Detection of Kaposi's Sarcoma Associated Herpesvirus Nucleic Acids Using a Smartphone Accessory

Overview

Journal Lab Chip

Publisher Royal Society of Chemistry

Specialties Biotechnology
Chemistry

Date 2014 Aug 14

PMID 25117534

Citations 10

Authors

Matthew Mancuso

Ethel Cesarman

David Erickson

Affiliations

Soon will be listed here.

Abstract

Kaposi's sarcoma (KS) is an infectious cancer occurring in immune-compromised patients, caused by Kaposi's sarcoma associated herpesvirus (KSHV). Our vision is to simplify the process of KS diagnosis through the creation of a smartphone based point-of-care system capable of yielding an actionable diagnostic readout starting from a raw biopsy sample. In this work we develop the sensing mechanism for the overall system, a smartphone accessory capable of detecting KSHV nucleic acids. The accessory reads out microfluidic chips filled with a colorimetric nanoparticle assay targeted at KSHV. We calculate that our final device can read out gold nanoparticle solutions with an accuracy of 0.05 OD, and we demonstrate that it can detect DNA sequences from KSHV down to 1 nM. We believe that through integration with our previously developed components, a smartphone based system like the one studied here can provide accurate detection information, as well as a simple platform for field based clinical diagnosis and research.

Citing Articles

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References

Isikman S, Bishara W, Sikora U, Yaglidere O, Yeah J, Ozcan A . Field-portable lensfree tomographic microscope. Lab Chip. 2011; 11(13):2222-30. PMC: 3154753. DOI: 10.1039/c1lc20127a. View

Greenbaum A, Sikora U, Ozcan A . Field-portable wide-field microscopy of dense samples using multi-height pixel super-resolution based lensfree imaging. Lab Chip. 2012; 12(7):1242-5. DOI: 10.1039/c2lc21072j. View

Gallegos D, Long K, Yu H, Clark P, Lin Y, George S . Label-free biodetection using a smartphone. Lab Chip. 2013; 13(11):2124-32. DOI: 10.1039/c3lc40991k. View

Shen L, Hagen J, Papautsky I . Point-of-care colorimetric detection with a smartphone. Lab Chip. 2012; 12(21):4240-3. DOI: 10.1039/c2lc40741h. View

Storhoff J, Lucas A, Garimella V, Bao Y, Muller U . Homogeneous detection of unamplified genomic DNA sequences based on colorimetric scatter of gold nanoparticle probes. Nat Biotechnol. 2004; 22(7):883-7. PMC: 1201475. DOI: 10.1038/nbt977. View

Lee M, Yaglidere O, Ozcan A . Field-portable reflection and transmission microscopy based on lensless holography. Biomed Opt Express. 2011; 2(9):2721-30. PMC: 3184880. DOI: 10.1364/BOE.2.002721. View

McDonald J, Whitesides G . Poly(dimethylsiloxane) as a material for fabricating microfluidic devices. Acc Chem Res. 2002; 35(7):491-9. DOI: 10.1021/ar010110q. View

Pitchenik A, Fischl M, Dickinson G, Becker D, FOURNIER A, OConnell M . Opportunistic infections and Kaposi's sarcoma among Haitians: evidence of a new acquired immunodeficiency state. Ann Intern Med. 1983; 98(3):277-84. DOI: 10.7326/0003-4819-98-3-277. View

Hymes K, Cheung T, Greene J, Prose N, Marcus A, Ballard H . Kaposi's sarcoma in homosexual men-a report of eight cases. Lancet. 1981; 2(8247):598-600. DOI: 10.1016/s0140-6736(81)92740-9. View

10.

Ablashi D, Chatlynne L, Whitman Jr J, Cesarman E . Spectrum of Kaposi's sarcoma-associated herpesvirus, or human herpesvirus 8, diseases. Clin Microbiol Rev. 2002; 15(3):439-64. PMC: 118087. DOI: 10.1128/CMR.15.3.439-464.2002. View

11.

Mesri E, Cesarman E, Boshoff C . Kaposi's sarcoma and its associated herpesvirus. Nat Rev Cancer. 2010; 10(10):707-19. PMC: 4721662. DOI: 10.1038/nrc2888. View

12.

Chang Y, Cesarman E, Pessin M, Lee F, Culpepper J, Knowles D . Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma. Science. 1994; 266(5192):1865-9. DOI: 10.1126/science.7997879. View

13.

Friedman-Kien A . Disseminated Kaposi's sarcoma syndrome in young homosexual men. J Am Acad Dermatol. 1981; 5(4):468-71. DOI: 10.1016/s0190-9622(81)80010-2. View

14.

Rosi N, Mirkin C . Nanostructures in biodiagnostics. Chem Rev. 2005; 105(4):1547-62. DOI: 10.1021/cr030067f. View

15.

Tappero J, Conant M, Wolfe S, Berger T . Kaposi's sarcoma. Epidemiology, pathogenesis, histology, clinical spectrum, staging criteria and therapy. J Am Acad Dermatol. 1993; 28(3):371-95. DOI: 10.1016/0190-9622(93)70057-z. View

16.

Lillehoj P, Wei F, Ho C . A self-pumping lab-on-a-chip for rapid detection of botulinum toxin. Lab Chip. 2010; 10(17):2265-70. DOI: 10.1039/c004885b. View

17.

Wei Q, Qi H, Luo W, Tseng D, Ki S, Wan Z . Fluorescent imaging of single nanoparticles and viruses on a smart phone. ACS Nano. 2013; 7(10):9147-55. PMC: 3951925. DOI: 10.1021/nn4037706. View

18.

Mao X, Huang T . Microfluidic diagnostics for the developing world. Lab Chip. 2012; 12(8):1412-6. PMC: 6365145. DOI: 10.1039/c2lc90022j. View

19.

Mancuso M, Jiang L, Cesarman E, Erickson D . Multiplexed colorimetric detection of Kaposi's sarcoma associated herpesvirus and Bartonella DNA using gold and silver nanoparticles. Nanoscale. 2013; 5(4):1678-86. PMC: 3581344. DOI: 10.1039/c3nr33492a. View

20.

Tappero J, Koehler J . Images in clinical medicine. Bacillary angiomatosis or Kaposi's sarcoma?. N Engl J Med. 1997; 337(26):1888. DOI: 10.1056/NEJM199712253372605. View