Astrocytes and Microglia in Human Brain Share an Epitope Recognized by a B-lymphocyte-specific Monoclonal Antibody (LN-1)

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 1989 Jul 1

PMID 2476034

Citations 9

Authors

D W Dickson

L A Mattiace

Affiliations

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Abstract

A B-lymphocyte-specific mouse monoclonal antibody, LN-1, recognizes two morphologic classes of glial cells in human brain. The nature and duration of tissue fixation and processing are critical in the detection of the two cell types. In tissue that is lightly fixed, LN-1 recognizes astrocytes. The astrocytic nature of the LN-1 reactive glial cell was confirmed by cytologic features, tissue distribution, immunoelectron microscopy, double labeling immunofluorescent microscopy, and staining of serial sections with antibodies to glial fibrillary acidic protein. In tissue that is fixed for longer periods or in Bouin's fixative, two glial cell types are recognized: astrocytes and microglia. The identity of the latter cell type as microglia was confirmed by morphologic features, tissue distribution, immunoelectron microscopy, and double staining with monoclonal antibodies or lectins to macrophage markers, including class II major histocompatibility antigens. The two cell types had different disposition in senile plaques of elderly individuals and of those with Alzheimer's disease. Astrocytes were present at the periphery of the plaques, whereas microglial cells were centrally placed, often in juxtaposition to amyloid. The results are discussed with respect to ontogeny of glial cells and the ability of monoclonal antibodies to recognize epitopes on unrelated proteins.

Citing Articles

Discovery of new lesions in neurodegenerative diseases with monoclonal antibody techniques: is there a non-amyloid precursor to senile plaques?.

Dickson D Am J Pathol. 1997; 151(1):7-11.

PMID: 9212725 PMC: 1857943.

Molecular profile of reactive astrocytes--implications for their role in neurologic disease.

Eddleston M, Mucke L Neuroscience. 1993; 54(1):15-36.

PMID: 8515840 PMC: 7130906. DOI: 10.1016/0306-4522(93)90380-x.

Immunohistochemical study of microglia in the Creutzfeldt-Jakob diseased brain.

Sasaki A, Hirato J, Nakazato Y Acta Neuropathol. 1993; 86(4):337-44.

PMID: 8256583 DOI: 10.1007/BF00369445.

Microglia in cerebellar plaques in Alzheimer's disease.

Mattiace L, Davies P, Yen S, Dickson D Acta Neuropathol. 1990; 80(5):493-8.

PMID: 2251906 DOI: 10.1007/BF00294609.

Ubiquitin immunoelectron microscopy of dystrophic neurites in cerebellar senile plaques of Alzheimer's disease.

Dickson D, Wertkin A, Mattiace L, Fier E, Kress Y, Davies P Acta Neuropathol. 1990; 79(5):486-93.

PMID: 2158201 DOI: 10.1007/BF00296107.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Perentes E, Rubinstein L . Non-specific binding of mouse myeloma IgM immunoglobulins by human myelin sheaths and astrocytes. A potential complication of nervous system immunoperoxidase histochemistry. Acta Neuropathol. 1986; 70(3-4):284-8. DOI: 10.1007/BF00686085. View

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

Lane D, KOPROWSKI H . Molecular recognition and the future of monoclonal antibodies. Nature. 1982; 296(5854):200-2. DOI: 10.1038/296200a0. View

BRIERLEY J, Brown A . The origin of lipid phagocytes in the central nervous system: I. The intrinsic microglia. J Comp Neurol. 1982; 211(4):397-406. DOI: 10.1002/cne.902110406. View

Epstein A, Marder R, Winter J, Fox R . Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors. J Immunol. 1984; 133(2):1028-36. View

Perry V, Hume D, Gordon S . Immunohistochemical localization of macrophages and microglia in the adult and developing mouse brain. Neuroscience. 1985; 15(2):313-26. DOI: 10.1016/0306-4522(85)90215-5. View

Yen S, Crowe A, Dickson D . Monoclonal antibodies to Alzheimer neurofibrillary tangles. 1. Identification of polypeptides. Am J Pathol. 1985; 120(2):282-91. PMC: 1887824. View

Wolozin B, Pruchnicki A, Dickson D, Davies P . A neuronal antigen in the brains of Alzheimer patients. Science. 1986; 232(4750):648-50. DOI: 10.1126/science.3083509. View

10.

Dickson D . Multinucleated giant cells in acquired immunodeficiency syndrome encephalopathy. Origin from endogenous microglia?. Arch Pathol Lab Med. 1986; 110(10):967-8. View

11.

Mannoji H, Yeger H, Becker L . A specific histochemical marker (lectin Ricinus communis agglutinin-1) for normal human microglia, and application to routine histopathology. Acta Neuropathol. 1986; 71(3-4):341-3. DOI: 10.1007/BF00688060. View

12.

Sato Y, Mukai K, Watanabe S, Goto M, Shimosato Y . The AMeX method. A simplified technique of tissue processing and paraffin embedding with improved preservation of antigens for immunostaining. Am J Pathol. 1986; 125(3):431-5. PMC: 1888473. View

13.

Hansen L, Armstrong D, Terry R . An immunohistochemical quantification of fibrous astrocytes in the aging human cerebral cortex. Neurobiol Aging. 1987; 8(1):1-6. DOI: 10.1016/0197-4580(87)90051-0. View

14.

Ksiezak-Reding H, Dickson D, Davies P, Yen S . Recognition of tau epitopes by anti-neurofilament antibodies that bind to Alzheimer neurofibrillary tangles. Proc Natl Acad Sci U S A. 1987; 84(10):3410-4. PMC: 304880. DOI: 10.1073/pnas.84.10.3410. View

15.

Hayes G, Woodroofe M, Cuzner M . Microglia are the major cell type expressing MHC class II in human white matter. J Neurol Sci. 1987; 80(1):25-37. DOI: 10.1016/0022-510x(87)90218-8. View

16.

Dickson D, Ksiezak-Reding H, Davies P, Yen S . A monoclonal antibody that recognizes a phosphorylated epitope in Alzheimer neurofibrillary tangles, neurofilaments and tau proteins immunostains granulovacuolar degeneration. Acta Neuropathol. 1987; 73(3):254-8. DOI: 10.1007/BF00686619. View

17.

Gebicke-Haerter P, Bauer J, Brenner A, Gerok W . Alpha 2-macroglobulin synthesis in an astrocyte subpopulation. J Neurochem. 1987; 49(4):1139-45. DOI: 10.1111/j.1471-4159.1987.tb10004.x. View

18.

Streit W, Kreutzberg G . Lectin binding by resting and reactive microglia. J Neurocytol. 1987; 16(2):249-60. DOI: 10.1007/BF01795308. View

19.

Hickey W, Kimura H . Perivascular microglial cells of the CNS are bone marrow-derived and present antigen in vivo. Science. 1988; 239(4837):290-2. DOI: 10.1126/science.3276004. View

20.

JORDAN F, THOMAS W . Brain macrophages: questions of origin and interrelationship. Brain Res. 1988; 472(2):165-78. DOI: 10.1016/0165-0173(88)90019-7. View