Localization of Eukaryote-specific Ribosomal Proteins in a 5.5-Å Cryo-EM Map of the 80S Eukaryotic Ribosome

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2010 Oct 27

PMID 20974910

Citations 77

Authors

Jean-Paul Armache

Alexander Jarasch

Andreas M Anger

Elizabeth Villa

Thomas Becker

Shashi Bhushan

Fabrice Jossinet

Michael Habeck

Gulcin Dindar

Sibylle Franckenberg

Viter Marquez

Thorsten Mielke

Michael Thomm

Otto Berninghausen

Birgitta Beatrix

Johannes Soding

Eric Westhof

Daniel N Wilson

Roland Beckmann

Affiliations

Soon will be listed here.

Abstract

Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectron microscopy reconstructions of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution, together with a 6.1-Å map of a translating Saccharomyces cerevisiae 80S ribosome, we have localized and modeled 74/80 (92.5%) of the ribosomal proteins, encompassing 12 archaeal/eukaryote-specific small subunit proteins as well as the complete complement of the ribosomal proteins of the eukaryotic large subunit. Near-complete atomic models of the 80S ribosome provide insights into the structure, function, and evolution of the eukaryotic translational apparatus.

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