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Kunkel T . Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci U S A. 1985; 82(2):488-92. PMC: 397064. DOI: 10.1073/pnas.82.2.488. View

Sonigo P, Alizon M, Staskus K, Klatzmann D, Cole S, Danos O . Nucleotide sequence of the visna lentivirus: relationship to the AIDS virus. Cell. 1985; 42(1):369-82. DOI: 10.1016/s0092-8674(85)80132-x. View

Oshimura M, Gilmer T, Barrett J . Nonrandom loss of chromosome 15 in Syrian hamster tumours induced by v-Ha-ras plus v-myc oncogenes. Nature. 1985; 316(6029):636-9. DOI: 10.1038/316636a0. View

Guyader M, Emerman M, Sonigo P, Clavel F, Montagnier L, Alizon M . Genome organization and transactivation of the human immunodeficiency virus type 2. Nature. 1987; 326(6114):662-9. DOI: 10.1038/326662a0. View

Chakrabarti L, Guyader M, Alizon M, Daniel M, Desrosiers R, Tiollais P . Sequence of simian immunodeficiency virus from macaque and its relationship to other human and simian retroviruses. Nature. 1987; 328(6130):543-7. DOI: 10.1038/328543a0. View

Fisher A, Ensoli B, Ivanoff L, Chamberlain M, Petteway S, Ratner L . The sor gene of HIV-1 is required for efficient virus transmission in vitro. Science. 1987; 237(4817):888-93. DOI: 10.1126/science.3497453. View

Wong-Staal F, Chanda P, Ghrayeb J . Human immunodeficiency virus: the eighth gene. AIDS Res Hum Retroviruses. 1987; 3(1):33-9. DOI: 10.1089/aid.1987.3.33. View

10.

Ratner L, Fisher A, Jagodzinski L, Mitsuya H, Liou R, Gallo R . Complete nucleotide sequences of functional clones of the AIDS virus. AIDS Res Hum Retroviruses. 1987; 3(1):57-69. DOI: 10.1089/aid.1987.3.57. View

11.

Fukasawa M, Miura T, Hasegawa A, Morikawa S, Tsujimoto H, Miki K . Sequence of simian immunodeficiency virus from African green monkey, a new member of the HIV/SIV group. Nature. 1988; 333(6172):457-61. DOI: 10.1038/333457a0. View

12.

Henderson L, Sowder R, Copeland T, Benveniste R, Oroszlan S . Isolation and characterization of a novel protein (X-ORF product) from SIV and HIV-2. Science. 1988; 241(4862):199-201. DOI: 10.1126/science.3388031. View

13.

Kappes J, Morrow C, Lee S, Jameson B, Kent S, HOOD L . Identification of a novel retroviral gene unique to human immunodeficiency virus type 2 and simian immunodeficiency virus SIVMAC. J Virol. 1988; 62(9):3501-5. PMC: 253477. DOI: 10.1128/JVI.62.9.3501-3505.1988. View

14.

Yu X, Ito S, Essex M, Lee T . A naturally immunogenic virion-associated protein specific for HIV-2 and SIV. Nature. 1988; 335(6187):262-5. DOI: 10.1038/335262a0. View

15.

Haseltine W . Replication and pathogenesis of the AIDS virus. J Acquir Immune Defic Syndr (1988). 1988; 1(3):217-40. View

16.

Ratner L, Haseltine W, Patarca R, Livak K, Starcich B, Josephs S . Complete nucleotide sequence of the AIDS virus, HTLV-III. Nature. 1985; 313(6000):277-84. DOI: 10.1038/313277a0. View