Identification of Photoperiod-regulated Gene in Soybean and Functional Analysis in Nicotiana Benthamiana

Overview

Journal J Genet

Specialty Genetics

Date 2014 May 21

PMID 24840822

Citations 1

Authors

Sha Ai-Hua

Chen Yin-Hua

Shan Zhi-Hui

Zhang Xiao-Juan

Wu Xue-Jun

Qiu De-Zheng

Zhou Xin-An

Affiliations

Soon will be listed here.

Abstract

Soybean (Glycine max) is a short-day crop and the photoperiod is a crucial factor regulating its flowering time. To investigate the molecular mechanism controlling the flowering time by photoperiod in soybean, cDNA-amplified fragment length polymorphism (cDNA-AFLP) was used to identify photoperiod-regulated genes in leaves of soybean growing under short-day length, neutral photoperiod and long-day length. A total of 36 transcript-derived fragments (TDFs) were identified to be regulated by photoperiod. Among them, 26 TDFs were homologues of genes with known function. These genes are involved in secondary metabolism, cellular metabolism, cell wall components metabolism, ion transport and hormone signalling. Silencing of the homologue genes in Nicotiana benthamiana for 14 TDFs was conducted by virus-induced gene silencing. The flowering time was delayed by silencing of the genes encoding rhodanese and 40S ribosomal protein S4 (RPS4). The results indicated that rhodanese and RPS4 probably play important roles in regulating flowering time.

Citing Articles

Identification of candidate flowering and sex genes in white Guinea yam (D. rotundata Poir.) by SuperSAGE transcriptome profiling.

Girma G, Natsume S, Carluccio A, Takagi H, Matsumura H, Uemura A PLoS One. 2019; 14(9):e0216912.

PMID: 31545796 PMC: 6756524. DOI: 10.1371/journal.pone.0216912.

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