Prognostic Significance of Epithelial-mesenchymal Transition-related Markers in Extrahepatic Cholangiocarcinoma: Comprehensive Immunohistochemical Study Using a Tissue Microarray

Overview

Journal Br J Cancer

Specialty Oncology

Date 2014 Aug 1

PMID 25077440

Citations 40

Authors

T Nitta

T Mitsuhashi

Y Hatanaka

M Miyamoto

K Oba

T Tsuchikawa

Y Suzuki

K C Hatanaka

S Hirano

Y Matsuno

Affiliations

Soon will be listed here.

Abstract

Background: Epithelial-mesenchymal transition (EMT) is characterised by the loss of cell-to-cell adhesion and gaining of mesenchymal phenotypes. Epithelial-mesenchymal transition is proposed to occur in various developmental processes and cancer progression. 'Cadherin switch', a process in which cells shift to express different isoforms of the cadherin transmembrane protein and usually refers to a switch from the expression of E-cadherin to N-cadherin, is one aspect of EMT and can have a profound effect on tumour invasion/metastasis. The aim of this study was to investigate the clinicopathological significance of EMT-related proteins and cadherin switch in extrahepatic cholangiocarcinoma (EHCC).

Methods: We investigated the association between altered expression of 12 EMT-related proteins and clinical outcomes in patients with EHCC (n=117) using immunohistochemistry on tissue microarrays.

Results: Univariate and multivariate analyses revealed that, in addition to N classification (P=0.0420), the expression of E-cadherin (P=0.0208), N-cadherin (P=0.0038) and S100A4 (P=0.0157) was each an independent and a significant prognostic factor. We also demonstrated that cadherin switch was independently associated with poor prognosis (P=0.0143) in patients with EHCC.

Conclusions: These results may provide novel information for selection of patients with EHCC who require adjuvant therapy and strict surveillance.

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