Estradiol Level on Day 2 and Day of Trigger: A Potential Predictor of the IVF-ET Success

Overview

Journal J Obstet Gynaecol India

Specialty Gynecology & Obstetrics

Date 2014 Jun 27

PMID 24966506

Citations 6

Authors

Sudha Prasad

Yogesh Kumar

Megha Singhal

Shashi Sharma

Affiliations

Soon will be listed here.

Abstract

Objective: To evaluate role of serum estradiol levels in predicting likelihood of pregnancy in women undergoing GnRH-a protocol in IVF-ET cycles.

Design: A 3-year retrospective analysis of estradiol levels on down-regulated day 2, day 6, and day of hCG trigger and subsequent clinical pregnancy rates.

Setting: A university hospital tertiary referral centre.

Population Or Sample: Women undergoing IVF treatment.

Methods: Hormonal assessment on the down-regulated day 2, day 6, and day of hCG trigger.

Main Outcome Measures: Comparison of hormonal profile, antral follicular count on day 2, endometrial thickness on day of trigger, and number of oocytes retrieved between pregnant and the non-pregnant group. The prediction of IVF success was based on the quantitative levels of estradiol on a specific day in down-regulated cycle.

Results: The overall pregnancy rate was 32.25 % (50/160). Estradiol level on down-regulated day 2 was 31.9 ± 12.6 and on the day of trigger was 1,996.46 ± 1,252.36 in pregnant women, which was significantly higher as compared to estradiol levels in non-pregnant women (27.6 ± 12.3 and 1,525.1 ± 1,116.42, respectively). It was found to be a significant prognostic marker for successful IVF treatment. Estradiol levels on down-regulated day 6 were found to be non-significant between the two groups.

Conclusions: Estradiol level on down-regulated day 2 of menstrual cycle and on the day of trigger was found to have a significant impact on the success of IVF-ET.

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Chen H, Cai J, Liu L, Sun X J Clin Lab Anal. 2020; 34(9):e23422.

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References

Hofmann G, Danforth D, Seifer D . Inhibin-B: the physiologic basis of the clomiphene citrate challenge test for ovarian reserve screening. Fertil Steril. 1998; 69(3):474-7. DOI: 10.1016/s0015-0282(97)00531-1. View

Weghofer A, Margreiter M, Fauster Y, Schaetz T, Brandstetter A, Boehm D . Age-specific FSH levels as a tool for appropriate patient counselling in assisted reproduction. Hum Reprod. 2005; 20(9):2448-52. DOI: 10.1093/humrep/dei076. View

Yanushpolsky E, Hurwitz S, Tikh E, Racowsky C . Predictive usefulness of cycle day 10 follicle-stimulating hormone level in a clomiphene citrate challenge test for in vitro fertilization outcome in women younger than 40 years of age. Fertil Steril. 2003; 80(1):111-5. DOI: 10.1016/s0015-0282(03)00499-0. View

Licciardi F, Liu H, Rosenwaks Z . Day 3 estradiol serum concentrations as prognosticators of ovarian stimulation response and pregnancy outcome in patients undergoing in vitro fertilization. Fertil Steril. 1995; 64(5):991-4. DOI: 10.1016/s0015-0282(16)57916-3. View

Hull M, Fleming C, Hughes A, McDermott A . The age-related decline in female fecundity: a quantitative controlled study of implanting capacity and survival of individual embryos after in vitro fertilization. Fertil Steril. 1996; 65(4):783-90. DOI: 10.1016/s0015-0282(16)58214-4. View

Tomas C, Tikkinen K, Tuomivaara L, Tapanainen J, Martikainen H . The degree of difficulty of embryo transfer is an independent factor for predicting pregnancy. Hum Reprod. 2002; 17(10):2632-5. DOI: 10.1093/humrep/17.10.2632. View

Tomas C, Nuojua-Huttunen S, Martikainen H . Pretreatment transvaginal ultrasound examination predicts ovarian responsiveness to gonadotrophins in in-vitro fertilization. Hum Reprod. 1997; 12(2):220-3. DOI: 10.1093/humrep/12.2.220. View

Scott R, Toner J, Muasher S, Oehninger S, Robinson S, Rosenwaks Z . Follicle-stimulating hormone levels on cycle day 3 are predictive of in vitro fertilization outcome. Fertil Steril. 1989; 51(4):651-4. DOI: 10.1016/s0015-0282(16)60615-5. View

Ocal P, Aydin S, Cepni I, Idil S, Idil M, Uzun H . Follicular fluid concentrations of vascular endothelial growth factor, inhibin A and inhibin B in IVF cycles: are they markers for ovarian response and pregnancy outcome?. Eur J Obstet Gynecol Reprod Biol. 2004; 115(2):194-9. DOI: 10.1016/j.ejogrb.2004.01.034. View

10.

Coroleu B, Barri P, Carreras O, Martinez F, Parriego M, Hereter L . The influence of the depth of embryo replacement into the uterine cavity on implantation rates after IVF: a controlled, ultrasound-guided study. Hum Reprod. 2002; 17(2):341-6. DOI: 10.1093/humrep/17.2.341. View

11.

Khalaf Y, Taylor A, Braude P . Low serum estradiol concentrations after five days of controlled ovarian hyperstimulation for in vitro fertilization are associated with poor outcome. Fertil Steril. 2000; 74(1):63-6. DOI: 10.1016/s0015-0282(00)00569-0. View

12.

Makker A, Singh M . Endometrial receptivity: clinical assessment in relation to fertility, infertility, and antifertility. Med Res Rev. 2006; 26(6):699-746. DOI: 10.1002/med.20061. View

13.

Hendriks D, Mol B, Bancsi L, Te Velde E, Broekmans F . Antral follicle count in the prediction of poor ovarian response and pregnancy after in vitro fertilization: a meta-analysis and comparison with basal follicle-stimulating hormone level. Fertil Steril. 2005; 83(2):291-301. DOI: 10.1016/j.fertnstert.2004.10.011. View

14.

Ficicioglu C, Kutlu T, Baglam E, Bakacak Z . Early follicular antimüllerian hormone as an indicator of ovarian reserve. Fertil Steril. 2006; 85(3):592-6. DOI: 10.1016/j.fertnstert.2005.09.019. View

15.

Scott Jr R, Hofmann G, Oehninger S, Muasher S . Intercycle variability of day 3 follicle-stimulating hormone levels and its effect on stimulation quality in in vitro fertilization. Fertil Steril. 1990; 54(2):297-302. DOI: 10.1016/s0015-0282(16)53707-8. View

16.

Edgar D . Oestrogen and human implantation. Hum Reprod. 1995; 10(1):2-4. DOI: 10.1093/humrep/10.1.2. View

17.

Papageorgiou T, Guibert J, Goffinet F, Patrat C, Fulla Y, Janssens Y . Percentile curves of serum estradiol levels during controlled ovarian stimulation in 905 cycles stimulated with recombinant FSH show that high estradiol is not detrimental to IVF outcome. Hum Reprod. 2002; 17(11):2846-50. DOI: 10.1093/humrep/17.11.2846. View

18.

Evers J . Female subfertility. Lancet. 2002; 360(9327):151-9. DOI: 10.1016/S0140-6736(02)09417-5. View

19.

Tesarik J, Mendoza C . Nongenomic effects of 17 beta-estradiol on maturing human oocytes: relationship to oocyte developmental potential. J Clin Endocrinol Metab. 1995; 80(4):1438-43. DOI: 10.1210/jcem.80.4.7714121. View

20.

Anifandis G, Koutselini E, Louridas K, Liakopoulos V, Leivaditis K, Mantzavinos T . Estradiol and leptin as conditional prognostic IVF markers. Reproduction. 2005; 129(4):531-4. DOI: 10.1530/rep.1.00567. View