The Antibacterial Assay of Tectorigenin with Detergents or ATPase Inhibitors Against Methicillin-Resistant Staphylococcus Aureus

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2014 Jul 3

PMID 24987433

Citations 9

Authors

Dae-Ki Joung

Su-Hyun Mun

Kuang-Shim Lee

Ok-Hwa Kang

Jang-Gi Choi

Sung-Bae Kim

Ryong Gong

Myong-Soo Chong

Youn-Chul Kim

Dong-Sung Lee

Dong-Won Shin

Dong-Yeul Kwon

Affiliations

Soon will be listed here.

Abstract

Tectorigenin (TTR) is an O-methylated isoflavone derived from the rhizome of Belamacanda chinensis (L.) DC. It is known to perform a wide spectrum of biological activities such as antioxidant, anti-inflammatory, anti-tumor. The aim of this study is to examine the mechanism of antibacterial activity of TTR against methicillin-resistant Staphylococcus aureus (MRSA). The anti-MRSA activity of TTR was analyzed in combination assays with detergent, ATPase inhibitors, and peptidoglycan (PGN) derived from S. aureus. Transmission electron microscopy (TEM) was used to monitor survival characteristics and changes in S. aureus morphology. The MIC values of TTR against all the tested strains were 125 μ g/mL. The OD(600) of each suspension treated with a combination of Triton X-100, DCCD, and NaN3 with TTR (1/10 × MIC) had been reduced from 68% to 80%, compared to the TTR alone. At a concentration of 125 μ g/mL, PGN blocked antibacterial activity of TTR. This study indicates that anti-MRSA action of TTR is closely related to cytoplasmic membrane permeability and ABC transporter, and PGN at 125 μ g/mL directly bind to and inhibit TTR at 62.5 μ g/mL. These results can be important indication in study on antimicrobial activity mechanism against multidrug resistant strains.

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