Human Fc Gamma RIII: Cloning, Expression, and Identification of the Chromosomal Locus of Two Fc Receptors for IgG

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1989 Feb 1

PMID 2521732

Citations 27

Authors

G A Peltz

H O Grundy

R V Lebo

H Yssel

G S Barsh

K W Moore

Affiliations

Soon will be listed here.

Abstract

A cDNA clone encoding a human receptor for the Fc portion of IgG (Fc gamma R), Fc gamma RIII or CD16, was isolated from a human leukocyte library by a transient expression-immunoselection procedure. This cDNA (pGP5) encodes a 46-kDa phosphatidylinositol-linked cell surface protein with CD16 determinants and affinity for human IgG. The deduced protein sequence is most homologous to the murine receptor Fc gamma RII alpha, with slightly less homology to the human receptors Fc gamma RII and Fc epsilon RI. The cDNA hybridizes to a 2.2 kilobase mRNA in human leukocytes and a cloned human natural killer cell line. Fc gamma RIII is mapped to chromosome 1 by spot-blot analysis of sorted human chromosomes. Hybridization of Fc gamma RII and Fc gamma RIII probes to restriction digests of human genomic DNA separated by pulsed-field gel electrophoresis demonstrates physical linkage of the two genes within a maximum distance of 200 kilobases. The results identify a locus for at least two Fc gamma R genes on human chromosome 1.

Citing Articles

A Comprehensive Review of Fc Gamma Receptors and Their Role in Systemic Lupus Erythematosus.

Sepulveda-Delgado J, Llorente L, Hernandez-Dono S Int J Mol Sci. 2025; 26(5).

PMID: 40076476 PMC: 11899777. DOI: 10.3390/ijms26051851.

Interaction with cellular CD4 exposes HIV-1 envelope epitopes targeted by antibody-dependent cell-mediated cytotoxicity.

Veillette M, Desormeaux A, Medjahed H, Gharsallah N, Coutu M, Baalwa J J Virol. 2013; 88(5):2633-44.

PMID: 24352444 PMC: 3958102. DOI: 10.1128/JVI.03230-13.

Dimers and multimers of monoclonal IgG1 exhibit higher in vitro binding affinities to Fcgamma receptors.

Luo Y, Lu Z, Raso S, Entrican C, Tangarone B MAbs. 2010; 1(5):491-504.

PMID: 20065648 PMC: 2759499. DOI: 10.4161/mabs.1.5.9631.

Glycosylation of FcgammaRIII in N163 as mechanism of regulating receptor affinity.

Drescher B, Witte T, Schmidt R Immunology. 2003; 110(3):335-40.

PMID: 14632661 PMC: 1783064. DOI: 10.1046/j.1365-2567.2003.01743.x.

Distribution of variant genotypes of Fc gamma receptor IIIa in healthy Chinese population of Zhengzhou City.

Wang J, Feng J, Zhang L, Hu Y, Luan B, Yue W J Huazhong Univ Sci Technolog Med Sci. 2003; 23(3):239-41.

PMID: 14526422 DOI: 10.1007/BF02829502.

References

Kulczycki Jr A . Human neutrophils and eosinophils have structurally distinct Fc gamma receptors. J Immunol. 1984; 133(2):849-54. View

Razzaque A, Mizusawa H, Seidman M . Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells. Proc Natl Acad Sci U S A. 1983; 80(10):3010-4. PMC: 393963. DOI: 10.1073/pnas.80.10.3010. View

Salmon J, Kimberly R, Gibofsky A, Fotino M . Altered phagocytosis by monocytes from HLA-DR2 and DR3-positive healthy adults is Fc gamma receptor specific. J Immunol. 1986; 136(10):3625-30. View

Clarkson S, Bussel J, Kimberly R, Valinsky J, Nachman R, Unkeless J . Treatment of refractory immune thrombocytopenic purpura with an anti-Fc gamma-receptor antibody. N Engl J Med. 1986; 314(19):1236-9. DOI: 10.1056/NEJM198605083141907. View

Malavasi F, Tetta C, Funaro A, Bellone G, Ferrero E, Franzone A . Fc receptor triggering induces expression of surface activation antigens and release of platelet-activating factor in large granular lymphocytes. Proc Natl Acad Sci U S A. 1986; 83(8):2443-7. PMC: 323314. DOI: 10.1073/pnas.83.8.2443. View

von Heijne G . A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 1986; 14(11):4683-90. PMC: 311474. DOI: 10.1093/nar/14.11.4683. View

Gresham H, Clement L, Lehmeyer J, Griffin Jr F, Volanakis J . Stimulation of human neutrophil Fc receptor-mediated phagocytosis by a low molecular weight cytokine. J Immunol. 1986; 137(3):868-75. View

Clarkson S, Kimberly R, Valinsky J, Witmer M, Bussel J, Nachman R . Blockade of clearance of immune complexes by an anti-Fc gamma receptor monoclonal antibody. J Exp Med. 1986; 164(2):474-89. PMC: 2188226. DOI: 10.1084/jem.164.2.474. View

Ravetch J, Luster A, Weinshank R, Kochan J, PAVLOVEC A, Portnoy D . Structural heterogeneity and functional domains of murine immunoglobulin G Fc receptors. Science. 1986; 234(4777):718-25. DOI: 10.1126/science.2946078. View

10.

van de Griend R, Bolhuis R, Stoter G, Roozemond R . Regulation of cytolytic activity in CD3- and CD3+ killer cell clones by monoclonal antibodies (anti-CD16, anti-CD2, anti-CD3) depends on subclass specificity of target cell IgG-FcR. J Immunol. 1987; 138(10):3137-44. View

11.

Seed B, Aruffo A . Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure. Proc Natl Acad Sci U S A. 1987; 84(10):3365-9. PMC: 304871. DOI: 10.1073/pnas.84.10.3365. View

12.

Lebo R, Anderson L, Lau Y, Flandermeyer R, Kan Y . Flow-sorting analysis of normal and abnormal human genomes. Cold Spring Harb Symp Quant Biol. 1986; 51 Pt 1:169-76. DOI: 10.1101/sqb.1986.051.01.020. View

13.

Khayat D, Geffrier C, Yoon S, Scigliano E, Soubrane C, Weil M . Soluble circulating Fc gamma receptors in human serum. A new ELISA assay for specific and quantitative detection. J Immunol Methods. 1987; 100(1-2):235-41. DOI: 10.1016/0022-1759(87)90194-3. View

14.

Tabor S, Richardson C . DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc Natl Acad Sci U S A. 1987; 84(14):4767-71. PMC: 305186. DOI: 10.1073/pnas.84.14.4767. View

15.

Lawley T, Hall R, Fauci A, Katz S, Hamburger M, Frank M . Defective Fc-receptor functions associated with the HLA-B8/DRw3 haplotype: studies in patients with dermatitis herpetiformis and normal subjects. N Engl J Med. 1981; 304(4):185-92. DOI: 10.1056/NEJM198101223040401. View

16.

Gluzman Y . SV40-transformed simian cells support the replication of early SV40 mutants. Cell. 1981; 23(1):175-82. DOI: 10.1016/0092-8674(81)90282-8. View

17.

Fridman W, Rabourdin-Combe C, Neauport-Sautes C, Gisler R . Characterization and function of T cell Fc gamma receptor. Immunol Rev. 1981; 56:51-88. DOI: 10.1111/j.1600-065x.1981.tb01047.x. View

18.

Bell G, KARAM J, Rutter W . Polymorphic DNA region adjacent to the 5' end of the human insulin gene. Proc Natl Acad Sci U S A. 1981; 78(9):5759-63. PMC: 348853. DOI: 10.1073/pnas.78.9.5759. View

19.

Revillard J . Selective suppression of human B lymphocyte differentiation into IgG-producing cells by soluble Fc gamma receptors. J Immunol. 1982; 129(1):150-2. View

20.

Fleit H, Wright S, Unkeless J . Human neutrophil Fc gamma receptor distribution and structure. Proc Natl Acad Sci U S A. 1982; 79(10):3275-9. PMC: 346398. DOI: 10.1073/pnas.79.10.3275. View