Fast Forward Genetics Based on Virus-induced Gene Silencing

Overview

Journal Curr Opin Plant Biol

Specialty Biology

Date 1999 May 14

PMID 10322199

Citations 163

Authors

D C Baulcombe

Affiliations

Soon will be listed here.

Abstract

Gene expression in plants can be suppressed in a sequence-specific manner by infection with virus vectors carrying fragments of host genes. Recent developments have revealed that the mechanism of this gene silencing is based on an RNA-mediated defence against viruses. It has also emerged that a related mechanism is involved in the post-transcriptional silencing that accounts for between line variation in transgene expression and cosuppresion of transgenes and endogenous genes. The technology of virus-induced gene silencing is being refined and adapted as a high throughput procedure for functional genomics in plants.

Citing Articles

Engineering VIGS Vectors by Modifying Movement Proteins of the 30K Family.

Villar-Alvarez D, Navarro J, Pallas V, Sanchez-Navarro J Biotechnol J. 2024; 19(12):e202400584.

PMID: 39711101 PMC: 11664229. DOI: 10.1002/biot.202400584.

Current knowledge and breeding strategies for management of aphid-transmitted viruses of pepper ( spp.) in Africa.

Zohoungbogbo H, Vihou F, Achigan-Dako E, Barchenger D Front Plant Sci. 2024; 15:1449889.

PMID: 39524558 PMC: 11543480. DOI: 10.3389/fpls.2024.1449889.

Genome-Wide Analysis of Gene Family in and Functional Exploration in Low-Temperature Response.

Yang Y, Cai Q, Luo L, Sun Z, Li L Plants (Basel). 2024; 13(4).

PMID: 38498531 PMC: 10891952. DOI: 10.3390/plants13040549.

Silencing of a Nicotiana benthamiana ascorbate oxidase gene reveals its involvement in resistance against cucumber mosaic virus.

Ahmed R, Kaldis A, Voloudakis A Planta. 2024; 259(2):38.

PMID: 38227024 PMC: 10791908. DOI: 10.1007/s00425-023-04313-x.

Ryegrass mottle virus complete genome determination and development of infectious cDNA by combining two methods- 3' RACE and RNA-Seq.

Balke I, Silamikelis I, Radovica-Spalvina I, Zeltina V, Resevica G, Fridmanis D PLoS One. 2023; 18(12):e0287278.

PMID: 38051715 PMC: 10697606. DOI: 10.1371/journal.pone.0287278.