Comparative Anti-microbial Efficacy of Azadirachta Indica Irrigant with Standard Endodontic Irrigants: A Preliminary Study

Overview

Journal J Conserv Dent

Specialty Dentistry

Date 2014 Apr 30

PMID 24778508

Citations 13

Authors

Arindam Dutta

Mala Kundabala

Affiliations

Soon will be listed here.

Abstract

Objective: The anti-microbial efficacy of 2.5% sodium hypochlorite (SHC) and 0.2% chlorhexidine gluconate were compared with an experimental irrigant formulated from the Neem tree, Azadirachta indica A. Juss.

Materials And Methods: A sample of 36 single rooted anterior teeth with periapical radiolucency and absence of response to vitality tests that required root canal treatment were selected for this study. The test irrigants and their combinations were assigned to five different groups and saline served as the control. Access cavities were prepared using an aseptic technique and samples collected for both anaerobic culture and Gram stained smears, followed by irrigation and sample collection again. The number of organisms were expressed in colony forming units/ml after 72 h of incubation; the smears were analyzed for their microbial loads and tissue clearance and assessed as per defined criteria.

Results: Our results found the maximum reduction in microbial loads, when analyzed by culture method, with a combination of SHC and the experimental neem irrigant. Maximum tissue clearance on the Gram Stained smears was also found with the same combination.

Conclusion: Neem irrigant has anti-microbial efficacy and can be considered for endodontic use.

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References

Jain A, Basal E . Inhibition of Propionibacterium acnes-induced mediators of inflammation by Indian herbs. Phytomedicine. 2003; 10(1):34-8. DOI: 10.1078/094471103321648638. View

Kuruvilla J, Kamath M . Antimicrobial activity of 2.5% sodium hypochlorite and 0.2% chlorhexidine gluconate separately and combined, as endodontic irrigants. J Endod. 1998; 24(7):472-6. DOI: 10.1016/S0099-2399(98)80049-6. View

Cunningham W, Cole 3rd J, BALEKJIAN A . Effect of alcohol on the spreading ability of sodium hypochlorite endodontic irrigant. Oral Surg Oral Med Oral Pathol. 1982; 54(3):333-5. DOI: 10.1016/0030-4220(82)90105-0. View

Tanomaru Filho M, Leonardo M, Silva L, Anibal F, Faccioli L . Inflammatory response to different endodontic irrigating solutions. Int Endod J. 2002; 35(9):735-9. DOI: 10.1046/j.1365-2591.2002.00544.x. View

Naenni N, Thoma K, Zehnder M . Soft tissue dissolution capacity of currently used and potential endodontic irrigants. J Endod. 2004; 30(11):785-7. DOI: 10.1097/00004770-200411000-00009. View

Siqueira Jr J, Batista M, Fraga R, de Uzeda M . Antibacterial effects of endodontic irrigants on black-pigmented gram-negative anaerobes and facultative bacteria. J Endod. 1998; 24(6):414-6. DOI: 10.1016/S0099-2399(98)80023-X. View

Fabry W, Okemo P, Ansorg R . Antibacterial activity of East African medicinal plants. J Ethnopharmacol. 1998; 60(1):79-84. DOI: 10.1016/s0378-8741(97)00128-1. View

Rosaline H, Kandaswamy D, Gogulnath D, Rubin M . Influence of various herbal irrigants as a final rinse on the adherence of Enterococcus faecalis by fluorescence confocal laser scanning microscope. J Conserv Dent. 2013; 16(4):352-5. PMC: 3740649. DOI: 10.4103/0972-0707.114365. View

Okpanyi S, Ezeukwu G . Anti-inflammatory and antipyretic activities of Azadirachta indica. Planta Med. 1981; 41(1):34-9. DOI: 10.1055/s-2007-971670. View

10.

Fabry W, Okemo P, Ansorg R . Fungistatic and fungicidal activity of east African medicinal plants. Mycoses. 1996; 39(1-2):67-70. DOI: 10.1111/j.1439-0507.1996.tb00087.x. View

11.

Luddin N, Ahmed H . The antibacterial activity of sodium hypochlorite and chlorhexidine against Enterococcus faecalis: A review on agar diffusion and direct contact methods. J Conserv Dent. 2013; 16(1):9-16. PMC: 3548356. DOI: 10.4103/0972-0707.105291. View

12.

Dutta A, Kundabala M . Antimicrobial efficacy of endodontic irrigants from Azadirachta indica: An in vitro study. Acta Odontol Scand. 2013; 71(6):1594-8. DOI: 10.3109/00016357.2013.780290. View

13.

Chen C, Wu R . Gallotannins inhibit growth, water-insoluble glucan synthesis, and aggregation of mutans streptococci. J Dent Res. 1988; 67(1):51-5. DOI: 10.1177/00220345880670011001. View

14.

Wolinsky L, Mania S, Nachnani S, Ling S . The inhibiting effect of aqueous Azadirachta indica (Neem) extract upon bacterial properties influencing in vitro plaque formation. J Dent Res. 1996; 75(2):816-22. DOI: 10.1177/00220345960750021301. View

15.

Pai M, Acharya L, Udupa N . Evaluation of antiplaque activity of Azadirachta indica leaf extract gel--a 6-week clinical study. J Ethnopharmacol. 2003; 90(1):99-103. DOI: 10.1016/j.jep.2003.09.035. View

16.

Vanka A, Tandon S, Rao S, Udupa N, Ramkumar P . The effect of indigenous Neem Azadirachta indica [correction of (Adirachta indica)] mouth wash on Streptococcus mutans and lactobacilli growth. Indian J Dent Res. 2002; 12(3):133-44. View

17.

van der Nat J, van der Sluis W, de Silva K, Labadie R . Ethnopharmacognostical survey of Azadirachta indica A. Juss (Meliaceae). J Ethnopharmacol. 1991; 35(1):1-24. DOI: 10.1016/0378-8741(91)90131-v. View

18.

Ballal N, Kundabala M, Bhat K, Acharya S, Ballal M, Kumar R . Susceptibility of Candida albicans and Enterococcus faecalis to Chitosan, Chlorhexidine gluconate and their combination in vitro. Aust Endod J. 2009; 35(1):29-33. DOI: 10.1111/j.1747-4477.2008.00126.x. View

19.

Nwosu M, Okafor J . Preliminary studies of the antifungal activities of some medicinal plants against Basidiobolus and some other pathogenic fungi. Mycoses. 1995; 38(5-6):191-5. DOI: 10.1111/j.1439-0507.1995.tb00048.x. View

20.

Moller A . Microbiological examination of root canals and periapical tissues of human teeth. Methodological studies. Odontol Tidskr. 1966; 74(5):Suppl:1-380. View