A Disposable Alkaline Phosphatase-based Biosensor for Vanadium Chronoamperometric Determination

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2014 Feb 27

PMID 24569772

Citations 1

Authors

Ana Lorena Alvarado-Gamez

Maria Asuncion Alonso-Lomillo

Olga Dominguez-Renedo

Maria Julia Arcos-Martinez

Affiliations

Soon will be listed here.

Abstract

A chronoamperometric method for vanadium ion determination, based on the inhibition of the enzyme alkaline phosphatase, is reported. Screen-printed carbon electrodes modified with gold nanoparticles were used as transducers for the immobilization of the enzyme. The enzymatic activity over 4-nitrophenyl phosphate sodium salt is affected by vanadium ions, which results in a decrease in the chronoamperometric current registered. The developed method has a detection limit of 0.39 ± 0.06 µM, a repeatability of 7.7% (n = 4) and a reproducibility of 8% (n = 3). A study of the possible interferences shows that the presence of Mo(VI), Cr(III), Ca(II) and W(VI), may affect vanadium determination at concentration higher than 1.0 mM. The method was successfully applied to the determination of vanadium in spiked tap water.

Citing Articles

A chronoamperometric screen printed carbon biosensor based on alkaline phosphatase inhibition for W(IV) determination in water, using 2-phospho-L-ascorbic acid trisodium salt as a substrate.

Alvarado-Gamez A, Alonso-Lomillo M, Dominguez-Renedo O, Arcos-Martinez M Sensors (Basel). 2015; 15(2):2232-43.

PMID: 25621602 PMC: 4367303. DOI: 10.3390/s150202232.

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