Synthesis of Germ-line Gamma 1 Immunoglobulin Heavy-chain Transcripts in Resting B Cells: Induction by Interleukin 4 and Inhibition by Interferon Gamma

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1989 Apr 1

PMID 2495537

Citations 48

Authors

M T Berton

J W UHR

E S Vitetta

Affiliations

Soon will be listed here.

Abstract

Interleukin 4 (IL-4) induces the expression of IgG1 and IgE in lipopolysaccharide-stimulated B cells. Previous studies have suggested that heavy-chain class switching may be regulated by increasing the accessibility of specific switch regions to switch recombinases. In this study, we have used an RNase protection assay to demonstrate that IL-4 induces expression of germ-line gamma 1 transcripts in B cells within 4 hr of culture; induction is dose-dependent and is inhibited by interferon gamma. IL-4 alone is capable of inducing the expression of germ-line gamma 1 transcripts in small, resting B cells, but lipopolysaccharide enhances expression. The germ-line transcripts are the same size (1.8 and 3.4 kilobases) as the secreted and membrane forms of the functional gamma 1 mRNAs and presumably result from the splicing of an upstream switch-region exon(s) to the gamma 1 constant-region exon(s). These data strongly support the "accessibility" model for the regulation of isotype switching and suggest that lymphokines such as IL-4 may direct specific switch events by transcriptional activation of the corresponding switch regions.

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References

Aviv H, Leder P . Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972; 69(6):1408-12. PMC: 426713. DOI: 10.1073/pnas.69.6.1408. View

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

Rogers J, Clarke P, Salser W . Sequence analysis of cloned cDNA encoding part of an immunoglobulin heavy chain. Nucleic Acids Res. 1979; 6(10):3305-21. PMC: 327935. DOI: 10.1093/nar/6.10.3305. View

Jones S, Chen Y, Isakson P, Layton J, Pure E, Word C . Effect of T cell-derived lymphokines containing B cell differentiation factor(s) for IgG (BCDF gamma) on gamma-specific mRNA in murine B cells. J Immunol. 1983; 131(6):3049-51. View

Shimizu A, Honjo T . Immunoglobulin class switching. Cell. 1984; 36(4):801-3. DOI: 10.1016/0092-8674(84)90029-1. View

Melton D, Krieg P, Rebagliati M, Maniatis T, Zinn K, Green M . Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984; 12(18):7035-56. PMC: 320141. DOI: 10.1093/nar/12.18.7035. View

NOELLE R, Krammer P, OHara J, UHR J, Vitetta E . Increased expression of Ia antigens on resting B cells: an additional role for B-cell growth factor. Proc Natl Acad Sci U S A. 1984; 81(19):6149-53. PMC: 391877. DOI: 10.1073/pnas.81.19.6149. View

Layton J, Vitetta E, UHR J, Krammer P . Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s). J Exp Med. 1984; 160(6):1850-63. PMC: 2187537. DOI: 10.1084/jem.160.6.1850. View

Yancopoulos G, Alt F . Developmentally controlled and tissue-specific expression of unrearranged VH gene segments. Cell. 1985; 40(2):271-81. DOI: 10.1016/0092-8674(85)90141-2. View

10.

Oliver K, Noelle R, UHR J, Krammer P, Vitetta E . B-cell growth factor (B-cell growth factor I or B-cell-stimulating factor, provisional 1) is a differentiation factor for resting B cells and may not induce cell growth. Proc Natl Acad Sci U S A. 1985; 82(8):2465-7. PMC: 397579. DOI: 10.1073/pnas.82.8.2465. View

11.

OHara J, Paul W . Production of a monoclonal antibody to and molecular characterization of B-cell stimulatory factor-1. Nature. 1985; 315(6017):333-6. DOI: 10.1038/315333a0. View

12.

Yuan D, Weiss E, Layton J, Krammer P, Vitetta E . Activation of the gamma 1 gene by lipopolysaccharide and T cell-derived lymphokines containing a B cell differentiation factor for IgG1 (BCDF gamma). J Immunol. 1985; 135(2):1465-9. View

13.

Vitetta E, OHara J, Myers C, Layton J, Krammer P, Paul W . Serological, biochemical, and functional identity of B cell-stimulatory factor 1 and B cell differentiation factor for IgG1. J Exp Med. 1985; 162(5):1726-31. PMC: 2187936. DOI: 10.1084/jem.162.5.1726. View

14.

Reed K, Mann D . Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res. 1985; 13(20):7207-21. PMC: 322039. DOI: 10.1093/nar/13.20.7207. View

15.

Coffman R, Carty J . A T cell activity that enhances polyclonal IgE production and its inhibition by interferon-gamma. J Immunol. 1986; 136(3):949-54. View

16.

Alt F, Blackwell T, DePinho R, Reth M, Yancopoulos G . Regulation of genome rearrangement events during lymphocyte differentiation. Immunol Rev. 1986; 89:5-30. DOI: 10.1111/j.1600-065x.1986.tb01470.x. View

17.

Radbruch A, Burger C, Klein S, Muller W . Control of immunoglobulin class switch recombination. Immunol Rev. 1986; 89:69-83. DOI: 10.1111/j.1600-065x.1986.tb01473.x. View

18.

Mowatt M, Dunnick W . DNA sequence of the murine gamma 1 switch segment reveals novel structural elements. J Immunol. 1986; 136(7):2674-83. View

19.

Sirlin S . Specificity of immunoglobulin heavy chain switch correlates with activity of germline heavy chain genes prior to switching. EMBO J. 1986; 5(1):95-102. PMC: 1166700. DOI: 10.1002/j.1460-2075.1986.tb04182.x. View

20.

Coffman R, OHara J, Bond M, Carty J, Zlotnik A, Paul W . B cell stimulatory factor-1 enhances the IgE response of lipopolysaccharide-activated B cells. J Immunol. 1986; 136(12):4538-41. View