Electrochemical Quantification of the Antioxidant Capacity of Medicinal Plants Using Biosensors

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2014 Aug 12

PMID 25111237

Citations 10

Authors

Erika Rodriguez-Sevilla

Maria-Teresa Ramirez-Silva

Mario Romero-Romo

Pedro Ibarra-Escutia

Manuel Palomar-Pardave

Affiliations

Soon will be listed here.

Abstract

The working area of a screen-printed electrode, SPE, was modified with the enzyme tyrosinase (Tyr) using different immobilization methods, namely entrapment with water-soluble polyvinyl alcohol (PVA), cross-linking using glutaraldehyde (GA), and cross-linking using GA and human serum albumin (HSA); the resulting electrodes were termed SPE/Tyr/PVA, SPE/Tyr/GA and SPE/Tyr/HSA/GA, respectively. These biosensors were characterized by means of amperometry and EIS techniques. From amperometric evaluations, the apparent Michaelis-Menten constant, Km', of each biosensor was evaluated while the respective charge transfer resistance, Rct, was assessed from impedance measurements. It was found that the SPE/Tyr/GA had the smallest Km' (57 ± 7) µM and Rct values. This electrode also displayed both the lowest detection and quantification limits for catechol quantification. Using the SPE/Tyr/GA, the Trolox Equivalent Antioxidant Capacity (TEAC) was determined from infusions prepared with "mirto" (Salvia microphylla), "hHierba dulce" (Lippia dulcis) and "salve real" (Lippia alba), medicinal plants commonly used in Mexico.

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References

Fenoll L, Rodriguez-Lopez J, Garcia-Molina F, Garcia-Canovas F, Tudela J . Michaelis constants of mushroom tyrosinase with respect to oxygen in the presence of monophenols and diphenols. Int J Biochem Cell Biol. 2002; 34(4):332-6. DOI: 10.1016/s1357-2725(01)00133-9. View

Mayorga-Martinez C, Cadevall M, Guix M, Ros J, Merkoci A . Bismuth nanoparticles for phenolic compounds biosensing application. Biosens Bioelectron. 2012; 40(1):57-62. DOI: 10.1016/j.bios.2012.06.010. View

Li X, Shen L, Zhang D, Qi H, Gao Q, Ma F . Electrochemical impedance spectroscopy for study of aptamer-thrombin interfacial interactions. Biosens Bioelectron. 2008; 23(11):1624-30. DOI: 10.1016/j.bios.2008.01.029. View

Stanca S, Popescu I . Phenols monitoring and Hill coefficient evaluation using tyrosinase-based amperometric biosensors. Bioelectrochemistry. 2004; 64(1):47-52. DOI: 10.1016/j.bioelechem.2004.02.004. View

Riley P . Oxidation of monohydric phenol substrates by tyrosinase. An oximetric study. Biochem J. 1992; 288 ( Pt 1):63-7. PMC: 1132080. DOI: 10.1042/bj2880063. View

Kintzios S, Papageorgiou K, Yiakoumettis I, Baricevic D, Kusar A . Evaluation of the antioxidants activities of four Slovene medicinal plant species by traditional and novel biosensory assays. J Pharm Biomed Anal. 2010; 53(3):773-6. DOI: 10.1016/j.jpba.2010.05.013. View

ElKaoutit M, Naranjo-Rodriguez I, Temsamani K, Hernandez-Artiga M, Bellido-Milla D, Cisneros J . A comparison of three amperometric phenoloxidase-Sonogel-Carbon based biosensors for determination of polyphenols in beers. Food Chem. 2015; 110(4):1019-24. DOI: 10.1016/j.foodchem.2008.03.006. View

Shleev S, Tkac J, Christenson A, Ruzgas T, Yaropolov A, Whittaker J . Direct electron transfer between copper-containing proteins and electrodes. Biosens Bioelectron. 2005; 20(12):2517-54. DOI: 10.1016/j.bios.2004.10.003. View

Espin J, Garcia-Ruiz P, Tudela J, Garcia-Canovas F . Study of stereospecificity in mushroom tyrosinase. Biochem J. 1998; 331 ( Pt 2):547-51. PMC: 1219387. DOI: 10.1042/bj3310547. View

10.

Perez S, Bartroli J, Fabregas E . Amperometric biosensor for the determination of histamine in fish samples. Food Chem. 2013; 141(4):4066-72. DOI: 10.1016/j.foodchem.2013.06.125. View

11.

Michaelis L, Menten M, Johnson K, Goody R . The original Michaelis constant: translation of the 1913 Michaelis-Menten paper. Biochemistry. 2011; 50(39):8264-9. PMC: 3381512. DOI: 10.1021/bi201284u. View

12.

Peterson N . Analysis of dual transport systems by means of Hill plots. Anal Biochem. 1981; 114(2):322-9. DOI: 10.1016/0003-2697(81)90488-7. View

13.

Karim M, Lee H . Amperometric phenol biosensor based on covalent immobilization of tyrosinase on Au nanoparticle modified screen printed carbon electrodes. Talanta. 2013; 116:991-6. DOI: 10.1016/j.talanta.2013.08.003. View

14.

Chen Q, Kubo I . Kinetics of mushroom tyrosinase inhibition by quercetin. J Agric Food Chem. 2002; 50(14):4108-12. DOI: 10.1021/jf011378z. View

15.

Bayramoglu G, Akbulut A, Yakup Arica M . Immobilization of tyrosinase on modified diatom biosilica: enzymatic removal of phenolic compounds from aqueous solution. J Hazard Mater. 2012; 244-245:528-36. DOI: 10.1016/j.jhazmat.2012.10.041. View

16.

Ibarra-Escutia P, Gomez J, Calas-Blanchard C, Marty J, Ramirez-Silva M . Amperometric biosensor based on a high resolution photopolymer deposited onto a screen-printed electrode for phenolic compounds monitoring in tea infusions. Talanta. 2010; 81(4-5):1636-42. DOI: 10.1016/j.talanta.2010.03.017. View