Negative Regulation of Activation-induced Cytidine Deaminase in B Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2006 Feb 16

PMID 16477013

Citations 39

Authors

Taro Muto

Il-Mi Okazaki

Shuichi Yamada

Yoshimasa Tanaka

Kazuo Kinoshita

Masamichi Muramatsu

Hitoshi Nagaoka

Tasuku Honjo

Affiliations

Soon will be listed here.

Abstract

Both class switch recombination (CSR) and somatic hypermutation (SHM) of the Ig genes require the activity of activation-induced cytidine deaminase (AID). Expression of AID is restricted to B cells in the germinal centers of the lymphoid organs, where activated B cells undergo CSR and SHM. We previously showed that constitutive and systemic expression of AID leads to tumorigenesis in T cells and lung epithelium, but not in B cells. This finding led us to suspect that transgenic AID may be inactivated at least in part in B cells. To address this issue, we generated conditional AID-transgenic mice that constitutively express AID only in B cells. Studies on the cross between the AID-transgenic and AID-deficient mice showed that abundant AID protein accumulated by constitutive expression is inactivated in B cells, possibly providing an explanation for the absence of deregulation of CSR and SHM in AID-transgenic B cells.

Citing Articles

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