Phenotypic Detection of Carbapenemase-producing Enterobacteriaceae by Use of Matrix-assisted Laser Desorption Ionization-time of Flight Mass Spectrometry and the Carba NP Test

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2014 Sep 5

PMID 25187633

Citations 24

Authors

James Knox

Snehal Jadhav

Danielle Sevior

Alex Agyekum

Margaret Whipp

Lynette Waring

Jonathan Iredell

Enzo Palombo

Affiliations

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Abstract

We compared the diagnostic accuracy of the Carba NP test with that of a straightforward matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) method for detecting carbapenemase-producing Enterobacteriaceae (CPE). Using PCR as the reference method, both tests demonstrated a sensitivity of 87% and a specificity of 100%. MALDI-TOF MS offers a potential alternative for the rapid detection of CPE in the clinical laboratory setting.

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References

Woodford N, Ellington M, Coelho J, Turton J, Ward M, Brown S . Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. Int J Antimicrob Agents. 2006; 27(4):351-3. DOI: 10.1016/j.ijantimicag.2006.01.004. View

Naas T, Cotellon G, Ergani A, Nordmann P . Real-time PCR for detection of blaOXA-48 genes from stools. J Antimicrob Chemother. 2012; 68(1):101-4. DOI: 10.1093/jac/dks340. View

Mushtaq S, Irfan S, Sarma J, Doumith M, Pike R, Pitout J . Phylogenetic diversity of Escherichia coli strains producing NDM-type carbapenemases. J Antimicrob Chemother. 2011; 66(9):2002-5. DOI: 10.1093/jac/dkr226. View

Bisiklis A, Papageorgiou F, Frantzidou F, Alexiou-Daniel S . Specific detection of blaVIM and blaIMP metallo-beta-lactamase genes in a single real-time PCR. Clin Microbiol Infect. 2007; 13(12):1201-3. DOI: 10.1111/j.1469-0691.2007.01832.x. View

Yan J, Hsueh P, Ko W, Luh K, Tsai S, Wu H . Metallo-beta-lactamases in clinical Pseudomonas isolates in Taiwan and identification of VIM-3, a novel variant of the VIM-2 enzyme. Antimicrob Agents Chemother. 2001; 45(8):2224-8. PMC: 90635. DOI: 10.1128/AAC.45.8.2224-2228.2001. View

Dortet L, Brechard L, Poirel L, Nordmann P . Impact of the isolation medium for detection of carbapenemase-producing Enterobacteriaceae using an updated version of the Carba NP test. J Med Microbiol. 2014; 63(Pt 5):772-776. DOI: 10.1099/jmm.0.071340-0. View

Poirel L, Walsh T, Cuvillier V, Nordmann P . Multiplex PCR for detection of acquired carbapenemase genes. Diagn Microbiol Infect Dis. 2011; 70(1):119-23. DOI: 10.1016/j.diagmicrobio.2010.12.002. View

Kempf M, Bakour S, Flaudrops C, Berrazeg M, Brunel J, Drissi M . Rapid detection of carbapenem resistance in Acinetobacter baumannii using matrix-assisted laser desorption ionization-time of flight mass spectrometry. PLoS One. 2012; 7(2):e31676. PMC: 3280980. DOI: 10.1371/journal.pone.0031676. View

Endimiani A, Carias L, Hujer A, Bethel C, Hujer K, Perez F . Presence of plasmid-mediated quinolone resistance in Klebsiella pneumoniae isolates possessing blaKPC in the United States. Antimicrob Agents Chemother. 2008; 52(7):2680-2. PMC: 2443894. DOI: 10.1128/AAC.00158-08. View

10.

Yong D, Toleman M, Giske C, Cho H, Sundman K, Lee K . Characterization of a new metallo-beta-lactamase gene, bla(NDM-1), and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob Agents Chemother. 2009; 53(12):5046-54. PMC: 2786356. DOI: 10.1128/AAC.00774-09. View

11.

Tijet N, Boyd D, Patel S, Mulvey M, Melano R . Evaluation of the Carba NP test for rapid detection of carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2013; 57(9):4578-80. PMC: 3754310. DOI: 10.1128/AAC.00878-13. View

12.

Hornsey M, Phee L, Wareham D . A novel variant, NDM-5, of the New Delhi metallo-β-lactamase in a multidrug-resistant Escherichia coli ST648 isolate recovered from a patient in the United Kingdom. Antimicrob Agents Chemother. 2011; 55(12):5952-4. PMC: 3232805. DOI: 10.1128/AAC.05108-11. View

13.

Ginn A, Zong Z, Wiklendt A, Thomas L, Merlino J, Gottlieb T . Limited diversity in the gene pool allows prediction of third-generation cephalosporin and aminoglycoside resistance in Escherichia coli and Klebsiella pneumoniae. Int J Antimicrob Agents. 2013; 42(1):19-26. DOI: 10.1016/j.ijantimicag.2013.03.003. View

14.

Huang T, Berhin C, Bogaerts P, Glupczynski Y . Comparative evaluation of two chromogenic tests for rapid detection of carbapenemase in Enterobacteriaceae and in Pseudomonas aeruginosa isolates. J Clin Microbiol. 2014; 52(8):3060-3. PMC: 4136138. DOI: 10.1128/JCM.00643-14. View