LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2014 May 14

PMID 24818911

Citations 8

Authors

Catharine A Ortori

Nigel Halliday

Miguel Camara

Paul Williams

David A Barrett

Affiliations

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Abstract

Extracts taken from spent growth media from Pseudomonas aeruginosa can be analyzed for N-acyl-L-homoserine lactones and 2-alkyl-4-(1H)-quinolones (AQs), including the known quorum sensing signalling molecules of P. aeruginosa, in a specific and sensitive manner by liquid chromatography coupled with tandem mass spectrometric detection. This analysis can be conducted in a quantitative manner by comparison with matrix-matched calibration samples.

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Whole-Cell Biosensors for Qualitative and Quantitative Analysis of Quorum Sensing Signal Molecules and the Investigation of Quorum Quenching Agents.

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