Colistin Heteroresistance in Enterobacter Cloacae is Associated with Cross-resistance to the Host Antimicrobial Lysozyme

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2014 Jul 2

PMID 24982068

Citations 40

Authors

Brooke A Napier

Victor Band

Eileen M Burd

David S Weiss

Affiliations

Soon will be listed here.

Abstract

Here, we describe the first identification of colistin-heteroresistant Enterobacter cloacae in the United States. Treatment of this isolate with colistin increased the frequency of the resistant subpopulation and induced cross-resistance to the host antimicrobial lysozyme. This is the first description of heteroresistance conferring cross-resistance to a host antimicrobial and suggests that clinical treatment with colistin may inadvertently select for bacteria that are resistant to components of the host innate immune system.

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References

Mezzatesta M, Gona F, Stefani S . Enterobacter cloacae complex: clinical impact and emerging antibiotic resistance. Future Microbiol. 2012; 7(7):887-902. DOI: 10.2217/fmb.12.61. View

Zavascki A, Goldani L, Li J, Nation R . Polymyxin B for the treatment of multidrug-resistant pathogens: a critical review. J Antimicrob Chemother. 2007; 60(6):1206-15. DOI: 10.1093/jac/dkm357. View

Gaston M . Enterobacter: an emerging nosocomial pathogen. J Hosp Infect. 1988; 11(3):197-208. DOI: 10.1016/0195-6701(88)90098-9. View

Keller R, Pedroso M, Ritchmann R, Silva R . Occurrence of virulence-associated properties in Enterobacter cloacae. Infect Immun. 1998; 66(2):645-9. PMC: 113501. DOI: 10.1128/IAI.66.2.645-649.1998. View

Napier B, Burd E, Satola S, Cagle S, Ray S, McGann P . Clinical use of colistin induces cross-resistance to host antimicrobials in Acinetobacter baumannii. mBio. 2013; 4(3):e00021-13. PMC: 3663567. DOI: 10.1128/mBio.00021-13. View

Stock I, Gruger T, Wiedemann B . Natural antibiotic susceptibility of strains of the Enterobacter cloacae complex. Int J Antimicrob Agents. 2001; 18(6):537-45. DOI: 10.1016/s0924-8579(01)00463-0. View

Deresinski S . Vancomycin heteroresistance and methicillin-resistant Staphylococcus aureus. J Infect Dis. 2009; 199(5):605-9. DOI: 10.1086/596630. View

Laible N, Germaine G . Bactericidal activity of human lysozyme, muramidase-inactive lysozyme, and cationic polypeptides against Streptococcus sanguis and Streptococcus faecalis: inhibition by chitin oligosaccharides. Infect Immun. 1985; 48(3):720-8. PMC: 261241. DOI: 10.1128/iai.48.3.720-728.1985. View

Biswas S, Brunel J, Dubus J, Reynaud-Gaubert M, Rolain J . Colistin: an update on the antibiotic of the 21st century. Expert Rev Anti Infect Ther. 2012; 10(8):917-34. DOI: 10.1586/eri.12.78. View

10.

John Jr J, Sharbaugh R, BANNISTER E . Enterobacter cloacae: bacteremia, epidemiology, and antibiotic resistance. Rev Infect Dis. 1982; 4(1):13-28. DOI: 10.1093/clinids/4.1.13. View

11.

Tamayo M, Santiso R, Otero F, Bou G, Lepe J, McConnell M . Rapid determination of colistin resistance in clinical strains of Acinetobacter baumannii by use of the micromax assay. J Clin Microbiol. 2013; 51(11):3675-82. PMC: 3889750. DOI: 10.1128/JCM.01787-13. View

12.

Falagas M, Makris G, Dimopoulos G, Matthaiou D . Heteroresistance: a concern of increasing clinical significance?. Clin Microbiol Infect. 2007; 14(2):101-4. DOI: 10.1111/j.1469-0691.2007.01912.x. View

13.

Li J, Rayner C, Nation R, Owen R, Spelman D, Tan K . Heteroresistance to colistin in multidrug-resistant Acinetobacter baumannii. Antimicrob Agents Chemother. 2006; 50(9):2946-50. PMC: 1563544. DOI: 10.1128/AAC.00103-06. View

14.

Sanders Jr W, Sanders C . Enterobacter spp.: pathogens poised to flourish at the turn of the century. Clin Microbiol Rev. 1997; 10(2):220-41. PMC: 172917. DOI: 10.1128/CMR.10.2.220. View

15.

Meletis G, Tzampaz E, Sianou E, Tzavaras I, Sofianou D . Colistin heteroresistance in carbapenemase-producing Klebsiella pneumoniae. J Antimicrob Chemother. 2011; 66(4):946-7. DOI: 10.1093/jac/dkr007. View

16.

Ibrahim H, Thomas U, Pellegrini A . A helix-loop-helix peptide at the upper lip of the active site cleft of lysozyme confers potent antimicrobial activity with membrane permeabilization action. J Biol Chem. 2001; 276(47):43767-74. DOI: 10.1074/jbc.M106317200. View

17.

Lo-Ten-Foe J, de Smet A, Diederen B, Kluytmans J, van Keulen P . Comparative evaluation of the VITEK 2, disk diffusion, etest, broth microdilution, and agar dilution susceptibility testing methods for colistin in clinical isolates, including heteroresistant Enterobacter cloacae and Acinetobacter baumannii strains. Antimicrob Agents Chemother. 2007; 51(10):3726-30. PMC: 2043258. DOI: 10.1128/AAC.01406-06. View

18.

During K, Porsch P, Mahn A, Brinkmann O, Gieffers W . The non-enzymatic microbicidal activity of lysozymes. FEBS Lett. 1999; 449(2-3):93-100. DOI: 10.1016/s0014-5793(99)00405-6. View

19.

Fidai S, Farmer S, Hancock R . Interaction of cationic peptides with bacterial membranes. Methods Mol Biol. 1997; 78:187-204. DOI: 10.1385/0-89603-408-9:187. View

20.

Morand B, Muhlemann K . Heteroresistance to penicillin in Streptococcus pneumoniae. Proc Natl Acad Sci U S A. 2007; 104(35):14098-103. PMC: 1950099. DOI: 10.1073/pnas.0702377104. View