Alpha 2.0
Login Register
» Articles » PMID: 2499657

Circulating Interleukin 6 During a Continuous Infusion of Tumor Necrosis Factor and Interferon Gamma

Overview
Journal J Exp Med
Specialties Allergy & Immunology
General Medicine
Date 1989 Jun 1
PMID 2499657
Citations 34
Authors
P Brouckaert
D R Spriggs
G Demetri
D W Kufe
W Fiers
Affiliations
Soon will be listed here.
Abstract

Plasma samples obtained from patients receiving a 24-h continuous infusion of human recombinant (hr)TNF or a combination of two overlapping, 24-h continuous infusions of hrIFN-gamma and hrTNF were analyzed for IL-6 in a sensitive bioassay. A transient appearance of circulating IL-6 was observed with peak levels between 3 and 6 h after the start of the hrTNF infusion. These peak levels correlated quite well with the dose of hrTNF administered (r = 0.86; p less than 0.001). The maximal value observed was 27.5 ng/ml IL-6 in a sample of a patient receiving 545 micrograms/m2 hrTNF. The combination of hrIFN-gamma (200 micrograms/m2) and hrTNF in the infusions resulted in higher IL-6 levels than a comparable dose of hrTNF alone. A maximal value of 23.5 ng/ml IL-6 was observed in a patient receiving 205 micrograms/m2 hrTNF. No IL-6 was found in the plasma of patients during the 12-h infusion with hrIFN-gamma alone, except for two borderline samples.

Citing Articles

Impact of Injury Severity on the Inflammatory State and Severe Anemia.

Apple C, Miller E, Loftus T, Kannan K, Parvataneni H, Hagen J J Surg Res. 2019; 248:109-116.

PMID: 31881381 PMC: 7054167. DOI: 10.1016/j.jss.2019.10.046.

How cytokine networks fuel inflammation: Toward a cytokine-based disease taxonomy.

Schett G, Elewaut D, McInnes I, Dayer J, Neurath M Nat Med. 2013; 19(7):822-4.

PMID: 23836224 DOI: 10.1038/nm.3260.

Ablation of TNF-RI/RII expression in Alzheimer's disease mice leads to an unexpected enhancement of pathology: implications for chronic pan-TNF-α suppressive therapeutic strategies in the brain.

Montgomery S, Mastrangelo M, Habib D, Narrow W, Knowlden S, Wright T Am J Pathol. 2011; 179(4):2053-70.

PMID: 21835156 PMC: 3181376. DOI: 10.1016/j.ajpath.2011.07.001.

Induction of circulating phospholipase A(2) by intravenous administration of recombinant human tumour necrosis factor.

Pruzanski W, Sherman M, Kufe D, Vadas P Mediators Inflamm. 1992; 1(4):235-40.

PMID: 18475466 PMC: 2365345. DOI: 10.1155/S0962935192000358.

A mediator role for metallothionein in tumor necrosis factor-induced lethal shock.

Waelput W, Broekaert D, Vandekerckhove J, Brouckaert P, Tavernier J, Libert C J Exp Med. 2001; 194(11):1617-24.

PMID: 11733576 PMC: 2193525. DOI: 10.1084/jem.194.11.1617.

References
1.
Landegren U . Measurement of cell numbers by means of the endogenous enzyme hexosaminidase. Applications to detection of lymphokines and cell surface antigens. J Immunol Methods. 1984; 67(2):379-88. DOI: 10.1016/0022-1759(84)90477-0. View
2.
Brouckaert P, Leroux-Roels G, Guisez Y, Tavernier J, Fiers W . In vivo anti-tumour activity of recombinant human and murine TNF, alone and in combination with murine IFN-gamma, on a syngeneic murine melanoma. Int J Cancer. 1986; 38(5):763-9. DOI: 10.1002/ijc.2910380521. View
3.
Van Snick J, Cayphas S, Vink A, Uyttenhove C, Coulie P, Rubira M . Purification and NH2-terminal amino acid sequence of a T-cell-derived lymphokine with growth factor activity for B-cell hybridomas. Proc Natl Acad Sci U S A. 1986; 83(24):9679-83. PMC: 387204. DOI: 10.1073/pnas.83.24.9679. View
4.
Tracey K, Lowry S, Cerami A . Cachectin: a hormone that triggers acute shock and chronic cachexia. J Infect Dis. 1988; 157(3):413-20. DOI: 10.1093/infdis/157.3.413. View
5.
Wong G, Clark S . Multiple actions of interleukin 6 within a cytokine network. Immunol Today. 1988; 9(5):137-9. DOI: 10.1016/0167-5699(88)91200-5. View