A Proteomic Chronology of Gene Expression Through the Cell Cycle in Human Myeloid Leukemia Cells

Overview

Journal Elife

Specialty Biology

Date 2014 Mar 6

PMID 24596151

Citations 74

Authors

Tony Ly

Yasmeen Ahmad

Adam Shlien

Dominique Soroka

Allie Mills

Michael J Emanuele

Michael R Stratton

Angus I Lamond

Affiliations

Soon will be listed here.

Abstract

Technological advances have enabled the analysis of cellular protein and RNA levels with unprecedented depth and sensitivity, allowing for an unbiased re-evaluation of gene regulation during fundamental biological processes. Here, we have chronicled the dynamics of protein and mRNA expression levels across a minimally perturbed cell cycle in human myeloid leukemia cells using centrifugal elutriation combined with mass spectrometry-based proteomics and RNA-Seq, avoiding artificial synchronization procedures. We identify myeloid-specific gene expression and variations in protein abundance, isoform expression and phosphorylation at different cell cycle stages. We dissect the relationship between protein and mRNA levels for both bulk gene expression and for over ∼6000 genes individually across the cell cycle, revealing complex, gene-specific patterns. This data set, one of the deepest surveys to date of gene expression in human cells, is presented in an online, searchable database, the Encyclopedia of Proteome Dynamics (http://www.peptracker.com/epd/). DOI: http://dx.doi.org/10.7554/eLife.01630.001.

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