Differential Expression of Stress and Immune Response Pathway Transcripts and MiRNAs in Normal Human Endothelial Cells Subjected to Fractionated or Single-dose Radiation

Overview

Journal Mol Cancer Res

Specialty Cell Biology

Date 2014 May 3

PMID 24784841

Citations 28

Authors

Sanjeewani T Palayoor

Molykutty John-Aryankalayil

Adeola Y Makinde

Michael T Falduto

Scott R Magnuson

C Norman Coleman

Affiliations

Soon will be listed here.

Abstract

Unlabelled: Although modern radiotherapy technologies can precisely deliver higher doses of radiation to tumors, thus, reducing overall radiation exposure to normal tissues, moderate dose, and normal tissue toxicity still remains a significant limitation. The present study profiled the global effects on transcript and miR expression in human coronary artery endothelial cells using single-dose irradiation (SD, 10 Gy) or multifractionated irradiation (MF, 2 Gy × 5) regimens. Longitudinal time points were collected after an SD or final dose of MF irradiation for analysis using Agilent Human Gene Expression and miRNA microarray platforms. Compared with SD, the exposure to MF resulted in robust transcript and miR expression changes in terms of the number and magnitude. For data analysis, statistically significant mRNAs (2-fold) and miRs (1.5-fold) were processed by Ingenuity Pathway Analysis to uncover miRs associated with target transcripts from several cellular pathways after irradiation. Interestingly, MF radiation induced a cohort of mRNAs and miRs that coordinate the induction of immune response pathway under tight regulation. In addition, mRNAs and miRs associated with DNA replication, recombination and repair, apoptosis, cardiovascular events, and angiogenesis were revealed.

Implications: Radiation-induced alterations in stress and immune response genes in endothelial cells contribute to changes in normal tissue and tumor microenvironment, and affect the outcome of radiotherapy.

Citing Articles

Comparative Analysis of miRNA Expression after Whole-Body Irradiation Across Three Strains of Mice.

Martello S, Bylicky M, Shankavaram U, May J, Chopra S, Sproull M Radiat Res. 2023; 200(3):266-280.

PMID: 37527359 PMC: 10635637. DOI: 10.1667/RADE-23-00007.1.

Emerging evidence for adapting radiotherapy to immunotherapy.

Galluzzi L, Aryankalayil M, Coleman C, Formenti S Nat Rev Clin Oncol. 2023; 20(8):543-557.

PMID: 37280366 DOI: 10.1038/s41571-023-00782-x.

Profiling mRNA, miRNA and lncRNA expression changes in endothelial cells in response to increasing doses of ionizing radiation.

Chopra S, Shankavaram U, Bylicky M, Dalo J, Scott K, Aryankalayil M Sci Rep. 2022; 12(1):19941.

PMID: 36402833 PMC: 9675751. DOI: 10.1038/s41598-022-24051-6.

Long-term expression changes of immune-related genes in prostate cancer after radiotherapy.

Eke I, Aryankalayil M, Bylicky M, Sandfort V, Vanpouille-Box C, Nandagopal S Cancer Immunol Immunother. 2021; 71(4):839-850.

PMID: 34435232 PMC: 8873240. DOI: 10.1007/s00262-021-03036-w.

Analysis of lncRNA-miRNA-mRNA expression pattern in heart tissue after total body radiation in a mouse model.

Aryankalayil M, Martello S, Bylicky M, Chopra S, May J, Shankardass A J Transl Med. 2021; 19(1):336.

PMID: 34364390 PMC: 8349067. DOI: 10.1186/s12967-021-02998-w.

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