Rapid Detection of MDR-Mycobacterium Tuberculosis Using Modified PCR-SSCP from Clinical Specimens

Overview

Journal Asian Pac J Trop Biomed

Specialty Tropical Medicine

Date 2014 Sep 4

PMID 25183075

Citations 6

Authors

Imani Fooladi Abbas Ali

Farzam Babak

Mousavi Seyed Fazlollah

Jonaidi Jafari Nematollah

Affiliations

Soon will be listed here.

Abstract

Objective: To design a rapid test to detect the rifampin (RIF) and isoniazid (INH) resistant mutant based on polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique that analyzes the katG, rpoB genes.

Methods: Biochemical test as well as IS6110 targeting PCR revealed 103 clinical samples were tuberculosis. To determine the susceptibility of isolates to anti TB drugs, the proportional method was used. Mutations presented within the amplified products of the katG, rpoB genes were evaluated by SSCP.

Results: Using proportional method, 12 (11.6%) and 9 (8.7%) isolates were resistant respectively to INH and RIF and 9 (8.7%) isolates showed resistance to both drug (multi-drug resistant tuberculosis). Three (2.9%) multi-drug resistant tuberculosis and two INH resistant isolates were detected by the PCR-SSCP and sequencing. The sensitivity and specificity of PCR-SSCP for multi-drug resistant isolates were 33% and 100%, respectively.

Conclusions: Complete agreement between SSCP and sequencing can indicate that resistance-associated mutations have occurred in other genes except our considered genes.

Citing Articles

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PMID: 38560029 PMC: 10981085. DOI: 10.1016/j.jctube.2024.100430.

Rifampicin-resistant tuberculosis in Iran: A systematic review and meta-analysis.

Bahraminia F, Azimi T, Zangiabadian M, Nasiri M, Goudarzi M, Dadashi M Iran J Basic Med Sci. 2021; 24(6):720-725.

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Systematic Review of Mutations Associated with Isoniazid Resistance Points to Continuing Evolution and Subsequent Evasion of Molecular Detection, and Potential for Emergence of Multidrug Resistance in Clinical Strains of Mycobacterium tuberculosis.

Valafar S Antimicrob Agents Chemother. 2020; 65(3).

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Use of DNA microarray chips for the rapid detection of resistance to rifampicin and isoniazid.

Tang P, Wang X, Shen X, Shi M, Zhu X, Yu X Exp Ther Med. 2017; 13(5):2332-2338.

PMID: 28565846 PMC: 5443298. DOI: 10.3892/etm.2017.4250.

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