A Panel of Tumor Markers, Calreticulin, Annexin A2, and Annexin A3 in Upper Tract Urothelial Carcinoma Identified by Proteomic and Immunological Analysis

Overview

Journal BMC Cancer

Publisher Biomed Central

Specialty Oncology

Date 2014 Jun 3

PMID 24884814

Citations 14

Authors

Chih-Ming Lu

Jen-Jie Lin

Han-Hsiang Huang

Ying-Chin Ko

Jue-Liang Hsu

Jiing-Chuan Chen

Zhong-Hao Din

Yu-Jen Wu

Affiliations

Soon will be listed here.

Abstract

Background: Upper tract urothelial carcinoma (UTUC) is a tumor with sizable metastases and local recurrence. It has a worse prognosis than bladder cancer. This study was designed to investigate the urinary potential tumor markers of UTUC.

Methods: Between January 2008 and January 2009, urine was sampled from 13 patients with UTUC and 20 healthy adults. The current study identified biomarkers for UTUC using non-fixed volume stepwise weak anion exchange chromatography for fractionation of urine protein prior to two-dimensional gel electrophoresis.

Results: Fifty five differential proteins have been determined by comparing with the 2-DE maps of the urine of UTUC patients and those of healthy people. Western blotting analysis and immunohistochemistry of tumor tissues and normal tissues from patients with UTUC were carried out to further verify five possible UTUC biomarkers, including zinc-alpha-2-glycoprotein, calreticulin, annexin A2, annexin A3 and haptoglobin. The data of western blot and immunohistochemical analysis are consistent with the 2-DE data. Combined the experimental data in the urine and in tumor tissues collected from patients with UTUC, the crucial over-expressed proteins are calreticulin, annexin A2, and annexin A3.

Conclusions: Calreticulin, annexin A2, and annexin A3 are very likely a panel of biomarkers with potential value for UTUC diagnosis.

Citing Articles

Clinical Implications of the Molecular and Genomic Landscape of Upper Tract Urothelial Carcinoma.

Jaime-Casas S, Tripathi A, Pal S, Yip W Curr Urol Rep. 2024; 26(1):11.

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Prognostic analysis of anoikis-related genes in bladder cancer: An observational study.

Huang F, Zhou L, Sun J, Ma X, Pei Y, Zhang Q Medicine (Baltimore). 2024; 103(29):e38999.

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Development and validation of a model based on immunogenic cell death related genes to predict the prognosis and immune response to bladder urothelial carcinoma.

Chen L, Lin J, Wen Y, Chen Y, Chen C Front Oncol. 2023; 13:1291720.

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The intricate role of annexin A2 in kidney: a comprehensive review.

Lei J, Sun P, Sheng J, Wang H, Xie Y, Song J Ren Fail. 2023; 45(2):2273427.

PMID: 37955107 PMC: 10653649. DOI: 10.1080/0886022X.2023.2273427.

Annexin A protein family: Focusing on the occurrence, progression and treatment of cancer.

Zhang H, Zhang Z, Guo T, Chen G, Liu G, Song Q Front Cell Dev Biol. 2023; 11:1141331.

PMID: 36936694 PMC: 10020606. DOI: 10.3389/fcell.2023.1141331.

References

Yin J, Yan X, Yao X, Zhang Y, Shan Y, Mao N . Secretion of annexin A3 from ovarian cancer cells and its association with platinum resistance in ovarian cancer patients. J Cell Mol Med. 2011; 16(2):337-48. PMC: 3823297. DOI: 10.1111/j.1582-4934.2011.01316.x. View

Kikuchi E, Horiguchi Y, Nakashima J, Hatakeyama N, Matsumoto M, Nishiyama T . Lymphovascular invasion independently predicts increased disease specific survival in patients with transitional cell carcinoma of the upper urinary tract. J Urol. 2005; 174(6):2120-3. DOI: 10.1097/01.ju.0000181801.22474.8b. View

Carter K, Worwood M . Haptoglobin: a review of the major allele frequencies worldwide and their association with diseases. Int J Lab Hematol. 2007; 29(2):92-110. DOI: 10.1111/j.1751-553X.2007.00898.x. View

Marshall T, Williams K . Clinical analysis of human urinary proteins using high resolution electrophoretic methods. Electrophoresis. 1998; 19(10):1752-70. DOI: 10.1002/elps.1150191037. View

Wu N, Liu S, Guo C, Hou Z, Sun M . The role of annexin A3 playing in cancers. Clin Transl Oncol. 2012; 15(2):106-10. DOI: 10.1007/s12094-012-0928-6. View

Oldbring J, Glifberg I, MIKULOWSKI P, Hellsten S . Carcinoma of the renal pelvis and ureter following bladder carcinoma: frequency, risk factors and clinicopathological findings. J Urol. 1989; 141(6):1311-3. DOI: 10.1016/s0022-5347(17)41291-2. View

Park J, Lee D, Lee J, Park S, Kim N, Park B . Annexin A3 is a potential angiogenic mediator. Biochem Biophys Res Commun. 2005; 337(4):1283-7. DOI: 10.1016/j.bbrc.2005.10.004. View

Li W, Li C, Ke H, Wu W, Huang C, Huang C . The prognostic predictors of primary ureteral transitional cell carcinoma after radical nephroureterectomy. J Urol. 2009; 182(2):451-8. DOI: 10.1016/j.juro.2009.04.026. View

Awadallah S, Atoum M . Haptoglobin polymorphism in breast cancer patients form Jordan. Clin Chim Acta. 2004; 341(1-2):17-21. DOI: 10.1016/j.cccn.2003.10.032. View

10.

Kiernan U, Tubbs K, Nedelkov D, Niederkofler E, McConnell E, Nelson R . Comparative urine protein phenotyping using mass spectrometric immunoassay. J Proteome Res. 2003; 2(2):191-7. DOI: 10.1021/pr025574c. View

11.

Minelli A, Ronquist G, Carlsson L, Mearini E, Nilsson O, Larsson A . Antiprostasome antibody titres in benign and malignant prostate disease. Anticancer Res. 2005; 25(6C):4399-402. View

12.

Alur M, Nguyen M, Eggener S, Jiang F, Dadras S, Stern J . Suppressive roles of calreticulin in prostate cancer growth and metastasis. Am J Pathol. 2009; 175(2):882-90. PMC: 2716982. DOI: 10.2353/ajpath.2009.080417. View

13.

Carlsson M, Cederfur C, Schaar V, Balog C, Lepur A, Touret F . Galectin-1-binding glycoforms of haptoglobin with altered intracellular trafficking, and increase in metastatic breast cancer patients. PLoS One. 2011; 6(10):e26560. PMC: 3196588. DOI: 10.1371/journal.pone.0026560. View

14.

Deng R, Lu Z, Chen Y, Zhou L, Lu X . Plasma proteomic analysis of pancreatic cancer by 2-dimensional gel electrophoresis. Pancreas. 2007; 34(3):310-7. DOI: 10.1097/MPA.0b013e31802f2483. View

15.

Hisaoka M, Matsuyama A, Nakamoto M . Aberrant calreticulin expression is involved in the dedifferentiation of dedifferentiated liposarcoma. Am J Pathol. 2012; 180(5):2076-83. DOI: 10.1016/j.ajpath.2012.01.042. View

16.

Busby J, Brown G, Tamboli P, Kamat A, Dinney C, Grossman H . Upper urinary tract tumors with nontransitional histology: a single-center experience. Urology. 2006; 67(3):518-23. DOI: 10.1016/j.urology.2005.09.010. View

17.

Zhong L, Wei K, Yang X, Zhang L, Zhou X, Pan H . Increased expression of Annexin A2 in oral squamous cell carcinoma. Arch Oral Biol. 2008; 54(1):17-25. DOI: 10.1016/j.archoralbio.2008.08.006. View

18.

Skipworth R, Stewart G, Bhana M, Christie J, Sturgeon C, Guttridge D . Mass spectrometric detection of candidate protein biomarkers of cancer cachexia in human urine. Int J Oncol. 2010; 36(4):973-82. DOI: 10.3892/ijo_00000577. View

19.

Kageyama S, Isono T, Matsuda S, Ushio Y, Satomura S, Terai A . Urinary calreticulin in the diagnosis of bladder urothelial carcinoma. Int J Urol. 2009; 16(5):481-6. DOI: 10.1111/j.1442-2042.2009.02287.x. View

20.

Jamaspishvili T, Kral M, Khomeriki I, Student V, Kolar Z, Bouchal J . Urine markers in monitoring for prostate cancer. Prostate Cancer Prostatic Dis. 2009; 13(1):12-9. DOI: 10.1038/pcan.2009.31. View