Basic Fibroblast Growth Factor from Human Keratinocytes is a Natural Mitogen for Melanocytes

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1988 Oct 1

PMID 2459134

Citations 74

Authors

R Halaban

R Langdon

N Birchall

C Cuono

A Baird

G Scott

G Moellmann

J McGuire

Affiliations

Soon will be listed here.

Abstract

To survive and proliferate in pure culture, human melanocytes require basic fibroblast growth factor (bFGF) and cAMP. Without these factors, even in the presence of serum, the cells die. Melanocytes cultured in the presence of keratinocytes, however, survive for weeks without added bFGF and cAMP. We show here that the growth factor for melanocytes produced by human keratinocytes is bFGF because its activity can be abolished by neutralizing antibodies to bFGF and by a bFGF synthetic peptide that inhibits the binding of the growth factor to its receptor. The melanocyte mitogen in keratinocytes is cell associated and increases after irradiation with ultraviolet B. Northern blots reveal bFGF gene transcripts in keratinocytes but not melanocytes. These studies demonstrate that bFGF elaborated by keratinocytes in vitro sustains melanocyte growth and survival, and they suggest that keratinocyte-derived bFGF is the natural growth factor for normal human melanocytes in vivo.

Citing Articles

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