Single Anion-selective Channels in Basolateral Membrane of a Mammalian Tight Epithelium

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Nov 1

PMID 2415972

Citations 39

Authors

J W Hanrahan

W P Alles

S A Lewis

Affiliations

Soon will be listed here.

Abstract

Basolateral membrane chloride permeability of surface cells from rabbit urinary bladder epithelium was studied using the patch-clamp technique. Two types of anion-selective channel were observed. One channel type showed inward rectification and had a conductance of 64 pS at-50 mV when bathed symmetrically by saline solution containing 150 mM chloride; the other resembled high-conductance voltage-dependent anion channels (VDACs). Both channels had the selectivity sequence Cl-approximately equal to Br-approximately equal to I- approximately equal to SCN- approximately equal to NO3- greater than F- greater than acetate greater than gluconate greater than Na+ approximately equal to K+ and were sensitive to the anion exchange inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. Basolateral chloride conductance in urinary bladder is apparently due to the 64 pS anion channel, which is active at physiological potentials. Imperfect selectivity of this channel against cations might also account for the low, but finite, sodium permeability of the basolateral membrane.

Citing Articles

The properties, functions, and pathophysiology of maxi-anion channels.

Sabirov R, Merzlyak P, Islam M, Okada T, Okada Y Pflugers Arch. 2016; 468(3):405-20.

PMID: 26733413 DOI: 10.1007/s00424-015-1774-5.

The maxi-anion channel: a classical channel playing novel roles through an unidentified molecular entity.

Sabirov R, Okada Y J Physiol Sci. 2009; 59(1):3-21.

PMID: 19340557 PMC: 10717152. DOI: 10.1007/s12576-008-0008-4.

ATP release via anion channels.

Sabirov R, Okada Y Purinergic Signal. 2008; 1(4):311-28.

PMID: 18404516 PMC: 2096548. DOI: 10.1007/s11302-005-1557-0.

Okadaic acid-sensitive activation of Maxi Cl(-) channels by triphenylethylene antioestrogens in C1300 mouse neuroblastoma cells.

Diaz M, Bahamonde M, Lock H, Munoz F, Hardy S, Posas F J Physiol. 2001; 536(Pt 1):79-88.

PMID: 11579158 PMC: 2278843. DOI: 10.1111/j.1469-7793.2001.00079.x.

Electrodiffusion, barrier, and gating analysis of DIDS-insensitive chloride conductance in human red blood cells treated with valinomycin or gramicidin.

Freedman J, Novak T J Gen Physiol. 1997; 109(2):201-16.

PMID: 9041449 PMC: 2220065. DOI: 10.1085/jgp.109.2.201.

References

Greger R, Schlatter E . Properties of the basolateral membrane of the cortical thick ascending limb of Henle's loop of rabbit kidney. A model for secondary active chloride transport. Pflugers Arch. 1983; 396(4):325-34. DOI: 10.1007/BF01063938. View

Hamill O, Marty A, Neher E, Sakmann B, Sigworth F . Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981; 391(2):85-100. DOI: 10.1007/BF00656997. View

Petersen K, Reuss L . Cyclic AMP-induced chloride permeability in the apical membrane of Necturus gallbladder epithelium. J Gen Physiol. 1983; 81(5):705-29. PMC: 2216558. DOI: 10.1085/jgp.81.5.705. View

Grinstein S, Clarke C, Dupre A, Rothstein A . Volume-induced increase of anion permeability in human lymphocytes. J Gen Physiol. 1982; 80(6):801-23. PMC: 2228648. DOI: 10.1085/jgp.80.6.801. View

Schein S, Colombini M, Finkelstein A . Reconstitution in planar lipid bilayers of a voltage-dependent anion-selective channel obtained from paramecium mitochondria. J Membr Biol. 1976; 30(2):99-120. DOI: 10.1007/BF01869662. View

Larsen E, Kristensen P . Properties of a conductive cellular chloride pathway in the skin of the toad (Bufo bufo). Acta Physiol Scand. 1978; 102(1):1-21. DOI: 10.1111/j.1748-1716.1978.tb06041.x. View

Blatz A, Magleby K . Single voltage-dependent chloride-selective channels of large conductance in cultured rat muscle. Biophys J. 1983; 43(2):237-41. PMC: 1329253. DOI: 10.1016/S0006-3495(83)84344-6. View

Larsen E, Rasmussen B . Chloride channels in toad skin. Philos Trans R Soc Lond B Biol Sci. 1982; 299(1097):413-34. DOI: 10.1098/rstb.1982.0141. View

Nelson D, Tang J, Palmer L . Single-channel recordings of apical membrane chloride conductance in A6 epithelial cells. J Membr Biol. 1984; 80(1):81-9. DOI: 10.1007/BF01868692. View

10.

Lindemann B, Van Driessche W . Sodium-specific membrane channels of frog skin are pores: current fluctuations reveal high turnover. Science. 1977; 195(4275):292-4. DOI: 10.1126/science.299785. View

11.

Lewis S, Wills N, Eaton D . Basolateral membrane potential of a tight epithelium: ionic diffusion and electrogenic pumps. J Membr Biol. 1978; 41(2):117-48. DOI: 10.1007/BF01972629. View

12.

Klyce S, Wong R . Site and mode of adrenaline action on chloride transport across the rabbit corneal epithelium. J Physiol. 1977; 266(3):777-99. PMC: 1283591. DOI: 10.1113/jphysiol.1977.sp011793. View

13.

Nagel W . The intracellular electrical potential profile of the frog skin epithelium. Pflugers Arch. 1976; 365(2-3):135-43. DOI: 10.1007/BF01067010. View

14.

Coronado R, Latorre R . Detection of K+ and Cl-channels from calf cardiac sarcolemma in planar lipid bilayer membranes. Nature. 1982; 298(5877):849-52. DOI: 10.1038/298849a0. View

15.

Ferreira K, FERREIRA H . The regulation of volume and ion composition in frog skin. Biochim Biophys Acta. 1981; 646(2):193-202. DOI: 10.1016/0005-2736(81)90325-4. View

16.

Lewis S, Ifshin M, Loo D, Diamond J . Studies of sodium channels in rabbit urinary bladder by noise analysis. J Membr Biol. 1984; 80(2):135-51. DOI: 10.1007/BF01868770. View

17.

GOLDMAN D . POTENTIAL, IMPEDANCE, AND RECTIFICATION IN MEMBRANES. J Gen Physiol. 2009; 27(1):37-60. PMC: 2142582. DOI: 10.1085/jgp.27.1.37. View

18.

Colombini M . A candidate for the permeability pathway of the outer mitochondrial membrane. Nature. 1979; 279(5714):643-5. DOI: 10.1038/279643a0. View

19.

GRAY P, Bevan S, Ritchie J . High conductance anion-selective channels in rat cultured Schwann cells. Proc R Soc Lond B Biol Sci. 1984; 221(1225):395-409. DOI: 10.1098/rspb.1984.0041. View

20.

Kolb H, Brown C, Murer H . Identification of a voltage-dependent anion channel in the apical membrane of a Cl(-)-secretory epithelium (MDCK). Pflugers Arch. 1985; 403(3):262-5. DOI: 10.1007/BF00583597. View