Eosinophil Granule Proteins ECP and EPX As Markers for a Potential Early-stage Inflammatory Lesion in Female Genital Schistosomiasis (FGS)

Overview

Journal PLoS Negl Trop Dis

Specialties Infectious Diseases
Tropical Medicine

Date 2014 Jul 18

PMID 25033206

Citations 6

Authors

Charles Emile Ramarokoto

Anna Overgaard Kildemoes

Bodo Sahondra Randrianasolo

Pascaline Ravoniarimbinina

Vololomboahangy Elisabeth Ravaoalimalala

Peter Leutscher

Eyrun Floerecke Kjetland

Birgitte Jyding Vennervald

Affiliations

Soon will be listed here.

Abstract

Background: Genital granulomas induced by Schistosoma haematobium eggs can manifest as different lesion types visible by colposcopy; rubbery papules (RP), homogenous sandy patches (HSP) and grainy sandy patches (GSP). Pronounced tissue eosinophilia is a candidate marker for active S. haematobium pathology, as viable schistosome egg granulomas often are eosinophil rich. Here it was investigated whether eosinophil granule proteins ECP (eosinophil cationic protein) and EPX (eosinophil protein-X) in urine and genital lavage can be used as markers for active FGS lesions.

Methods: Uro-genital samples from 118 Malagasy women were analysed for ECP and EPX by standard sandwich avidin/biotin amplified ELISA.

Principal Findings: The women with RP lesions had significantly higher levels of ECP and EPX in both lavage and urine. Furthermore, women with RP lesions were significantly younger than those with GSP. This could indicate that RP lesions might be more recently established and thus represent an earlier inflammatory lesion stage.

Conclusion: ECP in genital lavage might be a future tool aiding the identification of FGS pathology at a stage where reversibility remains a possibility following praziquantel treatment.

Citing Articles

Knowledge, experiences, and practices of women affected by female genital schistosomiasis in rural Madagascar: A qualitative study on disease perception, health impairment and social impact.

Schuster A, Randrianasolo B, Rabozakandraina O, Ramarokoto C, Bronnum D, Feldmeier H PLoS Negl Trop Dis. 2022; 16(11):e0010901.

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Schistosomiasis Morbidity Hotspots: Roles of the Human Host, the Parasite and Their Interface in the Development of Severe Morbidity.

Mawa P, Kincaid-Smith J, Tukahebwa E, Webster J, Wilson S Front Immunol. 2021; 12:635869.

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Galectins, Eosinophiles, and Macrophages May Contribute to Egg-Induced Immunopathology in a Mouse Model.

Ye Z, Huang S, Zhang Y, Mei X, Zheng H, Li M Front Immunol. 2020; 11:146.

PMID: 32231658 PMC: 7082360. DOI: 10.3389/fimmu.2020.00146.

Female genital schistosomiasis and HIV/AIDS: Reversing the neglect of girls and women.

Hotez P, Harrison W, Fenwick A, Bustinduy A, Ducker C, Mbabazi P PLoS Negl Trop Dis. 2019; 13(4):e0007025.

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References

Jourdan P, Roald B, Poggensee G, Gundersen S, Kjetland E . Increased vascularity in cervicovaginal mucosa with Schistosoma haematobium infection. PLoS Negl Trop Dis. 2011; 5(6):e1170. PMC: 3110160. DOI: 10.1371/journal.pntd.0001170. View

Kjetland E, Ndhlovu P, Mduluza T, Gomo E, Gwanzura L, Mason P . Simple clinical manifestations of genital Schistosoma haematobium infection in rural Zimbabwean women. Am J Trop Med Hyg. 2005; 72(3):311-9. View

Dunnette S, Loegering D, Checkel J, Kita H, Thomas L, Gleich G . Eosinophil granule proteins in peripheral blood granulocytes. J Leukoc Biol. 1992; 52(6):611-8. DOI: 10.1002/jlb.52.6.611. View

Hatz C, Vennervald B, Nkulila T, Vounatsou P, Kombe Y, Mayombana C . Evolution of Schistosoma haematobium-related pathology over 24 months after treatment with praziquantel among school children in southeastern Tanzania. Am J Trop Med Hyg. 1998; 59(5):775-81. DOI: 10.4269/ajtmh.1998.59.775. View

Freedman D, Ottesen E . Eggs of Schistosoma mansoni stimulate endothelial cell proliferation in vitro. J Infect Dis. 1988; 158(3):556-62. DOI: 10.1093/infdis/158.3.556. View

van der Werf M, de Vlas S, Brooker S, Looman C, Nagelkerke N, Habbema J . Quantification of clinical morbidity associated with schistosome infection in sub-Saharan Africa. Acta Trop. 2003; 86(2-3):125-39. DOI: 10.1016/s0001-706x(03)00029-9. View

Peters M, Rodriguez M, Gleich G . Localization of human eosinophil granule major basic protein, eosinophil cationic protein, and eosinophil-derived neurotoxin by immunoelectron microscopy. Lab Invest. 1986; 54(6):656-62. View

Jourdan P, Holmen S, Gundersen S, Roald B, Kjetland E . HIV target cells in Schistosoma haematobium-infected female genital mucosa. Am J Trop Med Hyg. 2011; 85(6):1060-4. PMC: 3225152. DOI: 10.4269/ajtmh.2011.11-0135. View

Gelfand M, Ross C, Blair D, Castle W, Weber M . Schistosomiasis of the male pelvic organs. Severity of infection as determined by digestion of tissue and histologic methods in 300 cadavers. Am J Trop Med Hyg. 1970; 19(5):779-84. View

10.

Hotez P, Kamath A . Neglected tropical diseases in sub-saharan Africa: review of their prevalence, distribution, and disease burden. PLoS Negl Trop Dis. 2009; 3(8):e412. PMC: 2727001. DOI: 10.1371/journal.pntd.0000412. View

11.

King C, Dangerfield-Cha M . The unacknowledged impact of chronic schistosomiasis. Chronic Illn. 2008; 4(1):65-79. DOI: 10.1177/1742395307084407. View

12.

de Jonge N, Fillie Y, Hilberath G, Krijger F, Lengeler C, de Savigny D . Presence of the schistosome circulating anodic antigen (CAA) in urine of patients with Schistosoma mansoni or S. haematobium infections. Am J Trop Med Hyg. 1989; 41(5):563-9. DOI: 10.4269/ajtmh.1989.41.563. View

13.

Kjetland E, Ndhlovu P, Mduluza T, Deschoolmeester V, Midzi N, Gomo E . The effects of genital schistosoma haematobium on human papillomavirus and the development of cervical neoplasia after five years in a Zimbabwean population. Eur J Gynaecol Oncol. 2010; 31(2):169-73. View

14.

Khalaf I, Shokeir A, Shalaby M . Urologic complications of genitourinary schistosomiasis. World J Urol. 2011; 30(1):31-8. DOI: 10.1007/s00345-011-0751-7. View

15.

Talaat M, Watts S, Mekheimar S, Farook Ali H, Hamed H . The social context of reproductive health in an Egyptian hamlet: a pilot study to identify female genital schistosomiasis. Soc Sci Med. 2003; 58(3):515-24. DOI: 10.1016/j.socscimed.2003.09.018. View

16.

Kjetland E, Leutscher P, Ndhlovu P . A review of female genital schistosomiasis. Trends Parasitol. 2012; 28(2):58-65. DOI: 10.1016/j.pt.2011.10.008. View

17.

Swai B, Poggensee G, Mtweve S, Krantz I . Female genital schistosomiasis as an evidence of a neglected cause for reproductive ill-health: a retrospective histopathological study from Tanzania. BMC Infect Dis. 2006; 6:134. PMC: 1564144. DOI: 10.1186/1471-2334-6-134. View

18.

Tischendorf F, Brattig N, Burchard G, Kubica T, Kreuzpaintner G, Lintzel M . Eosinophils, eosinophil cationic protein and eosinophil-derived neurotoxin in serum and urine of patients with onchocerciasis coinfected with intestinal nematodes and in urinary schistosomiasis. Acta Trop. 1999; 72(2):157-73. DOI: 10.1016/s0001-706x(98)00088-6. View

19.

Sur S, Glitz D, Kita H, Kujawa S, Peterson E, Weiler D . Localization of eosinophil-derived neurotoxin and eosinophil cationic protein in neutrophilic leukocytes. J Leukoc Biol. 1998; 63(6):715-22. DOI: 10.1002/jlb.63.6.715. View

20.

Yirenya-Tawiah D, Amoah C, Apea-Kubi K, Dade M, Ackumey M, Annang T . A survey of female genital schistosomiasis of the lower reproductive tract in the volta basin of Ghana. Ghana Med J. 2011; 45(1):16-21. PMC: 3090093. DOI: 10.4314/gmj.v45i1.68917. View