Anion Channels in Giant Liposomes Made of Endoplasmic Reticulum Vesicles from Rat Exocrine Pancreas

Overview

Journal J Membr Biol

Date 1988 Sep 1

PMID 2463365

Citations 21

Authors

A Schmid

H Gogelein

T P Kemmer

I Schulz

Affiliations

Soon will be listed here.

Abstract

Using the method of dehydration and rehydration, rough endoplasmic reticulum (RER) vesicles, isolated by differential centrifugation, can be enlarged to giant liposomes with diameters ranging from 5 to 200 micron. Patch-clamp studies on these giant RER liposomes revealed the existence of a channel with a mean conductance of 260 +/- 7 pS (n = 23; 140 mmol/liter KCl on both sides). The channel is about four times more permeable for Cl- than for K+. Its activity is strongly voltage regulated. At low potentials (+/- 20 mV) the channel is predominantly in its open state with an open probability near 1.0, whereas it closes permanently at high positive and negative voltages (+/- 70 mV). The channel activity is not influenced by changing the free Ca2+ concentration from 1 mmol/liter to less than 10(-9) mol/liter on either side, and is also not affected by typical Cl- -channel blockers like diphenylamine-2-carboxylate (DPC, 1 mmol/liter) or 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid (SITS, 1 mmol/liter). Another chloride channel with a single-channel conductance of 79 +/- 6 pS (n = 4) was less frequently observed. In the potential range of -80 to +40 mV this channel displayed no voltage-dependent gating. We assume that these anion channels are involved in the maintenance of electroneutrality during Ca2+ uptake in the RER.

Citing Articles

Loss of Clcc1 results in ER stress, misfolded protein accumulation, and neurodegeneration.

Jia Y, Jucius T, Cook S, Ackerman S J Neurosci. 2015; 35(7):3001-9.

PMID: 25698737 PMC: 4331624. DOI: 10.1523/JNEUROSCI.3678-14.2015.

The maxi-anion channel: a classical channel playing novel roles through an unidentified molecular entity.

Sabirov R, Okada Y J Physiol Sci. 2009; 59(1):3-21.

PMID: 19340557 PMC: 10717152. DOI: 10.1007/s12576-008-0008-4.

ATP release via anion channels.

Sabirov R, Okada Y Purinergic Signal. 2008; 1(4):311-28.

PMID: 18404516 PMC: 2096548. DOI: 10.1007/s11302-005-1557-0.

GOLAC: an endogenous anion channel of the Golgi complex.

Nordeen M, Jones S, Howell K, Caldwell J Biophys J. 2000; 78(6):2918-28.

PMID: 10827972 PMC: 1300877. DOI: 10.1016/S0006-3495(00)76832-9.

Single-channel properties of a rat brain endoplasmic reticulum anion channel.

Clark A, Murray D, Ashley R Biophys J. 1997; 73(1):168-78.

PMID: 9199781 PMC: 1180918. DOI: 10.1016/S0006-3495(97)78057-3.

References

Hamill O, Marty A, Neher E, Sakmann B, Sigworth F . Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981; 391(2):85-100. DOI: 10.1007/BF00656997. View

Mueller P, Chien T, Rudy B . Formation and properties of cell-size lipid bilayer vesicles. Biophys J. 1983; 44(3):375-81. PMC: 1434845. DOI: 10.1016/S0006-3495(83)84311-2. View

Fox J . Ion channel subconductance states. J Membr Biol. 1987; 97(1):1-8. DOI: 10.1007/BF01869609. View

Wangemann P, Wittner M, Di Stefano A, Englert H, Lang H, Schlatter E . Cl(-)-channel blockers in the thick ascending limb of the loop of Henle. Structure activity relationship. Pflugers Arch. 1986; 407 Suppl 2:S128-41. DOI: 10.1007/BF00584942. View

Montal M . Reconstitution of channel proteins from excitable cells in planar lipid bilayer membranes. J Membr Biol. 1987; 98(2):101-15. DOI: 10.1007/BF01872123. View

Hanrahan J, Alles W, Lewis S . Single anion-selective channels in basolateral membrane of a mammalian tight epithelium. Proc Natl Acad Sci U S A. 1985; 82(22):7791-5. PMC: 391420. DOI: 10.1073/pnas.82.22.7791. View

Blatz A, Magleby K . Single voltage-dependent chloride-selective channels of large conductance in cultured rat muscle. Biophys J. 1983; 43(2):237-41. PMC: 1329253. DOI: 10.1016/S0006-3495(83)84344-6. View

Tank D, Miller C, Webb W . Isolated-patch recording from liposomes containing functionally reconstituted chloride channels from Torpedo electroplax. Proc Natl Acad Sci U S A. 1982; 79(24):7749-53. PMC: 347425. DOI: 10.1073/pnas.79.24.7749. View

Krouse M, Schneider G, Gage P . A large anion-selective channel has seven conductance levels. Nature. 1986; 319(6048):58-60. DOI: 10.1038/319058a0. View

10.

Nelson D, Tang J, Palmer L . Single-channel recordings of apical membrane chloride conductance in A6 epithelial cells. J Membr Biol. 1984; 80(1):81-9. DOI: 10.1007/BF01868692. View

11.

Imamura K, Schulz I . Phosphorylated intermediate of (Ca2+ + K+)-stimulated Mg2+-dependent transport ATPase in endoplasmic reticulum from rat pancreatic acinar cells. J Biol Chem. 1985; 260(20):11339-47. View

12.

Streb H, Irvine R, Berridge M, Schulz I . Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate. Nature. 1983; 306(5938):67-9. DOI: 10.1038/306067a0. View

13.

Streb H, Bayerdorffer E, Haase W, Irvine R, Schulz I . Effect of inositol-1,4,5-trisphosphate on isolated subcellular fractions of rat pancreas. J Membr Biol. 1984; 81(3):241-53. DOI: 10.1007/BF01868717. View

14.

Woll K, Leibowitz M, Neumcke B, Hille B . A high-conductance anion channel in adult amphibian skeletal muscle. Pflugers Arch. 1987; 410(6):632-40. DOI: 10.1007/BF00581324. View

15.

Gogelein H, Koepsell H . Channels in planar bilayers made from commercially available lipids. Pflugers Arch. 1984; 401(4):433-4. DOI: 10.1007/BF00584351. View

16.

Criado M, Keller B . A membrane fusion strategy for single-channel recordings of membranes usually non-accessible to patch-clamp pipette electrodes. FEBS Lett. 1987; 224(1):172-6. DOI: 10.1016/0014-5793(87)80442-8. View

17.

Lindau M, Fernandez J . A patch-clamp study of histamine-secreting cells. J Gen Physiol. 1986; 88(3):349-68. PMC: 2228826. DOI: 10.1085/jgp.88.3.349. View

18.

Ochs D, Korenbrot J, Williams J . Intracellular free calcium concentrations in isolated pancreatic acini; effects of secretagogues. Biochem Biophys Res Commun. 1983; 117(1):122-8. DOI: 10.1016/0006-291x(83)91549-8. View

19.

Maruyama Y, Peterson O . Single-channel currents in isolated patches of plasma membrane from basal surface of pancreatic acini. Nature. 1982; 299(5879):159-61. DOI: 10.1038/299159a0. View

20.

Colombini M . Purification of VDAC (voltage-dependent anion-selective channel) from rat liver mitochondria. J Membr Biol. 1983; 74(2):115-21. DOI: 10.1007/BF01870500. View