Bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting MDFIC Gene

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2020 Oct 23

PMID 33092227

Citations 2

Authors

Xin Hu

Yishen Xing

Ling Ren

Yahui Wang

Qian Li

Qiyuan Yang

Min Du

Lingyang Xu

Luc Willems

Junya Li

Lupei Zhang

Affiliations

Soon will be listed here.

Abstract

miR-23a, a member of the miR-23a/24-2/27a cluster, has been demonstrated to play pivotal roles in many cellular activities. However, the mechanisms of how bta-miR-23a controls the myogenic differentiation (MD) of PDGFRα bovine progenitor cells (bPCs) remain poorly understood. In the present work, bta-miR-23a expression was increased during the MD of bPCs. Moreover, bta-miR-23a overexpression significantly promoted the MD of bPCs. Luciferase reporter assays showed that the 3'-UTR region of (MyoD family inhibitor domain containing) could be a promising target of bta-miR-23a, which resulted in its post-transcriptional down-regulation. Additionally, the knockdown of by siRNA facilitated the MD of bPCs, while the overexpression of inhibited the activating effect of bta-miR-23a during MD. Of note, might function through the interaction between transcription factor and promoter. This study reveals that bta-miR-23a can promote the MD of bPCs through post-transcriptional downregulation of .

Citing Articles

Genome-based analysis of the genetic pattern of black sheep in Qira sheep.

Zhou W, Li X, Zhang X, Zhu L, Peng Y, Zhang C BMC Genomics. 2025; 26(1):114.

PMID: 39915708 PMC: 11800528. DOI: 10.1186/s12864-025-11233-5.

The Function and Regulation Mechanism of Non-Coding RNAs in Muscle Development.

Yang Y, Wu J, Liu W, Zhao Y, Chen H Int J Mol Sci. 2023; 24(19).

PMID: 37833983 PMC: 10572267. DOI: 10.3390/ijms241914534.

Utilizing Variants Identified with Multiple Genome-Wide Association Study Methods Optimizes Genomic Selection for Growth Traits in Pigs.

Zhang R, Zhang Y, Liu T, Jiang B, Li Z, Qu Y Animals (Basel). 2023; 13(4).

PMID: 36830509 PMC: 9952664. DOI: 10.3390/ani13040722.

References

Guan L, Hu X, Liu L, Xing Y, Zhou Z, Liang X . bta-miR-23a involves in adipogenesis of progenitor cells derived from fetal bovine skeletal muscle. Sci Rep. 2017; 7:43716. PMC: 5334644. DOI: 10.1038/srep43716. View

KRAUT N, Snider L, Chen C, Tapscott S, Groudine M . Requirement of the mouse I-mfa gene for placental development and skeletal patterning. EMBO J. 1998; 17(21):6276-88. PMC: 1170953. DOI: 10.1093/emboj/17.21.6276. View

Hadjimichael C, Nikolaou C, Papamatheakis J, Kretsovali A . MicroRNAs for Fine-Tuning of Mouse Embryonic Stem Cell Fate Decision through Regulation of TGF-β Signaling. Stem Cell Reports. 2016; 6(3):292-301. PMC: 4788761. DOI: 10.1016/j.stemcr.2016.01.004. View

Chen B, Zhu A, Tian L, Xin Y, Liu X, Peng Y . miR‑23a suppresses pancreatic cancer cell progression by inhibiting PLK‑1 expression. Mol Med Rep. 2018; 18(1):105-112. PMC: 6059658. DOI: 10.3892/mmr.2018.8941. View

Thebault S, Gachon F, Lemasson I, Devaux C, Mesnard J . Molecular cloning of a novel human I-mfa domain-containing protein that differently regulates human T-cell leukemia virus type I and HIV-1 expression. J Biol Chem. 2000; 275(7):4848-57. DOI: 10.1074/jbc.275.7.4848. View

Wang L, Chen X, Zheng Y, Li F, Lu Z, Chen C . MiR-23a inhibits myogenic differentiation through down regulation of fast myosin heavy chain isoforms. Exp Cell Res. 2012; 318(18):2324-34. DOI: 10.1016/j.yexcr.2012.06.018. View

Zhang L, Zhou X, Michal J, Ding B, Li R, Jiang Z . Genome wide screening of candidate genes for improving piglet birth weight using high and low estimated breeding value populations. Int J Biol Sci. 2014; 10(3):236-44. PMC: 3957079. DOI: 10.7150/ijbs.7744. View

Wada S, Kato Y, Okutsu M, Miyaki S, Suzuki K, Yan Z . Translational suppression of atrophic regulators by microRNA-23a integrates resistance to skeletal muscle atrophy. J Biol Chem. 2011; 286(44):38456-38465. PMC: 3207415. DOI: 10.1074/jbc.M111.271270. View

Hassan M, Gordon J, Beloti M, Croce C, van Wijnen A, Stein J . A network connecting Runx2, SATB2, and the miR-23a~27a~24-2 cluster regulates the osteoblast differentiation program. Proc Natl Acad Sci U S A. 2010; 107(46):19879-84. PMC: 2993380. DOI: 10.1073/pnas.1007698107. View

10.

Wu J, He D, Yue B, Zhang C, Fang X, Chen H . miR-101-1 expression pattern in Qinchuan cattle and its role in the regulation of cell differentiation. Gene. 2017; 636:64-69. DOI: 10.1016/j.gene.2017.09.026. View

11.

Wang Y, Schnegelsberg P, Dausman J, Jaenisch R . Functional redundancy of the muscle-specific transcription factors Myf5 and myogenin. Nature. 1996; 379(6568):823-5. DOI: 10.1038/379823a0. View

12.

Lin Z, Murtaza I, Wang K, Jiao J, Gao J, Li P . miR-23a functions downstream of NFATc3 to regulate cardiac hypertrophy. Proc Natl Acad Sci U S A. 2009; 106(29):12103-8. PMC: 2715539. DOI: 10.1073/pnas.0811371106. View

13.

Zhang W, Sun X, Tong H, Wang Y, Li S, Yan Y . Effect of differentiation on microRNA expression in bovine skeletal muscle satellite cells by deep sequencing. Cell Mol Biol Lett. 2017; 21:8. PMC: 5415838. DOI: 10.1186/s11658-016-0009-x. View

14.

Cossu G, Borello U . Wnt signaling and the activation of myogenesis in mammals. EMBO J. 1999; 18(24):6867-72. PMC: 1171749. DOI: 10.1093/emboj/18.24.6867. View

15.

Zhuang R, Bai X, Liu W . MicroRNA-23a depletion promotes apoptosis of ovarian cancer stem cell and inhibits cell migration by targeting DLG2. Cancer Biol Ther. 2019; 20(6):897-911. PMC: 6605979. DOI: 10.1080/15384047.2019.1579960. View

16.

Pan W, Jia Y, Huang T, Wang J, Tao D, Gan X . Beta-catenin relieves I-mfa-mediated suppression of LEF-1 in mammalian cells. J Cell Sci. 2006; 119(Pt 23):4850-6. DOI: 10.1242/jcs.03257. View

17.

Mercatelli N, Fittipaldi S, De Paola E, Dimauro I, Paronetto M, Jackson M . MiR-23-TrxR1 as a novel molecular axis in skeletal muscle differentiation. Sci Rep. 2017; 7(1):7219. PMC: 5543121. DOI: 10.1038/s41598-017-07575-0. View

18.

Du M, Tong J, Zhao J, Underwood K, Zhu M, Ford S . Fetal programming of skeletal muscle development in ruminant animals. J Anim Sci. 2009; 88(13 Suppl):E51-60. DOI: 10.2527/jas.2009-2311. View

19.

Snider L, Thirlwell H, Miller J, Moon R, Groudine M, Tapscott S . Inhibition of Tcf3 binding by I-mfa domain proteins. Mol Cell Biol. 2001; 21(5):1866-73. PMC: 86756. DOI: 10.1128/MCB.21.5.1866-1873.2001. View

20.

Wang Y, Ding X, Dai Y, Liu X, Guo H, Zhang Y . Identification and bioinformatics analysis of miRNAs involved in bovine skeletal muscle satellite cell myogenic differentiation. Mol Cell Biochem. 2015; 404(1-2):113-22. DOI: 10.1007/s11010-015-2371-9. View