Effects of Ketogenic Diets on the Occurrence of Pilocarpine-induced Status Epilepticus of Rats

Overview

Journal Metab Brain Dis

Publisher Springer

Specialties Endocrinology
Neurology

Date 2014 Jul 10

PMID 25005004

Citations 5

Authors

Iclea Rocha Gama

Euclides Marinho Trindade-Filho

Suzana Lima Oliveira

Nassib Bezerra Bueno

Isabelle Tenorio Melo

Cyro Rego Cabral-Junior

Elenita M Barros

Jaqueline A Galvao

Wanessa S Pereira

Raphaela C Ferreira

Bruna R Domingos

Terezinha da Rocha Ataide

Affiliations

Soon will be listed here.

Abstract

Two sources of medium-chain triglycerides--triheptanoin with anaplerotic properties and coconut oil with antioxidant features--have emerged as promising therapeutic options for the management of pharmacoresistant epilepsy. We investigated the effects of ketogenic diets (KDs) containing coconut oil, triheptanoin, or soybean oil on pilocarpine-induced status epilepticus (SE) in rats. Twenty-four adult male Wistar rats were divided into 4 groups and fed a control diet (7% lipids) or a KD containing soybean oil, coconut oil, or triheptanoin (69.8% lipids). The ketogenic and control diets had a lipid:carbohydrate + protein ratio of 1:11.8 and 3.5:1, respectively. SE was induced in all rats 20 days after initiation of the dietary treatment, through the administration of pilocarpine (340 mg/kg; i.p.). The latency, frequency, duration, and severity of seizures before and during SE were observed with a camcorder. SE was aborted after 3 h with the application of diazepam (5 mg/kg; i.p.). The rats in the triheptanoin-based KD group needed to undergo a higher number of seizures to develop SE, as compared to the control group (P < 0.05). Total weight gain, intake, energy intake, and feed efficiency coefficient, prior to induction of SE, differed between groups (P < 0.05), where the triheptanoin-based KD group showed less weight gain than all other groups, less energy intake than the Control group and intermediate values of feed efficiency coefficient between Control and other KDs groups. Triheptanoin-based KD may have a neuroprotective effect on the establishment of SE in Wistar rats.

Citing Articles

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Triheptanoin alters [U-C]-glucose incorporation into glycolytic intermediates and increases TCA cycling by normalizing the activities of pyruvate dehydrogenase and oxoglutarate dehydrogenase in a chronic epilepsy mouse model.

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Anticonvulsant effect of exogenous β-hydroxybutyrate on kainic acid-induced epilepsy.

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Ketogenic diet prevents epileptogenesis and disease progression in adult mice and rats.

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