Crystallization and Preliminary Analysis of the NqrA and NqrC Subunits of the Na+-translocating NADH:ubiquinone Oxidoreductase from Vibrio Cholerae

Overview

Journal Acta Crystallogr F Struct Biol Commun

Specialty Chemistry

Date 2014 Jul 10

PMID 25005105

Citations 7

Authors

Georg Vohl

Ruslan Nedielkov

Bjorn Claussen

Marco S Casutt

Thomas Vorburger

Kay Diederichs

Heiko M Moller

Julia Steuber

Gunter Fritz

Affiliations

Soon will be listed here.

Abstract

The Na+-translocating NADH:ubiquinone oxidoreductase (Na+-NQR) from Vibrio cholerae is a membrane protein complex consisting of six different subunits NqrA-NqrF. The major domains of the NqrA and NqrC subunits were heterologously expressed in Escherichia coli and crystallized. The structure of NqrA1-377 was solved in space groups C222₁ and P2₁ by SAD phasing and molecular replacement at 1.9 and 2.1 Å resolution, respectively. NqrC devoid of the transmembrane helix was co-expressed with ApbE to insert the flavin mononucleotide group covalently attached to Thr225. The structure was determined by molecular replacement using apo-NqrC of Parabacteroides distasonis as search model at 1.8 Å resolution.

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