Intracellular Light-activation of Riboswitch Activity

Overview

Journal Chembiochem

Specialty Biochemistry

Date 2014 May 28

PMID 24861567

Citations 11

Authors

Steven Walsh

Laura Gardner

Alexander Deiters

Gavin J Williams

Affiliations

Soon will be listed here.

Abstract

By combining a riboswitch with a cell-permeable photocaged small-molecule ligand, an optochemical gene control element was constructed that enabled spatial and temporal control of gene expression in bacterial cells. The simplicity of this strategy, coupled with the ability to create synthetic riboswitches with tailored ligand specificities and output in a variety of microorganisms, plants, and fungi might afford a general strategy to photocontrol gene expression in vivo. The ability to activate riboswitches by using light enables the interrogation and manipulation of a wide range of biological processes with high precision, and will have broad utility in the regulation of artificial genetic circuits.

Citing Articles

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Photochemical control of bacterial gene expression based on encoded genetic switches.

Paul A, Huang J, Han Y, Yang X, Vukovic L, Kral P Chem Sci. 2021; 12(7):2646-2654.

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