Contribution of Eukaryotic-type Serine/threonine Kinase to Stress Response and Virulence of Streptococcus Suis

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 Mar 19

PMID 24637959

Citations 19

Authors

Haodan Zhu

Junming Zhou

Yanxiu Ni

Zhengyu Yu

Aihua Mao

Yiyi Hu

Wei Wang

Xuehan Zhang

Libin Wen

Bin Li

Xiaomin Wang

Yang Yu

Lixin Lv

Rongli Guo

Chengping Lu

Kongwang He

Affiliations

Soon will be listed here.

Abstract

Streptococcus suis serotype 2 (SS2) is an important swine and human pathogen responsible for septicemia and meningitis. The bacterial homologues of eukaryotic-type serine/threonine kinases (ESTKs) have been reported to play critical roles in various cellular processes. To investigate the role of STK in SS2, an isogenic stk mutant strain (Δstk) and a complemented strain (CΔstk) were constructed. The Δstk showed a significant decrease in adherence to HEp-2 cells, compared with the wild-type strain, and a reduced survival ratio in whole blood. In addition, the Δstk exhibited a notable reduced tolerance of environmental stresses including high temperature, acidic pH, oxidative stress, and high osmolarity. More importantly, the Δstk was attenuated in both the CD1 mouse and piglet models of infection. The results of quantitative reverse transcription-PCR (qRT-PCR) analysis indicated that the expressions of a few genes involving in adherence, stress response and virulence were clearly decreased in the Δstk mutant strain. Our data suggest that SsSTK is required for virulence and stress response in SS2.

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