Age-dependent Changes of Nucleic Acid Labeling in Different Rat Brain Regions

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1989 Jul 1

PMID 2476678

Citations 2

Authors

D F Condorelli

R Avola

N Ragusa

S Reale

M Renis

R F Villa

A M Giuffrida Stella

Affiliations

Soon will be listed here.

Abstract

The effects of aging on in vivo DNA and RNA labeling and on RNA content in various brain regions of 4-, 12-, and 24-month-old rats were investigated. No difference in [methyl-14C]thymidine incorporation into DNA of cerebral cortex and cerebellum during aging was observed. The ratio of RNA/DNA content significantly decreased from 4 to 24 months of age in cerebral cortex, cerebellum and striatum. RNA labeling decreased by 15% in cerebral cortex of 24-month-old animals while in the other brain areas examined (cerebellum, hippocampus, hypothalamus, brainstem, striatum) did not change during aging. In the cerebral cortex, the ratio of the specific radioactivity of microsomal RNA to that of nuclear RNA, determined by in vivo experiments, was not affected by the aging process. A significant decrease of total, poly(A) RNA and poly(A) RNA content was observed in the same brain area of 24-month-old rats compared to 4-month-old ones. Moreover, densitometric and radioactivity patterns obtained by gel electrophoresis of labeled RNA after in vitro experiments (tissue slices of cerebral cortex) showed a different ribosomal RNA processing during aging. In vivo chronic treatment with CDP-choline was able to increase RNA labeling in corpus striatum of 24-month-old animals.

Citing Articles

Nakane H, Nakahara T, Yao H, Ooboshi H, Ibayashi S, Uchimura H Neurochem Res. 1997; 22(3):253-6.

PMID: 9051658 DOI: 10.1023/a:1022478419450.

Changes of mitochondrial cytochrome c oxidase and FoF1 ATP synthase subunits in rat cerebral cortex during aging.

Nicoletti V, Tendi E, Lalicata C, Reale S, Costa A, Villa R Neurochem Res. 1995; 20(12):1465-70.

PMID: 8789609 DOI: 10.1007/BF00970595.

References

Wannemacher Jr R, Banks Jr W, Wunner W . Use of a single tissue extract to determine cellular protein and nucleic acid concentrations and rate of amino acid incorporation. Anal Biochem. 1965; 11(2):320-6. DOI: 10.1016/0003-2697(65)90020-5. View

Colman P, Kaplan B, Osterburg H, Finch C . Brain poly(A)RNA during aging: stability of yield and sequence complexity in two rat strains. J Neurochem. 1980; 34(2):335-45. DOI: 10.1111/j.1471-4159.1980.tb06602.x. View

Giufrida A, Cox D, Mathias A . RNA polymerade activity in various classes of nuclei from different regions of rat brain during postnatal development. J Neurochem. 1975; 24(4):749-55. View

Fando J, Salinas M, Wasterlain C . Age-dependent changes in brain protein synthesis in the rat. Neurochem Res. 1980; 5(4):373-83. DOI: 10.1007/BF00964226. View

Aviv H, Leder P . Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972; 69(6):1408-12. PMC: 426713. DOI: 10.1073/pnas.69.6.1408. View

Schroder H, Messer R, Bachmann M, Bernd A, Muller W . Superoxide radical-induced loss of nuclear restriction of immature mRNA: a possible cause for ageing. Mech Ageing Dev. 1987; 41(3):251-66. DOI: 10.1016/0047-6374(87)90045-5. View

Dwyer B, Fando J, Wasterlain C . Rat brain protein synthesis declines during postdevelopmental aging. J Neurochem. 1980; 35(3):746-9. DOI: 10.1111/j.1471-4159.1980.tb03717.x. View

Ringborg U . Composition and content of RNA in neurons of rat hippocampus at different ages. Brain Res. 1966; 2(3):296-8. DOI: 10.1016/0006-8993(66)90051-5. View

Berkowitz E, Sanborn A, Vaughan D . Chromatin structure in neuronal and neuroglial cell nuclei as a function of age. J Neurochem. 1983; 41(2):516-23. DOI: 10.1111/j.1471-4159.1983.tb04769.x. View

10.

Cosgrove J, Rapoport S . Absence of age differences in protein synthesis by rat brain, measured with an initiating cell-free system. Neurobiol Aging. 1987; 8(1):27-34. DOI: 10.1016/0197-4580(87)90054-6. View

11.

Makrides S . Protein synthesis and degradation during aging and senescence. Biol Rev Camb Philos Soc. 1983; 58(3):343-422. DOI: 10.1111/j.1469-185x.1983.tb00394.x. View

12.

Serra I, Cupello A, Gadaleta M, Viola M, Ragonese P, GIUFFRIDA A . Labeling of RNA in young and adult rat brain: evidence for different RNA processing. Neurochem Res. 1983; 8(4):433-47. DOI: 10.1007/BF00965100. View

13.

MENZIES R, GOLD P . The apparent turnover of mitochondria, ribosomes and sRNA of the brain in young adult and aged rats. J Neurochem. 1972; 19(7):1671-83. DOI: 10.1111/j.1471-4159.1972.tb06212.x. View

14.

Lai J, Walsh J, Dennis S, Clark J . Synaptic and non-synaptic mitochondria from rat brain: isolation and characterization. J Neurochem. 1977; 28(3):625-31. DOI: 10.1111/j.1471-4159.1977.tb10434.x. View

15.

Ekstrom R, Liu D, Richardson A . Changes in brain protein synthesis during the life span of male Fischer rats. Gerontology. 1980; 26(3):121-8. DOI: 10.1159/000212405. View

16.

Rigoulet M, Guerin B, Cohadon F, Vandendreissche M . Unilateral brain injury in the rabbit: reversible and irreversible damage of the membranal ATPases. J Neurochem. 1979; 32(2):535-41. DOI: 10.1111/j.1471-4159.1979.tb00381.x. View

17.

Benzi G, Arrigoni E, Pastoris O, Villa R, Dossena M, Agnoli A . Drug action on the metabolic changes induced by acute hypoxia on synaptosomes from the cerebral cortex. J Cereb Blood Flow Metab. 1982; 2(2):229-39. DOI: 10.1038/jcbfm.1982.23. View

18.

Serra I, Alberghina M, Viola M, Mistretta A, GIUFFRIDA A . Effect of CDP-choline on the biosynthesis of nucleic acids and proteins in brain regions during hypoxia. Neurochem Res. 1981; 6(6):607-18. DOI: 10.1007/BF00963878. View

19.

Chaconas G, Finch C . The effect of ageing on RNA-DNA ratios in brain regions of the C57BL-6J male mouse. J Neurochem. 1973; 21(6):1469-73. DOI: 10.1111/j.1471-4159.1973.tb06030.x. View

20.

Ingvar M, Maeder P, Sokoloff L, Smith C . Effects of ageing on local rates of cerebral protein synthesis in Sprague-Dawley rats. Brain. 1985; 108 ( Pt 1):155-70. DOI: 10.1093/brain/108.1.155. View