Recognition of Oligonucleotide-encoded T Cell Epitopes Introduced into a Gene Unrelated to the Original Antigen

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1989 Jan 1

PMID 2462610

Citations 16

Authors

G Chimini

P Pala

J Sire

B R Jordan

J L Maryanski

Affiliations

Soon will be listed here.

Abstract

We have previously demonstrated that H-2Kd-restricted CTL specific for HLA-CW3 or HLA-A24 can recognize synthetic peptides corresponding to residues 170-182 of the HLA molecules. Synthetic oligonucleotides encoding region 170-182 of CW3 or A24 were inserted into the influenza nucleoprotein (NP) gene. We demonstrate herein that P815 (H-2d) cells transfected with the NP-oligo recombinant genes are specifically lysed by HLA-specific Kd-restricted CTL clones. Our results imply that there must be a high degree of flexibility for the expression of T cell epitopes in different molecular contexts.

Citing Articles

Presentation of a T cell receptor antagonist peptide by immunoglobulins ablates activation of T cells by a synthetic peptide or proteins requiring endocytic processing.

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A multivalent minigene vaccine, containing B-cell, cytotoxic T-lymphocyte, and Th epitopes from several microbes, induces appropriate responses in vivo and confers protection against more than one pathogen.

An L, Whitton J J Virol. 1997; 71(3):2292-302.

PMID: 9032365 PMC: 191338. DOI: 10.1128/JVI.71.3.2292-2302.1997.

Epitope-specific tolerance induction with an engineered immunoglobulin.

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Efficient loading of identical viral peptide onto class II molecules by antigenized immunoglobulin and influenza virus.

Brumeanu T, Swiggard W, Steinman R, Bona C, Zaghouani H J Exp Med. 1993; 178(5):1795-9.

PMID: 8228825 PMC: 2191225. DOI: 10.1084/jem.178.5.1795.

Simian virus 40 T antigen as a carrier for the expression of cytotoxic T-lymphocyte recognition epitopes.

Fu T, Bonneau R, Tevethia M, Tevethia S J Virol. 1993; 67(11):6866-71.

PMID: 7692088 PMC: 238134. DOI: 10.1128/JVI.67.11.6866-6871.1993.

References

Corradin G, Chiller J . Lymphocyte specificity to protein antigens. II. Fine specificity of T-cell activation with cytochrome c and derived peptides as antigenic probes. J Exp Med. 1979; 149(2):436-47. PMC: 2184807. DOI: 10.1084/jem.149.2.436. View

Zinkernagel R, Doherty P . MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness. Adv Immunol. 1979; 27:51-177. DOI: 10.1016/s0065-2776(08)60262-x. View

Auffray C, Rougeon F . Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980; 107(2):303-14. DOI: 10.1111/j.1432-1033.1980.tb06030.x. View

Zinkernagel R, Rosenthal K . Experiments and speculation on antiviral specificity of T and B cells. Immunol Rev. 1981; 58:131-55. DOI: 10.1111/j.1600-065x.1981.tb00352.x. View

Shimonkevitz R, Kappler J, Marrack P, Grey H . Antigen recognition by H-2-restricted T cells. I. Cell-free antigen processing. J Exp Med. 1983; 158(2):303-16. PMC: 2187345. DOI: 10.1084/jem.158.2.303. View

Townsend A, McMichael A, Carter N, HUDDLESTON J, Brownlee G . Cytotoxic T cell recognition of the influenza nucleoprotein and hemagglutinin expressed in transfected mouse L cells. Cell. 1984; 39(1):13-25. DOI: 10.1016/0092-8674(84)90187-9. View

Townsend A, Gotch F, Davey J . Cytotoxic T cells recognize fragments of the influenza nucleoprotein. Cell. 1985; 42(2):457-67. DOI: 10.1016/0092-8674(85)90103-5. View

Van Pel A, De Plaen E, Boon T . Selection of highly transfectable variant from mouse mastocytoma P815. Somat Cell Mol Genet. 1985; 11(5):467-75. DOI: 10.1007/BF01534840. View

Babbitt B, Allen P, Matsueda G, Haber E, UNANUE E . Binding of immunogenic peptides to Ia histocompatibility molecules. Nature. 1985; 317(6035):359-61. DOI: 10.1038/317359a0. View

10.

Maryanski J, Moretta A, Jordan B, De Plaen E, Van Pel A, Boon T . Human T cell recognition of cloned HLA class I gene products expressed on DNA transfectants of mouse mastocytoma P815. Eur J Immunol. 1985; 15(11):1111-7. DOI: 10.1002/eji.1830151109. View

11.

Morrison L, Lukacher A, Braciale V, Fan D, Braciale T . Differences in antigen presentation to MHC class I-and class II-restricted influenza virus-specific cytolytic T lymphocyte clones. J Exp Med. 1986; 163(4):903-21. PMC: 2188068. DOI: 10.1084/jem.163.4.903. View

12.

Townsend A, Rothbard J, Gotch F, Bahadur G, Wraith D, McMichael A . The epitopes of influenza nucleoprotein recognized by cytotoxic T lymphocytes can be defined with short synthetic peptides. Cell. 1986; 44(6):959-68. DOI: 10.1016/0092-8674(86)90019-x. View

13.

Maryanski J, Accolla R, Jordan B . H2-restricted recognition of cloned HLA class I gene products expressed in mouse cells. J Immunol. 1986; 136(12):4340-7. View

14.

Buus S, Colon S, Smith C, Freed J, Miles C, Grey H . Interaction between a "processed" ovalbumin peptide and Ia molecules. Proc Natl Acad Sci U S A. 1986; 83(11):3968-71. PMC: 323646. DOI: 10.1073/pnas.83.11.3968. View

15.

Townsend A, Bastin J, Gould K, Brownlee G . Cytotoxic T lymphocytes recognize influenza haemagglutinin that lacks a signal sequence. Nature. 1986; 324(6097):575-7. DOI: 10.1038/324575a0. View

16.

Maryanski J, Pala P, Corradin G, Jordan B, Cerottini J . H-2-restricted cytolytic T cells specific for HLA can recognize a synthetic HLA peptide. Nature. 1986; 324(6097):578-9. DOI: 10.1038/324578a0. View

17.

Maryanski J, Pala P, Cerottini J, Corradin G . Synthetic peptides as antigens and competitors in recognition by H-2-restricted cytolytic T cells specific for HLA. J Exp Med. 1988; 167(4):1391-405. PMC: 2188917. DOI: 10.1084/jem.167.4.1391. View

18.

Pala P, Corradin G, Strachan T, Sodoyer R, Jordan B, Cerottini J . Mapping of HLA epitopes recognized by H-2-restricted cytotoxic T lymphocytes specific for HLA using recombinant genes and synthetic peptides. J Immunol. 1988; 140(3):871-7. View