PhiC31 Integrase Induces Efficient Site-specific Recombination in the Capra Hircus Genome

Overview

Journal DNA Cell Biol

Publisher Mary Ann Liebert

Specialties Cell Biology
Molecular Biology

Date 2014 Apr 24

PMID 24754538

Citations 4

Authors

Haiyan Ma

Qingwen Ma

Yao Lu

Juan Wang

Wei Hu

Zhijuan Gong

Linlin Cai

Ying Huang

Shu-Zhen Huang

Fanyi Zeng

Affiliations

Soon will be listed here.

Abstract

Streptomyces phage φC31 integrase induces efficient site-specific recombination capable of integrating exogenous genes at pseudo attP sites in human, mouse, rat, rabbit, sheep, Drosophila, and bovine genomes. However, the φC31-mediated recombination between attB and the corresponding pseudo attP sites has not been investigated in Capra hircus. Here, we identified eight pseudo attP sites located in the intron or intergenic regions of the C. hircus genome, and demonstrated different levels of foreign gene expression after φC31 integrase-mediated integration. These pseudo attP sites share similar sequences with each other and with pseudo attP sites in other mammalian genomes, and these are associated with a neighboring consensus motif found in other genomes. The application of the φC31 integrase system in C. hircus provides a new option for genetic engineering of this economically important goat species.

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