Stepwise Splitting of Ribosomal Proteins from Yeast Ribosomes by LiCl

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 Jul 4

PMID 24991888

Citations 6

Authors

Kerli Piir

Tiina Tamm

Ivan Kisly

Triin Tammsalu

Jaanus Remme

Affiliations

Soon will be listed here.

Abstract

Structural studies have revealed that the core of the ribosome structure is conserved among ribosomes of all kingdoms. Kingdom-specific ribosomal proteins (r-proteins) are located in peripheral parts of the ribosome. In this work, the interactions between rRNA and r-proteins of eukaryote Saccharomyces cerevisiae ribosome were investigated applying LiCl induced splitting and quantitative mass spectrometry. R-proteins were divided into four groups according to their binding properties to the rRNA. Most yeast r-proteins are removed from rRNA by 0.5-1 M LiCl. Eukaryote-specific r-proteins are among the first to dissociate. The majority of the strong binders are known to be required for the early ribosome assembly events. As compared to the bacterial ribosome, yeast r-proteins are dissociated from rRNA at lower ionic strength. Our results demonstrate that the nature of protein-RNA interactions in the ribosome is not conserved between different kingdoms.

Citing Articles

Loss of Conserved rRNA Modifications in the Peptidyl Transferase Center Leads to Diminished Protein Synthesis and Cell Growth in Budding Yeast.

Leppik M, Pomerants L, Poldes A, Mihkelson P, Remme J, Tamm T Int J Mol Sci. 2024; 25(10).

PMID: 38791231 PMC: 11121408. DOI: 10.3390/ijms25105194.

Tau protein- induced sequestration of the eukaryotic ribosome: Implications in neurodegenerative disease.

Banerjee S, Ferdosh S, Nath Ghosh A, Barat C Sci Rep. 2020; 10(1):5225.

PMID: 32251304 PMC: 7090008. DOI: 10.1038/s41598-020-61777-7.

Functional Interactions of Ribosomal Intersubunit Bridges in .

Tamm T, Kisly I, Remme J Genetics. 2019; 213(4):1329-1339.

PMID: 31649153 PMC: 6893367. DOI: 10.1534/genetics.119.302777.

Ribosomal protein eL24, involved in two intersubunit bridges, stimulates translation initiation and elongation.

Kisly I, Remme J, Tamm T Nucleic Acids Res. 2018; 47(1):406-420.

PMID: 30407570 PMC: 6326817. DOI: 10.1093/nar/gky1083.

High-throughput RNA structure probing reveals critical folding events during early 60S ribosome assembly in yeast.

Burlacu E, Lackmann F, Aguilar L, Belikov S, van Nues R, Trahan C Nat Commun. 2017; 8(1):714.

PMID: 28959008 PMC: 5620067. DOI: 10.1038/s41467-017-00761-8.

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