Crystallization and Preliminary Crystallographic Analysis of Histamine Dehydrogenase from Natrinema Gari BCC 24369

Overview

Journal Acta Crystallogr F Struct Biol Commun

Specialty Chemistry

Date 2014 Jul 10

PMID 25005094

Authors

Dongwen Zhou

Wonnop Visessanguan

Siriporn Chaikaew

Soottawat Benjakul

Kohei Oda

Alexander Wlodawer

Affiliations

Soon will be listed here.

Abstract

Histamine dehydrogenase (HADH) catalyzes the oxidative deamination of histamine, resulting in the production of imidazole acetaldehyde and an ammonium ion. The enzyme isolated from the newly identified halophilic archaeon Natrinema gari BCC 24369 is significantly different from the previously described protein from Nocardioides simplex. This newly identified HADH comprises three subunits with molecular weights of 49.0, 24.7 and 23.9 kDa, respectively, and is optimally active under high-salt conditions (3.5-5 M NaCl). As a step in the exploration of the unique properties of the protein, the HADH heterotrimer was purified and crystallized. Crystals were obtained using the sitting-drop vapor-diffusion method from a solution composed of 0.2 M calcium chloride dihydrate, 0.1 M HEPES pH 7.5, 28% PEG 400. Diffraction data were collected at -173°C to a resolution limit of 2.4 Å on the Southeast Regional Collaborative Access Team (SER-CAT) beamline 22-ID at the Advanced Photon Source, Argonne National Laboratory. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a=211.9, b=58.6, c=135.4 Å, β=103.0°. The estimated Matthews coefficient is 3.21 Å3 Da(-1), corresponding to 61.7% solvent content.

References

Broide D, Gleich G, Cuomo A, Coburn D, Federman E, Schwartz L . Evidence of ongoing mast cell and eosinophil degranulation in symptomatic asthma airway. J Allergy Clin Immunol. 1991; 88(4):637-48. DOI: 10.1016/0091-6749(91)90158-k. View

Tuomisto L, Kilpelainen H, RIEKKINEN P . Histamine and histamine-N-methyltransferase in the CSF of patients with multiple sclerosis. Agents Actions. 1983; 13(2-3):255-7. DOI: 10.1007/BF01967346. View

Liu M, Bleecker E, Lichtenstein L, Niv Y, McLemore T, PERMUTT S . Evidence for elevated levels of histamine, prostaglandin D2, and other bronchoconstricting prostaglandins in the airways of subjects with mild asthma. Am Rev Respir Dis. 1990; 142(1):126-32. DOI: 10.1164/ajrccm/142.1.126. View

Bakke M, Sato T, Ichikawa K, Nishimura I . Histamine dehydrogenase from Rhizobium sp.: gene cloning, expression in Escherichia coli, characterization and application to histamine determination. J Biotechnol. 2005; 119(3):260-71. DOI: 10.1016/j.jbiotec.2005.04.005. View

Juhlin L . Localization and content of histamine in normal and diseased skin. Acta Derm Venereol. 1967; 47(6):383-91. View

Wardlaw A, Dunnette S, Gleich G, Collins J, Kay A . Eosinophils and mast cells in bronchoalveolar lavage in subjects with mild asthma. Relationship to bronchial hyperreactivity. Am Rev Respir Dis. 1988; 137(1):62-9. DOI: 10.1164/ajrccm/137.1.62. View

Frewin D, Cleland L, Jonsson J, Robertson P . Histamine levels in human synovial fluid. J Rheumatol. 1986; 13(1):13-4. View

Fujieda N, Tsuse N, Satoh A, Ikeda T, Kano K . Production of completely flavinylated histamine dehydrogenase, unique covalently bound flavin, and iron-sulfur cluster-containing enzyme of nocardioides simplex in Escherichia coli, and its properties. Biosci Biotechnol Biochem. 2005; 69(12):2459-62. DOI: 10.1271/bbb.69.2459. View

Kaplan A, Horakova Z, Katz S . Assessment of tissue fluid histamine levels in patients with urticaria. J Allergy Clin Immunol. 1978; 61(6):350-4. DOI: 10.1016/0091-6749(78)90113-6. View

10.

Limburg J, Mure M, Klinman J . Cloning and characterization of histamine dehydrogenase from Nocardioides simplex. Arch Biochem Biophys. 2005; 436(1):8-22. DOI: 10.1016/j.abb.2004.11.024. View

11.

Petersen L, Hansen U, Kristensen J, Nielsen H, Skov P, Nielsen H . Studies on mast cells and histamine release in psoriasis: the effect of ranitidine. Acta Derm Venereol. 1998; 78(3):190-3. DOI: 10.1080/000155598441503. View

12.

Schagger H . Tricine-SDS-PAGE. Nat Protoc. 2007; 1(1):16-22. DOI: 10.1038/nprot.2006.4. View

13.

Jarjour N, Calhoun W, Schwartz L, Busse W . Elevated bronchoalveolar lavage fluid histamine levels in allergic asthmatics are associated with increased airway obstruction. Am Rev Respir Dis. 1991; 144(1):83-7. DOI: 10.1164/ajrccm/144.1.83. View

14.

Dietmann S, Holm L . Identification of homology in protein structure classification. Nat Struct Biol. 2001; 8(11):953-7. DOI: 10.1038/nsb1101-953. View

15.

Greaves M, Sondergaard J . Urticaria pigmentosa and factitious urticaria. Direct evidence for release of histamine and other smooth muscle-contracting agents in dermographic skin. Arch Dermatol. 1970; 101(4):418-25. DOI: 10.1001/archderm.101.4.418. View

16.

Crisp A . Studies of histamine in the rheumatoid joint. Rheumatol Int. 1984; 4(3):125-8. DOI: 10.1007/BF00541181. View

17.

Kabsch W . XDS. Acta Crystallogr D Biol Crystallogr. 2010; 66(Pt 2):125-32. PMC: 2815665. DOI: 10.1107/S0907444909047337. View

18.

Siddiqui J, Shoeb S, Takayama S, Shimizu E, Yorifuji T . Purification and characterization of histamine dehydrogenase from Nocardioides simplex IFO 12069. FEMS Microbiol Lett. 2000; 189(2):183-7. DOI: 10.1111/j.1574-6968.2000.tb09227.x. View

19.

Wenzel S, Fowler 3rd A, Schwartz L . Activation of pulmonary mast cells by bronchoalveolar allergen challenge. In vivo release of histamine and tryptase in atopic subjects with and without asthma. Am Rev Respir Dis. 1988; 137(5):1002-8. DOI: 10.1164/ajrccm/137.5.1002. View

20.

Tapingkae W, Tanasupawat S, Itoh T, Parkin K, Benjakul S, Visessanguan W . Natrinema gari sp. nov., a halophilic archaeon isolated from fish sauce in Thailand. Int J Syst Evol Microbiol. 2008; 58(Pt 10):2378-83. DOI: 10.1099/ijs.0.65644-0. View