Stem Cell Properties and Neural Differentiation of Sheep Amniotic Epithelial Cells

Overview

Journal Neural Regen Res

Date 2014 Sep 11

PMID 25206415

Citations 2

Authors

Xuemin Zhu

Xiumei Wang

Guifang Cao

Fengjun Liu

Yinfeng Yang

Xiaonan Li

Yuling Zhang

Yan Mi

Junping Liu

Lingli Zhang

Affiliations

Soon will be listed here.

Abstract

This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that the sheep amniotic epithelial cells were positive for the embryonic stem cell marker proteins SSEA-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, and the totipotency-associated genes Oct-4, Sox-2 and Rex-1, but negative for Nanog. Amniotic epithelial cells expressed β-III-tubulin, glial fibrillary acidic protein, nestin and microtubule-associated protein-2 at 28 days after induction with serum-free neurobasal-A medium containing B-27. Thus, sheep amniotic epithelial cells could differentiate into neurons expressing β-III-tubulin and microtubule-associated protein-2, and glial-like cells expressing glial fibrillary acidic protein, under specific conditions.

Citing Articles

Placental Stem Cells from Domestic Animals: Translational Potential and Clinical Relevance.

Barboni B, Russo V, Berardinelli P, Mauro A, Valbonetti L, Sanyal H Cell Transplant. 2018; 27(1):93-116.

PMID: 29562773 PMC: 6434480. DOI: 10.1177/0963689717724797.

Phenotype and multipotency of rabbit (Oryctolagus cuniculus) amniotic stem cells.

Borghesi J, Mario L, Carreira A, Miglino M, Favaron P Stem Cell Res Ther. 2017; 8(1):27.

PMID: 28173846 PMC: 5297200. DOI: 10.1186/s13287-016-0468-z.

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