The Involvement of MiR-23a/APAF1 Regulation Axis in Colorectal Cancer

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2014 Jul 4

PMID 24992592

Citations 13

Authors

Fung Lin Yong

Chee Woon Wang

April Camilla Roslani

Chee Wei Law

Affiliations

Soon will be listed here.

Abstract

Recent advances in microRNAome have made microRNAs (miRNAs) a compelling novel class of biomarker in cancer biology. In the present study, the role of miR-23a in the carcinogenesis of colorectal cancer (CRC) was investigated. Cell viability, apoptosis, and caspase 3/7 activation analyses were conducted to determine the potentiality of apoptosis resistance function of miR-23a in CRC. Luciferase assay was performed to verify a putative target site of miR-23a in the 3'-UTR of apoptosis protease activating factor 1 (APAF1) mRNA. The expression levels of miR-23a and APAF1 in CRC cell lines (SW480 and SW620) and clinical samples were assessed using reverse transcription-quantitative real-time PCR (RT-qPCR) and Western blot. We found that the inhibition of miR-23a in SW480 and SW620 cell lines resulted in significant reduction of cell viability and promotion of cell apoptosis. Moreover, miR-23a up-regulation was coupled with APAF1 down-regulation in CRC tissue samples. Taken together, miR-23a was identified to regulate apoptosis in CRC. Our study highlights the potential application of miR-23a/APAF1 regulation axis in miRNA-based therapy and prognostication.

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References

Krek A, Grun D, Poy M, Wolf R, Rosenberg L, Epstein E . Combinatorial microRNA target predictions. Nat Genet. 2005; 37(5):495-500. DOI: 10.1038/ng1536. View

Doench J, Sharp P . Specificity of microRNA target selection in translational repression. Genes Dev. 2004; 18(5):504-11. PMC: 374233. DOI: 10.1101/gad.1184404. View

Asangani I, Rasheed S, Nikolova D, Leupold J, Colburn N, Post S . MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer. Oncogene. 2007; 27(15):2128-36. DOI: 10.1038/sj.onc.1210856. View

Yamamichi N, Shimomura R, Inada K, Sakurai K, Haraguchi T, Ozaki Y . Locked nucleic acid in situ hybridization analysis of miR-21 expression during colorectal cancer development. Clin Cancer Res. 2009; 15(12):4009-16. DOI: 10.1158/1078-0432.CCR-08-3257. View

Leibovitz A, Stinson J, McCombs 3rd W, McCoy C, Mazur K, Mabry N . Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 1976; 36(12):4562-9. View

Chan S, Slack F . microRNA-mediated silencing inside P-bodies. RNA Biol. 2006; 3(3):97-100. DOI: 10.4161/rna.3.3.3499. View

Bartel D . MicroRNAs: target recognition and regulatory functions. Cell. 2009; 136(2):215-33. PMC: 3794896. DOI: 10.1016/j.cell.2009.01.002. View

Huerta S, Heinzerling J, Anguiano-Hernandez Y, Huerta-Yepez S, Lin J, Chen D . Modification of gene products involved in resistance to apoptosis in metastatic colon cancer cells: roles of Fas, Apaf-1, NFkappaB, IAPs, Smac/DIABLO, and AIF. J Surg Res. 2007; 142(1):184-94. DOI: 10.1016/j.jss.2006.12.551. View

Tsuchida A, Ohno S, Wu W, Borjigin N, Fujita K, Aoki T . miR-92 is a key oncogenic component of the miR-17-92 cluster in colon cancer. Cancer Sci. 2011; 102(12):2264-71. DOI: 10.1111/j.1349-7006.2011.02081.x. View

10.

Rodrigues N, Rowan A, Smith M, Kerr I, Bodmer W, Gannon J . p53 mutations in colorectal cancer. Proc Natl Acad Sci U S A. 1990; 87(19):7555-9. PMC: 54786. DOI: 10.1073/pnas.87.19.7555. View

11.

Lynam-Lennon N, Maher S, Reynolds J . The roles of microRNA in cancer and apoptosis. Biol Rev Camb Philos Soc. 2008; 84(1):55-71. DOI: 10.1111/j.1469-185X.2008.00061.x. View

12.

Wang N, Zhu M, Tsao S, Man K, Zhang Z, Feng Y . MiR-23a-mediated inhibition of topoisomerase 1 expression potentiates cell response to etoposide in human hepatocellular carcinoma. Mol Cancer. 2013; 12(1):119. PMC: 3856574. DOI: 10.1186/1476-4598-12-119. View

13.

Li B, Sun M, Gao F, Liu W, Yang Y, Liu H . Up-regulated expression of miR-23a/b targeted the pro-apoptotic Fas in radiation-induced thymic lymphoma. Cell Physiol Biochem. 2013; 32(6):1729-40. DOI: 10.1159/000356607. View

14.

Robles A, Bemmels N, Foraker A, Harris C . APAF-1 is a transcriptional target of p53 in DNA damage-induced apoptosis. Cancer Res. 2001; 61(18):6660-4. View

15.

Paik S, Jang K, Song Y, Jang S, Min K, Han H . Reduced expression of Apaf-1 in colorectal adenocarcinoma correlates with tumor progression and aggressive phenotype. Ann Surg Oncol. 2007; 14(12):3453-9. DOI: 10.1245/s10434-007-9541-2. View

16.

Dweep H, Sticht C, Pandey P, Gretz N . miRWalk--database: prediction of possible miRNA binding sites by "walking" the genes of three genomes. J Biomed Inform. 2011; 44(5):839-47. DOI: 10.1016/j.jbi.2011.05.002. View

17.

Meltzer P . Cancer genomics: small RNAs with big impacts. Nature. 2005; 435(7043):745-6. DOI: 10.1038/435745a. View

18.

Campioni M, Santini D, Tonini G, Murace R, Dragonetti E, Spugnini E . Role of Apaf-1, a key regulator of apoptosis, in melanoma progression and chemoresistance. Exp Dermatol. 2005; 14(11):811-8. DOI: 10.1111/j.1600-0625.2005.00360.x. View

19.

Bray F, Jemal A, Grey N, Ferlay J, Forman D . Global cancer transitions according to the Human Development Index (2008-2030): a population-based study. Lancet Oncol. 2012; 13(8):790-801. DOI: 10.1016/S1470-2045(12)70211-5. View

20.

Yuan J, Reed A, Chen F, Stewart Jr C . Statistical analysis of real-time PCR data. BMC Bioinformatics. 2006; 7:85. PMC: 1395339. DOI: 10.1186/1471-2105-7-85. View