IFN-dependent and -independent Reduction in West Nile Virus Infectivity in Human Dermal Fibroblasts

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2014 Mar 26

PMID 24662674

Citations 5

Authors

Lisa I Hoover

Brenda L Fredericksen

Affiliations

Soon will be listed here.

Abstract

Although dermal fibroblasts are one of the first cell types exposed to West Nile virus (WNV) during a blood meal by an infected mosquito, little is known about WNV replication within this cell type. Here, we demonstrate that neuroinvasive, WNV-New York (WNV-NY), and nonneuroinvasive, WNV-Australia (WNV-AUS60) strains are able to infect and replicate in primary human dermal fibroblasts (HDFs). However, WNV-AUS60 replication and spread within HDFs was reduced compared to that of WNV-NY due to an interferon (IFN)-independent reduction in viral infectivity early in infection. Additionally, replication of both strains was constrained late in infection by an IFN-β-dependent reduction in particle infectivity. Overall, our data indicates that human dermal fibroblasts are capable of supporting WNV replication; however, the low infectivity of particles produced from HDFs late in infection suggests that this cell type likely plays a limited role as a viral reservoir in vivo.

Citing Articles

CD11b maintains West Nile virus replication through modulation of immune response in human neuroblastoma cells.

Liu Y, Peng H, Ren R, Zhao P, Zhao L Virol J. 2024; 21(1):158.

PMID: 39004752 PMC: 11247799. DOI: 10.1186/s12985-024-02427-6.

Limostatin and Its Human Ortholog Promote West Nile Virus Infection.

Mead E, Lee M, Trammell C, Goodman A Insects. 2024; 15(6).

PMID: 38921161 PMC: 11203814. DOI: 10.3390/insects15060446.

Pathogenicity and virulence of West Nile virus revisited eight decades after its first isolation.

Saiz J, Martin-Acebes M, Blazquez A, Escribano-Romero E, Poderoso T, Jimenez de Oya N Virulence. 2021; 12(1):1145-1173.

PMID: 33843445 PMC: 8043182. DOI: 10.1080/21505594.2021.1908740.

Host sphingomyelin increases West Nile virus infection in vivo.

Martin-Acebes M, Gabande-Rodriguez E, Garcia-Cabrero A, Sanchez M, Ledesma M, Sobrino F J Lipid Res. 2016; 57(3):422-32.

PMID: 26764042 PMC: 4766991. DOI: 10.1194/jlr.M064212.

Soluble mediators produced by the crosstalk between microvascular endothelial cells and dengue-infected primary dermal fibroblasts inhibit dengue virus replication and increase leukocyte transmigration.

Bustos-Arriaga J, Mita-Mendoza N, Lopez-Gonzalez M, Garcia-Cordero J, Juarez-Delgado F, Gromowski G Immunol Res. 2015; 64(2):392-403.

PMID: 26130295 DOI: 10.1007/s12026-015-8675-8.

References

Styer L, Lim P, Louie K, Albright R, Kramer L, Bernard K . Mosquito saliva causes enhancement of West Nile virus infection in mice. J Virol. 2010; 85(4):1517-27. PMC: 3028906. DOI: 10.1128/JVI.01112-10. View

Heinz F, Stiasny K, Holzmann H, Allison S, Mandl C, Kunz C . Structural changes and functional control of the tick-borne encephalitis virus glycoprotein E by the heterodimeric association with protein prM. Virology. 1994; 198(1):109-17. DOI: 10.1006/viro.1994.1013. View

Shi P, Tilgner M, Lo M, Kent K, Bernard K . Infectious cDNA clone of the epidemic west nile virus from New York City. J Virol. 2002; 76(12):5847-56. PMC: 136194. DOI: 10.1128/jvi.76.12.5847-5856.2002. View

Arjona A, Ledizet M, Anthony K, Bonafe N, Modis Y, Town T . West Nile virus envelope protein inhibits dsRNA-induced innate immune responses. J Immunol. 2007; 179(12):8403-9. DOI: 10.4049/jimmunol.179.12.8403. View

Wong P, YUEN P, MacLeod R, Chang E, Myers M, Friedman R . The effect of interferon on de novo infection of Moloney murine leukemia virus. Cell. 1977; 10(2):245-52. DOI: 10.1016/0092-8674(77)90218-5. View

Magurano F, Remoli M, Baggieri M, Fortuna C, Marchi A, Fiorentini C . Circulation of West Nile virus lineage 1 and 2 during an outbreak in Italy. Clin Microbiol Infect. 2012; 18(12):E545-7. DOI: 10.1111/1469-0691.12018. View

Best S, Morris K, Shannon J, Robertson S, Mitzel D, Park G . Inhibition of interferon-stimulated JAK-STAT signaling by a tick-borne flavivirus and identification of NS5 as an interferon antagonist. J Virol. 2005; 79(20):12828-39. PMC: 1235813. DOI: 10.1128/JVI.79.20.12828-12839.2005. View

Byrne S, Halliday G, Johnston L, King N . Interleukin-1beta but not tumor necrosis factor is involved in West Nile virus-induced Langerhans cell migration from the skin in C57BL/6 mice. J Invest Dermatol. 2001; 117(3):702-9. DOI: 10.1046/j.0022-202x.2001.01454.x. View

Reagan K, Machain-Williams C, Wang T, Blair C . Immunization of mice with recombinant mosquito salivary protein D7 enhances mortality from subsequent West Nile virus infection via mosquito bite. PLoS Negl Trop Dis. 2012; 6(12):e1935. PMC: 3516580. DOI: 10.1371/journal.pntd.0001935. View

10.

Venter M, Myers T, Wilson M, Kindt T, Paweska J, Burt F . Gene expression in mice infected with West Nile virus strains of different neurovirulence. Virology. 2005; 342(1):119-40. DOI: 10.1016/j.virol.2005.07.013. View

11.

Colpitts T, Conway M, Montgomery R, Fikrig E . West Nile Virus: biology, transmission, and human infection. Clin Microbiol Rev. 2012; 25(4):635-48. PMC: 3485754. DOI: 10.1128/CMR.00045-12. View

12.

Palmer D, Sun P, Celluzzi C, Bisbing J, Pang S, Sun W . Differential effects of dengue virus on infected and bystander dendritic cells. J Virol. 2005; 79(4):2432-9. PMC: 546567. DOI: 10.1128/JVI.79.4.2432-2439.2005. View

13.

Bakonyi T, Ferenczi E, Erdelyi K, Kutasi O, Csorgo T, Seidel B . Explosive spread of a neuroinvasive lineage 2 West Nile virus in Central Europe, 2008/2009. Vet Microbiol. 2013; 165(1-2):61-70. DOI: 10.1016/j.vetmic.2013.03.005. View

14.

Styer L, Kent K, Albright R, Bennett C, Kramer L, Bernard K . Mosquitoes inoculate high doses of West Nile virus as they probe and feed on live hosts. PLoS Pathog. 2007; 3(9):1262-70. PMC: 1976553. DOI: 10.1371/journal.ppat.0030132. View

15.

Botha E, Markotter W, Wolfaardt M, Paweska J, Swanepoel R, Palacios G . Genetic determinants of virulence in pathogenic lineage 2 West Nile virus strains. Emerg Infect Dis. 2008; 14(2):222-30. PMC: 2600181. DOI: 10.3201/eid1402.070457. View

16.

Wilson J, de Sessions P, Leon M, Scholle F . West Nile virus nonstructural protein 1 inhibits TLR3 signal transduction. J Virol. 2008; 82(17):8262-71. PMC: 2519649. DOI: 10.1128/JVI.00226-08. View

17.

Liu W, Chen H, Wang X, Huang H, Khromykh A . Analysis of adaptive mutations in Kunjin virus replicon RNA reveals a novel role for the flavivirus nonstructural protein NS2A in inhibition of beta interferon promoter-driven transcription. J Virol. 2004; 78(22):12225-35. PMC: 525072. DOI: 10.1128/JVI.78.22.12225-12235.2004. View

18.

Fredericksen B, Smith M, Katze M, Shi P, Gale Jr M . The host response to West Nile Virus infection limits viral spread through the activation of the interferon regulatory factor 3 pathway. J Virol. 2004; 78(14):7737-47. PMC: 434084. DOI: 10.1128/JVI.78.14.7737-7747.2004. View

19.

Scherbik S, Stockman B, Brinton M . Differential expression of interferon (IFN) regulatory factors and IFN-stimulated genes at early times after West Nile virus infection of mouse embryo fibroblasts. J Virol. 2007; 81(21):12005-18. PMC: 2168811. DOI: 10.1128/JVI.01359-07. View

20.

Danis K, Papa A, Theocharopoulos G, Dougas G, Athanasiou M, Detsis M . Outbreak of West Nile virus infection in Greece, 2010. Emerg Infect Dis. 2011; 17(10):1868-72. PMC: 3310677. DOI: 10.3201/eid1710.110525. View