Molecular Characterization of Indian Sheeppox and Goatpox Viruses Based on RPO30 and GPCR Genes

Overview

Journal Virus Genes

Specialties Microbiology
Molecular Biology

Date 2014 Jun 23

PMID 24952423

Citations 9

Authors

Ramasamy Santhamani

Revanaiah Yogisharadhya

Gnanavel Venkatesan

Sathish Bhadravati Shivachandra

Awadh Bihari Pandey

Muthannan Andavar Ramakrishnan

Affiliations

Soon will be listed here.

Abstract

Sheeppox and goatpox are economically important diseases of small ruminants caused by sheeppox virus (SPPV) and goatpox virus (GTPV), respectively. Although SPPV and GTPV have host preference, some strains may infect both sheep and goats. As capripox viruses (SPPV, GTPV and LSDV) are antigenically related but genetically distinct, their differentiation requires analysis at molecular level. In the present study, RPO30 and GPCR genes of eight Indian SPPV and GTPV isolates were PCR amplified, cloned and sequences are genetically and phylogenetically analyzed. The RPO30 gene of SPPV and GTPV had lineage-specific signatures, and deletion of 21-nucleotide exclusively present in SPPV. Similarly, GPCR gene also had lineage-specific signatures for SPPV and GTPV. Phylogenetic analysis of capripox viruses based on RPO30 and GPCR genes revealed three distinct lineage-specific clusters as per their host origin. Our study supports that both RPO30 and GPCR genes could be used for differentiation of SPPV and GTPV as well as for molecular epidemiological studies. The study also highlights the distinct lineage specificities of the Indian SPPV and GTPV isolates including vaccine strains.

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