Nitrogen Stress Response and Stringent Response Are Coupled in Escherichia Coli

Overview

Journal Nat Commun

Specialty Biology

Date 2014 Jun 21

PMID 24947454

Citations 91

Authors

Daniel R Brown

Geraint Barton

Zhensheng Pan

Martin Buck

Sivaramesh Wigneshweraraj

Affiliations

Soon will be listed here.

Abstract

Assimilation of nitrogen is an essential process in bacteria. The nitrogen regulation stress response is an adaptive mechanism used by nitrogen-starved Escherichia coli to scavenge for alternative nitrogen sources and requires the global transcriptional regulator NtrC. In addition, nitrogen-starved E. coli cells synthesize a signal molecule, guanosine tetraphosphate (ppGpp), which serves as an effector molecule of many processes including transcription to initiate global physiological changes, collectively termed the stringent response. The regulatory mechanisms leading to elevated ppGpp levels during nutritional stresses remain elusive. Here, we show that transcription of relA, a key gene responsible for the synthesis of ppGpp, is activated by NtrC during nitrogen starvation. The results reveal that NtrC couples these two major bacterial stress responses to manage conditions of nitrogen limitation, and provide novel mechanistic insights into how a specific nutritional stress leads to elevating ppGpp levels in bacteria.

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