Comparative Evaluation of Antimicrobial Efficacy of Triple Antibiotic Paste and Calcium Hydroxide Using Chitosan As Carrier Against Candida Albicans and Enterococcus Faecalis: An in Vitro Study

Overview

Journal J Conserv Dent

Specialty Dentistry

Date 2014 Aug 16

PMID 25125845

Citations 22

Authors

Jaheer Shaik

Roopadevi Garlapati

Bolla Nagesh

Varri Sujana

Thumu Jayaprakash

Sudhakar Naidu

Affiliations

Soon will be listed here.

Abstract

Aim: To analyze the sustained release of intracanal medicaments with or without a carrier and testing their antimicrobial efficacy in root canal against Candida albicans and Enterococcus faecalis.

Materials And Methods: A total of 80 single-rooted anterior teeth were selected, root canal preparation was done, and teeth were divided into two halves and contaminated with C. albicans and E. faecalis, which were further divided into four test groups each according to intracanal medicaments used. Chitosan was used as vehicle for triple antibiotic paste (TAP) and calcium hydroxide and antimicrobial assessment was performed on second and seventh day. Dentine samples were collected after each.

Results: All four medicaments used in this study showed antifungal and antibacterial activity. Group II (TAP + chitosan) and Group III (Ca(OH)2 + chitosan) had the higher antifungal and antibacterial activity compared with two other groups.

Conclusion: Combination of TAP + chitosan and Ca(OH)2 + chitosan produced better results compared with the combination of medicaments with saline.

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References

Raafat D, Sahl H . Chitosan and its antimicrobial potential--a critical literature survey. Microb Biotechnol. 2011; 2(2):186-201. PMC: 3815839. DOI: 10.1111/j.1751-7915.2008.00080.x. View

Ozan U, Er K . Endodontic treatment of a large cyst-like periradicular lesion using a combination of antibiotic drugs: a case report. J Endod. 2005; 31(12):898-900. DOI: 10.1097/01.don.0000164129.74235.8e. View

Jadhav G, Shah N, Logani A . Comparative outcome of revascularization in bilateral, non-vital, immature maxillary anterior teeth supplemented with or without platelet rich plasma: A case series. J Conserv Dent. 2013; 16(6):568-72. PMC: 3842730. DOI: 10.4103/0972-0707.120932. View

Ballal N, Kundabala M, Bhat K, Acharya S, Ballal M, Kumar R . Susceptibility of Candida albicans and Enterococcus faecalis to Chitosan, Chlorhexidine gluconate and their combination in vitro. Aust Endod J. 2009; 35(1):29-33. DOI: 10.1111/j.1747-4477.2008.00126.x. View

Saber S, El-Hady S . Development of an intracanal mature Enterococcus faecalis biofilm and its susceptibility to some antimicrobial intracanal medications; an in vitro study. Eur J Dent. 2012; 6(1):43-50. PMC: 3252799. View

Wang J, Zeng Z, Xiao R, Xie T, Zhou G, Zhan X . Recent advances of chitosan nanoparticles as drug carriers. Int J Nanomedicine. 2011; 6:765-74. PMC: 3090273. DOI: 10.2147/IJN.S17296. View

Krithikadatta J, Indira R, Dorothykalyani A . Disinfection of dentinal tubules with 2% chlorhexidine, 2% metronidazole, bioactive glass when compared with calcium hydroxide as intracanal medicaments. J Endod. 2007; 33(12):1473-6. DOI: 10.1016/j.joen.2007.08.016. View

Hoshino E, Sato I, Uematsu H, Sato M, Kota K, Iwaku M . In-vitro antibacterial susceptibility of bacteria taken from infected root dentine to a mixture of ciprofloxacin, metronidazole and minocycline. Int Endod J. 1996; 29(2):125-30. DOI: 10.1111/j.1365-2591.1996.tb01173.x. View

Bystrom A, Sundqvist G . The antibacterial action of sodium hypochlorite and EDTA in 60 cases of endodontic therapy. Int Endod J. 1985; 18(1):35-40. DOI: 10.1111/j.1365-2591.1985.tb00416.x. View

10.

Ballal N, Shavi G, Kumar R, Kundabala M, Bhat K . In vitro sustained release of calcium ions and pH maintenance from different vehicles containing calcium hydroxide. J Endod. 2010; 36(5):862-6. DOI: 10.1016/j.joen.2009.12.021. View

11.

Sato I, Kota K, Iwaku M, Hoshino E . Sterilization of infected root-canal dentine by topical application of a mixture of ciprofloxacin, metronidazole and minocycline in situ. Int Endod J. 1996; 29(2):118-24. DOI: 10.1111/j.1365-2591.1996.tb01172.x. View

12.

Elsaka S, Elnaghy A . Antibacterial activity of calcium hydroxide combined with chitosan solutions and the outcomes on the bond strength of RealSeal sealer to radicular dentin. J Biomed Res. 2013; 26(3):193-9. PMC: 3596069. DOI: 10.7555/JBR.26.20110136. View

13.

Turk B, Sen B, Ozturk T . In vitro antimicrobial activity of calcium hydroxide mixed with different vehicles against Enterococcus faecalis and Candida albicans. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 108(2):297-301. DOI: 10.1016/j.tripleo.2009.03.029. View

14.

Adl A, Shojaee N, Motamedifar M . A Comparison between the Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Against Entrococcus Faecalis. Iran Endod J. 2012; 7(3):149-55. PMC: 3467137. View

15.

Aggarwal V, Miglani S, Singla M . Conventional apexification and revascularization induced maturogenesis of two non-vital, immature teeth in same patient: 24 months follow up of a case. J Conserv Dent. 2012; 15(1):68-72. PMC: 3284018. DOI: 10.4103/0972-0707.92610. View

16.

Sundqvist G, Figdor D, Persson S, Sjogren U . Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998; 85(1):86-93. DOI: 10.1016/s1079-2104(98)90404-8. View

17.

Taneja S, Kumari M, Parkash H . Nonsurgical healing of large periradicular lesions using a triple antibiotic paste: A case series. Contemp Clin Dent. 2011; 1(1):31-5. PMC: 3220065. DOI: 10.4103/0976-237X.62519. View

18.

Hagihara Y, Kaminishi H, Cho T, Tanaka M, Kaita H . Degradation of human dentine collagen by an enzyme produced by the yeast Candida albicans. Arch Oral Biol. 1988; 33(8):617-9. DOI: 10.1016/0003-9969(88)90138-0. View

19.

Pramila R, Muthu M . Regeneration potential of pulp-dentin complex: Systematic review. J Conserv Dent. 2012; 15(2):97-103. PMC: 3339021. DOI: 10.4103/0972-0707.94571. View

20.

Siqueira Jr J, de Uzeda M . Influence of different vehicles on the antibacterial effects of calcium hydroxide. J Endod. 1999; 24(10):663-5. DOI: 10.1016/S0099-2399(98)80151-9. View