CCL2 Enhances Pluripotency of Human Induced Pluripotent Stem Cells by Activating Hypoxia Related Genes

Overview

Journal Sci Rep

Specialty Science

Date 2014 Jun 25

PMID 24957798

Citations 11

Authors

Yuki Hasegawa

Dave Tang

Naoko Takahashi

Yoshihide Hayashizaki

Alistair R R Forrest

Harukazu Suzuki

Affiliations

Soon will be listed here.

Abstract

Standard culture of human induced pluripotent stem cells (hiPSCs) requires basic Fibroblast Growth Factor (bFGF) to maintain the pluripotent state, whereas hiPSC more closely resemble epiblast stem cells than true naïve state ES which requires LIF to maintain pluripotency. Here we show that chemokine (C-C motif) ligand 2 (CCL2) enhances the expression of pluripotent marker genes through the phosphorylation of the signal transducer and activator of transcription 3 (STAT3) protein. Moreover, comparison of transcriptomes between hiPSCs cultured with CCL2 versus with bFGF, we found that CCL2 activates hypoxia related genes, suggesting that CCL2 enhanced pluripotency by inducing a hypoxic-like response.Further, we show that hiPSCs cultured with CCL2 can differentiate at a higher efficiency than culturing withjust bFGF and we show CCL2 can be used in feeder-free conditions [corrected]. Taken together, our finding indicates the novel functions of CCL2 in enhancing its pluripotency in hiPSCs.

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