Regulation of Diacylglycerol Kinase δ2 Expression in C2C12 Skeletal Muscle Cells by Free Fatty Acids

Overview

Journal Lipids

Specialty Biochemistry

Date 2014 May 24

PMID 24852321

Citations 9

Authors

Shizuka Sakiyama

Takako Usuki

Hiromichi Sakai

Fumio Sakane

Affiliations

Soon will be listed here.

Abstract

Decreased expression of diacylglycerol kinase (DGK) δ in skeletal muscles is closely related to the pathogenesis of type 2 diabetes. However, the regulation of DGKδ expression is not well understood. In this study, we found that myristic acid (14:0) significantly increased DGKδ2 protein expression in a dose-dependent manner (EC(50) = 0.16 mM) in mouse C2C12 myotubes. In contrast, oleic [18:1(n-9)], eicosenoic [20:1(n-9)] and erucic [22:1(n-9)] acids markedly decreased DGKδ2 expression. Myristic acid slowly enhanced DGKδ2 expression at the transcription level. Therefore, DGKδ2 expression is positively regulated by the relatively short-chain saturated fatty acid myristic acid but attenuated by n-9 monounsaturated fatty acids.

Citing Articles

Diacylglycerol kinase δ is required for skeletal muscle development and regeneration.

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The relationship between hair metabolites, air pollution exposure and gestational diabetes mellitus: A longitudinal study from pre-conception to third trimester.

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Myristic acid selectively augments β-tubulin levels in C2C12 myotubes via diacylglycerol kinase δ.

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