Identification of Simian Immunodeficiency Virus SIVMAC Env Gene Products

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1989 Mar 1

PMID 2464704

Citations 13

Authors

F D Veronese

B Joseph

T D Copeland

S Oroszlan

R C Gallo

M G Sarngadharan

Affiliations

Soon will be listed here.

Abstract

A monoclonal antibody recognizing an antigenic determinant on the env transmembrane protein, gp32 of simian immunodeficiency virus SIVMAC has been developed and designated SF8/5E11. The reactivity of this antibody was found to be type specific, since it did not cross-react with either SIVSMM or SIVMNe transmembrane proteins. The availability of both this antibody and the complete nucleotide sequence of SIVMAC allowed us to define the organization of the env gene products of this virus. Radiolabel sequencing of the amino termini of both gp160 and gp32 confirmed the positions of both cleavage sites predicted by alignment of the inferred amino acid sequences of the SIVMAC and human immunodeficiency virus type 1 env genes. The cleavage site between the signal peptide and the external env glycoprotein resides between the cysteine residue at position 21 and the threonine residue at position 22, starting from the first residue after the env gene initiator methionine. The env precursor polyprotein gp160 is cleaved between arginine 526 and glycine 527 to give rise to the external glycoprotein and the transmembrane of SIVMAC.

Citing Articles

Systematic analysis of intracellular trafficking motifs located within the cytoplasmic domain of simian immunodeficiency virus glycoprotein gp41.

Postler T, Bixby J, Desrosiers R, Yuste E PLoS One. 2014; 9(12):e114753.

PMID: 25479017 PMC: 4257708. DOI: 10.1371/journal.pone.0114753.

Understanding the process of envelope glycoprotein incorporation into virions in simian and feline immunodeficiency viruses.

Affranchino J, Gonzalez S Viruses. 2014; 6(1):264-83.

PMID: 24441862 PMC: 3917442. DOI: 10.3390/v6010264.

The tale of the long tail: the cytoplasmic domain of HIV-1 gp41.

Postler T, Desrosiers R J Virol. 2012; 87(1):2-15.

PMID: 23077317 PMC: 3536369. DOI: 10.1128/JVI.02053-12.

Mutations within the putative membrane-spanning domain of the simian immunodeficiency virus transmembrane glycoprotein define the minimal requirements for fusion, incorporation, and infectivity.

West J, Johnston P, Dubay S, Hunter E J Virol. 2001; 75(20):9601-12.

PMID: 11559792 PMC: 114531. DOI: 10.1128/JVI.75.20.9601-9612.2001.

Simian immunodeficiency virus mutants resistant to serum neutralization arise during persistent infection of rhesus monkeys.

Burns D, Collignon C, Desrosiers R J Virol. 1993; 67(7):4104-13.

PMID: 8510218 PMC: 237779. DOI: 10.1128/JVI.67.7.4104-4113.1993.

References

Kanki P, Barin F, Mboup S, Allan J, Marlink R, McLane M . New human T-lymphotropic retrovirus related to simian T-lymphotropic virus type III (STLV-IIIAGM). Science. 1986; 232(4747):238-43. DOI: 10.1126/science.3006256. View

Henderson L, Benveniste R, Sowder R, Copeland T, Schultz A, Oroszlan S . Molecular characterization of gag proteins from simian immunodeficiency virus (SIVMne). J Virol. 1988; 62(8):2587-95. PMC: 253688. DOI: 10.1128/JVI.62.8.2587-2595.1988. View

Fultz P, McClure H, Anderson D, Swenson R, Anand R, Srinivasan A . Isolation of a T-lymphotropic retrovirus from naturally infected sooty mangabey monkeys (Cercocebus atys). Proc Natl Acad Sci U S A. 1986; 83(14):5286-90. PMC: 323936. DOI: 10.1073/pnas.83.14.5286. View

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

Allan J, Coligan J, Barin F, McLane M, Sodroski J, Rosen C . Major glycoprotein antigens that induce antibodies in AIDS patients are encoded by HTLV-III. Science. 1985; 228(4703):1091-4. DOI: 10.1126/science.2986290. View

Kanki P, McLane M, King Jr N, Letvin N, Hunt R, Sehgal P . Serologic identification and characterization of a macaque T-lymphotropic retrovirus closely related to HTLV-III. Science. 1985; 228(4704):1199-201. DOI: 10.1126/science.3873705. View

Daniel M, Letvin N, King N, Kannagi M, Sehgal P, Hunt R . Isolation of T-cell tropic HTLV-III-like retrovirus from macaques. Science. 1985; 228(4704):1201-4. DOI: 10.1126/science.3159089. View

Veronese F, Devico A, Copeland T, Oroszlan S, Gallo R, Sarngadharan M . Characterization of gp41 as the transmembrane protein coded by the HTLV-III/LAV envelope gene. Science. 1985; 229(4720):1402-5. DOI: 10.1126/science.2994223. View

Letvin N, Daniel M, Sehgal P, Desrosiers R, Hunt R, Waldron L . Induction of AIDS-like disease in macaque monkeys with T-cell tropic retrovirus STLV-III. Science. 1985; 230(4721):71-3. DOI: 10.1126/science.2412295. View

10.

Benveniste R, Arthur L, Tsai C, Sowder R, Copeland T, Henderson L . Isolation of a lentivirus from a macaque with lymphoma: comparison with HTLV-III/LAV and other lentiviruses. J Virol. 1986; 60(2):483-90. PMC: 288916. DOI: 10.1128/JVI.60.2.483-490.1986. View

11.

Hirsch V, Riedel N, Mullins J . The genome organization of STLV-3 is similar to that of the AIDS virus except for a truncated transmembrane protein. Cell. 1987; 49(3):307-19. DOI: 10.1016/0092-8674(87)90283-2. View

12.

Franchini G, Gurgo C, Guo H, Gallo R, Collalti E, Fargnoli K . Sequence of simian immunodeficiency virus and its relationship to the human immunodeficiency viruses. Nature. 1987; 328(6130):539-43. DOI: 10.1038/328539a0. View

13.

Chakrabarti L, Guyader M, Alizon M, Daniel M, Desrosiers R, Tiollais P . Sequence of simian immunodeficiency virus from macaque and its relationship to other human and simian retroviruses. Nature. 1987; 328(6130):543-7. DOI: 10.1038/328543a0. View

14.

Ohta Y, Masuda T, Tsujimoto H, Ishikawa K, Kodama T, Morikawa S . Isolation of simian immunodeficiency virus from African green monkeys and seroepidemiologic survey of the virus in various non-human primates. Int J Cancer. 1988; 41(1):115-22. DOI: 10.1002/ijc.2910410121. View

15.

Murphey-Corb M, Martin L, RANGAN S, Baskin G, Gormus B, Wolf R . Isolation of an HTLV-III-related retrovirus from macaques with simian AIDS and its possible origin in asymptomatic mangabeys. Nature. 1986; 321(6068):435-7. DOI: 10.1038/321435a0. View