Activities of Three Erythrocyte Enzymes of Hyperglycemic Rats (Rattus Norvegicus) Treated with Allium Sativa Extract

Overview

Journal J Diabetes Metab Disord

Specialty Endocrinology

Date 2014 Jun 12

PMID 24918092

Authors

Paul C Chikezie

Augustine A Uwakwe

Affiliations

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Abstract

Background: The present study sought to investigate erythrocyte glutathione S-transferases (GST), NADH-Methaemoglobin reductase (NADH-MR) and Na(+)/K(+)-ATPase activities of hypoglycemic rats treated with ethanol/water (1:2 v/v) extract of A. sativa as agent of glycemic control.

Methods: Hyperglycemia was induced by a single intra-peritoneal injection of 0.1 mol/L alloxan monohydrate in phosphate buffer saline (PBS) solution (pH = 7.4); dosage = 140 mg/kg. At the end of the experimental time (t = 76 h), erythrocyte GST, NADH-MR and Na(+)/K(+)-ATPase activities as well as serum fasting blood sugar (FBS) levels were measured by spectrophotometric methods.

Results: Serum FBS levels of control/normal (C/N) rats ranged between 72.93 ± 0.82-95.12 ± 0.92 mg/dL, whereas experimental rats without glycemic control gave: 249.41 ± 1.03-256.11 ± 1.23 mg/dL. Hyperglycemic rats treated with ethanol/water (1:2 v/v) extract of A. sativa exhibited comparative reduced serum levels of FBS alongside with erythrocyte GST, NADH-MR and Na(+)/K(+)-ATPase activities. The average relative activities of the three enzymes and corresponding order of enzyme activity in hyperglycemic rats treated with ethanol/water (1:2 v/v) extract of A. sativa was: NADH-MR = 60.99% > GST = 47.81% > Na(+)/K(+)-ATPase = 46.81%. In the same order, relative activities of the three enzymes in rats without glycemic control were: NADH-MR = 49.65% > GST = 23.69% > Na(+)/K(+)-ATPase = 17.02%.

Conclusion: Erythrocyte GST, NADH-MR and Na(+)/K(+)-ATPase activities gave insights into the pathophysiology of diabetic state and served as biomarkers for ascertaining therapeutic control in Type 1 diabetes mellitus.

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